Carotenoid analogs or derivatives for controlling connexin 43 expression

ABSTRACT

A method of controlling (e.g., influencing or affecting) connexin 43 expression in a subject may include administering to the subject an effective amount of a pharmaceutically acceptable formulation. In some embodiments, controlling connexin 43 expression in a subject may effectively treat cardiac arrhythmia and/or cancerous and pre-cancerous cells in a subject. The pharmaceutically acceptable formulation may include a synthetic analog or derivative of a carotenoid. The subject may be administered a carotenoid analog or derivative, either alone or in combination with another carotenoid analog or derivative, or co-antioxidant formulation. The carotenoid analog may include a conjugated polyene with between 7 to 14 double bonds. The conjugated polyene may include an acyclic alkene including at least one substituent and/or a cyclic ring including at least one substituent. In some embodiments, a carotenoid analog or derivative may include at least one substituent.

PRIORITY CLAIM

This application is a continuation in part of patent application Ser.No. 10/629,538 entitled “Structural Carotenoid Analogs for theInhibition and Amelioration of Disease” filed on Jul. 29, 2003 whichclaims priority to Provisional Patent Application No. 60/399,194entitled “Structural Carotenoid Analogs for the Inhibition andAmelioration of Reperfusion Injury” filed on Jul. 29, 2002; ProvisionalPatent Application No. 60/467,973 entitled “Structural CarotenoidAnalogs for the Inhibition and Amelioration of Disease” filed on May 5,2003; Provisional Patent Application No. 60/472,831 entitled “StructuralCarotenoid Analogs for the Inhibition and Amelioration of Disease” filedon May 22, 2003; Provisional Patent Application No. 60/473,741 entitled“Structural Carotenoid Analogs for the Inhibition and Amelioration ofDisease” filed on May 28, 2003; and Provisional Patent Application No.60/485,304 entitled “Structural Carotenoid Analogs for the Inhibitionand Amelioration of Disease” filed on Jul. 3, 2003.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The invention generally relates to the fields of medicinal and syntheticchemistry. More specifically, the invention relates to the synthesis anduse of carotenoid analogs or derivatives.

2. Description of the Relevant Art

Cardiovascular disease (CVD), and specifically coronary artery disease(CAD), remains the leading cause of death in the United States andworldwide. CVD is a leading cause of mortality and morbidity in theworld. Small to moderate reductions in cardiovascular risk, which leadto decreased emergency department visits and hospitalizations for acutecoronary syndromes, can yield substantial clinical and public healthbenefits.

Extensive research with antioxidants has shown that they are effectivetherapeutic agents in the primary and secondary prevention ofcardiovascular disease. CVD remains the leading cause of death for allraces in the U.S.; now, approximately 60 million Americans have someform of CVD. Life expectancy in the U.S. would increase by almost 7years if CVD could be eliminated. The absolute number of deaths due toCVD has fallen since 1996; however, it remains the single largest causeof death in the United States, with a total annual healthcare burden ofgreater than $300 billion (including heart attack and stroke).

Ischemia is the lack of an adequate oxygenated blood supply to aparticular tissue. Ischemia underlies many acute and chronic diseasestates including, but not limited to:

-   -   Myocardial infarction, or MI    -   Unstable angina    -   Stable angina pectoris    -   Abrupt reclosure following percutaneous transluminal coronary        angioplasty (PTCA)    -   Thrombotic stroke (85% of the total number of strokes)    -   Embolic vascular occlusion    -   Peripheral vascular insufficiency    -   Organ transplantation    -   Deep venous thrombosis, or DVT    -   Indwelling catheter occlusion        Ischemia may also become a problem in elective procedures such        as: scheduled organ transplantation; scheduled coronary artery        bypass graft surgery (CABG); and scheduled percutaneous        transluminal coronary angioplasty (PTCA). Common to each of        these settings is the phenomenon of reperfusion injury: the        production of reactive oxygen species (ROS) upon reintroduction        of oxygenated blood flow to a previously ischemic area, with        subsequent paradoxical additional tissue damage. In particular,        the use(s) of thrombolytic therapy in acute myocardial        infarction (AMI) and acute thrombotic stroke—as well as surgical        revascularization with PTCA—are typically associated with the        reperfusion of ischemic myocardium and/or brain. Clinical        outcome is improved with the achievement of early patency after        acute thrombosis, however, not without cost (i.e., “reperfusion        injury”).

Current therapy allows for reperfusion with pharmacologic agents,including recombinant tissue-type plasminogen activator (r-TPA),Anistreplase (APSAC), streptokinase, and urokinase. Recent studies haveshown the best clinical outcome after AMI occurs with early surgicalreperfusion. However, surgical reperfusion is available at only 15 to 20percent of care centers in the United States, and much fewer worldwide.It is likely, therefore, that pharmacologic reperfusion will remainclinically relevant and important for the foreseeable future.Thrombolytic therapy is unsuccessful in reperfusion of about 20% ofinfarcted arteries. Of the arteries that are successfully reperfused,approximately 15% abruptly reclose (within 24 hours). Measures ofsystemic inflammation (e.g., serum levels of C-reactive protein or CRP)correlate strongly with clinical reclosure in these patients. Myocardialsalvage appears to be maximal in a 2 to 6 hour “therapeutic window”subsequent to acute plaque rupture and thrombosis. In acute thromboticor thromboembolic stroke, this therapeutic window is even narrower,generally less than 3 hours post-thrombosis. Recombinant tissue-typeplasminogen activator administered within 3 hours of ischemic strokesignificantly improves clinical outcome, but increases the risk ofhemorrhage.

During a period of ischemia, many cells undergo the biochemical andpathological changes associated with anoxia but remain potentiallyviable. These potentially viable cells are therefore the “battleground”in the reperfusion period. Ischemia creates changes in the affectedtissue, with the potential final result of contraction band and/orcoagulation necrosis of at-risk myocardium. Pathologic changes inischemic myocardium include, but are not limited to:

-   -   Free radical and ROS production    -   ATP loss and defective ATP resynthesis    -   Creatine phosphate loss    -   Extracellular potassium loss    -   Active tension-generating capacity loss of myocardium    -   Cellular swelling    -   Acidosis    -   Loss of ionic homeostasis    -   Structural disorganization    -   Electrical instability and arrhythmogenesis    -   Lipid membrane peroxidation    -   Glutathione and other endogenous/exogenous antioxidant depletion        (including vitamins C and E and carotenoids)        Rescue of ischemic myocardium that has not irreversibly reached        the threshold of necrosis is the focus of intervention in        ischemia-reperfusion injury.

Gap junctions are a unique type of intercellular junction found in mostanimal cell types. They form aqueous channels that interconnect thecytoplasms of adjacent cells and enable the direct intercellularexchange of small (less than approximately 1 kiloDalton) cytoplasmiccomponents. Gap junctions are created across the interveningextracellular space by the docking of two hemichannels (“connexons”)contributed by each adjacent cell. Each hemichannel of is an oligomer ofsix connexin molecules.

Connexin 43 was the second connexin gene discovered and it encodes oneof the most widely expressed connexins in established cell lines andtissues. Gap junctions formed by connexin 43 have been implicated indevelopment, cardiac function, and growth control.

One common manifestation of CVD is cardiac arrhythmia. Cardiacarrhythmia is generally considered a disturbance of the electricalactivity of the heart that manifests as an abnormality in heart rate orheart rhythm. Patients with a cardiac arrhythmia may experience a widevariety of symptoms ranging from palpitations, to fainting (“syncope”),and sudden cardiac death.

The major connexin in the cardiovascular system is connexin 43. Gapjunctional coordination of cellular responses among cells of thevascular wall, in particular the endothelial cells, is thought to becritical for the local modulation of vasomotor tone and for themaintenance of circulatory homeostasis. Controlling the upregulation ofconnexin 43 may also assist in the maintenance of electrical stabilityin cardiac tissue. Maintaining electrical stability in cardiac tissuemay benefit the health of hundreds of thousands of people a year withsome types of cardiovascular disease [e.g., ischemic heart disease (IHD)and arrhythmia], and may prevent the occurrence of sudden cardiac deathin patients at high risk for arrhythmia.

Cancer is generally considered to be characterized by the uncontrolled,abnormal growth of cells. Connexin 43, as previously mentioned, is alsoassociated with cellular growth control. Growth control by connexin 43is likely due to connexin 43's association with gap junctionalcommunication. Maintenance, restoration, or increases of functional gapjunctional communication inhibits the proliferation of transformedcells. Therefore, upregulation and/or control of the availability ofconnexin 43 may potentially inhibit and/or ameliorate the spread ofcancerous cells.

Chronic liver injury, regardless of etiology, may lead to a progressivespectrum of pathology from acute and chronic inflammation, to earlystage fibrosis, and finally to cirrhosis, end-stage liver disease(ESRD), and hepatocellular carcinoma (HCC). A cascade of inflammatoryevents secondary to the initiating injury, including the release ofcytokines and the formation of reactive oxygen species (ROS), activateshepatic stellate cells (HSC). HSC produce extracellular matrixcomponents (ECM), including collagen, and are critical in the processwhich generates hepatic fibrosis.

End-stage liver disease [manifested as either cirrhosis orhepatocellular carcinoma (HCC)] is the eighth leading cause ofdisease-related death in the United States. Chronic inflammation in theliver resulting from viral infection, alcohol abuse, drug-inducedtoxicity, iron and copper overload, and many other factors can initiatehepatic fibrosis. By-products of hepatocellular damage activate Kupffercells, which then release a number of cytokines, ROS (including inparticular superoxide anion), and other paracrine and autocrine factorswhich in turn act upon hepatic stellate cells (HSC). It is now believedthat the lynchpin cell in the fibrogenetic cascade is the HSC, the celltype responsible for the production of ECM. In vitro evidencedemonstrates that ROS can induce HSC cells. Elevated levels of indirectmarkers of oxidative stress (e.g., thiobarbituric acid reactive speciesor TBARS) are observed in all patients with chronic liver disease. Inaddition, levels of gluthathione, glutathione peroxidase, superoxidedismutase, carotenoids, and α-tocopherol (vitamin E) are significantlylower in patients with chronic liver disease. Supplying these endogenousand/or exogenous antioxidants reverses many of the signs of chronicliver disease, including both surrogate markers for the disease process,as well as direct measurements of hepatic fibrosis. Therefore, they arelikely potent agents for therapeutic intervention in liver disease.

SUMMARY

In some embodiments, the administration of structural analogs orderivatives of carotenoids may inhibit and/or ameliorate the occurrenceof diseases in subjects. Maladies which may be treated with structuralanalogs or derivatives of carotenoids may include any disease thatinvolves production of reactive oxygen species and/or other radical andnon-radical species (for example singlet oxygen, a reactive oxygenspecies but not a radical). In some embodiments, water-soluble analogsof carotenoids may be used to treat a disease that involves productionof reactive oxygen species. Oxidation of DNA, proteins, and lipids byreactive oxygen species and other radical and non-radical species hasbeen implicated in a host of human diseases. Radicals may be the primarycause for the following conditions, may make the body more susceptibleto other disease-initiating factors, may inhibit endogenous defenses andrepair processes, and/or may enhance the progression of incipientdisease(s). The administration of structural analogs or derivatives ofcarotenoids by one skilled in the art—including consideration of thepharmacokinetics and pharmacodynamics of therapeutic drug delivery—isexpected to inhibit and/or ameliorate said disease conditions. In thefirst category are those disease conditions in which a single organ isprimarily affected, and for which evidence exists that radicals and/ornon-radicals are involved in the pathology of the disease. Theseexamples are not to be seen as limiting, and additional diseaseconditions will be obvious to those skilled in the art.

-   -   Head, Eyes, Ears, Nose, and Throat: age-related macular        degeneration (ARMD), retinal detachment, hypertensive retinal        disease, uveitis, choroiditis, vitreitis, ocular hemorrhage,        degenerative retinal damage, cataractogenesis and cataracts,        retinopathy of prematurity, Meuniere's disease, drug-induced        ototoxicity (including aminoglycoside and furosemide toxicity),        infectious and idiopathic otitis, otitis media, infectious and        allergic sinusitis, head and neck cancer;    -   Central Nervous System (brain and spinal cord): senile dementia        (including Alzheimer's dementia), Neuman-Pick's disease,        neurotoxin reactions, hyperbaric oxygen effects, Parkinson's        disease, cerebral and spinal cord trauma, hypertensive        cerebrovascular injury, stroke (thromboembolic, thrombotic, and        hemorrhagic), infectious encephalitis and meningitis, allergic        encephalomyelitis and other demyelinating diseases, amyotrophic        lateral sclerosis (ALS), multiple sclerosis, neuronal ceroid        lipofuscinoses, ataxia-telangiectasia syndrome, aluminum, iron,        and other heavy metal(s) overload, primary brain        carcinoma/malignancy and brain metastases;    -   Cardiovascular: arteriosclerosis, atherosclerosis, peripheral        vascular disease, myocardial infarction, chronic stable angina,        unstable angina, idiopathic surgical injury (during CABG, PTCA),        inflammatory heart disease [as measured and influenced by        C-reactive protein (CRP) and myeloperoxidase (MPO)], vascular        restenosis, low-density lipoprotein oxidation (ox-LDL),        cardiomyopathies, cardiac arrhythmia (ischemic and        post-myocardial infarction induced), congestive heart failure        (CHF), drug toxicity (including adriamycin and doxorubicin),        Keshan disease (selenium deficiency), trypanosomiasis, alcohol        cardiomyopathy, venous stasis and injury (including deep venous        thrombosis or DVT), thrombophlebitis;    -   Pulmonary: asthma, reactive airways disease, chronic obstructive        pulmonary disease (COPD or emphysema), hyperoxia, hyperbaric        oxygen effects, cigarette smoke inhalation effects,        environmental oxidant pollutant effects, acute respiratory        distress syndrome (ARDS), bronchopulmonary dysplasia, mineral        dust pneumoconiosis, adriamycin toxicity, bleomycin toxicity,        paraquat and other pesticide toxicities, chemical pneumonitis,        idiopathic pulmonary interstitial fibrosis, infectious pneumonia        (including fungal), sarcoidosis, asbestosis, lung cancer (small-        and large-cell), anthrax infection, anthrax toxin exposure;    -   Renal: hypertensive renal disease, end-stage renal disease,        diabetic renal disease, infectious glomerulonephritis, nephrotic        syndrome, allergic glomerulonephritis, type I-IV        hypersensitivity reactions, renal allograft rejection, nephritic        antiglomerular basement membrane disease, heavy metal        nephrotoxicity, drug-induced (including aminoglycoside,        furosemide, and non-steroidal anti-inflammatory) nephrotoxicity,        rhabdomyolisis, renal carcinoma;    -   Hepatic: carbon tetrachloride liver injury, endotoxin and        lipopolysaccharide liver injury, chronic viral infection        (including Hepatitis infection), infectious hepatitis (non-viral        etiology), hemachromatosis, Wilson's disease, acetaminophen        overdose, congestive heart failure with hepatic congestion,        cirrhosis (including alcoholic, viral, and idiopathic        etiologies), hepatocellular carcinoma, hepatic metastases;    -   Gastrointestinal: inflammatory bowel disease (including Crohn's        disease, ulcerative colitis, and irritable bowel syndrome),        colon carcinoma, polyposis, infectious diverticulitis, toxic        megacolon, gastritis (including Helicobacter pylori infection),        gastric carcinoma, esophagitis (including Barrett's esophagus),        gastro-esophageal reflux disease (GERD), Whipple's disease,        gallstone disease, pancreatitis, abetalipoproteinemia,        infectious gastroenteritis, dysentery, nonsteroidal        anti-inflammatory drug-induced toxicity;    -   Hematopoietic/Hematologic: Pb (lead) poisoning, drug-induced        bone marrow suppression, protoporphyrin photo-oxidation,        lymphoma, leukemia, porphyria(s), parasitic infection (including        malaria), sickle cell anemia, thallasemia, favism, pernicious        anemia, Fanconi's anemia, post-infectious anemia, idiopathic        thrombocytopenic purpura (ITP), autoimmune deficiency syndrome        (AIDS);    -   Genitourinary: infectious prostatitis, prostate carcinoma,        benign prostatic hypertrophy (BPH), urethritis, orchitis,        testicular torsion, cervicitis, cervical carcinoma, ovarian        carcinoma, uterine carcinoma, vaginitis, vaginismus;    -   Musculoskeletal: osteoarthritis, rheumatoid arthritis,        tendonitis, muscular dystrophy, degenerative disc disease,        degenerative joint disease, exercise-induced skeletal muscle        injury, carpal tunnel syndrome, Guillan-Barre syndrome, Paget's        disease of bone, ankylosing spondilitis, heterotopic bone        formation; and    -   Integumentary: solar radiation injury (including sunburn),        thermal injury, chemical and contact dermatitis (including Rhus        dermatitis), psoriasis, Bloom syndrome, leukoplakia        (particularly oral), infectious dermatitis, Kaposi's sarcoma.

In the second category are multiple-organ conditions whose pathology hasbeen linked convincingly in some way to radical and non-radical injury:aging, including age-related immune deficiency and premature agingdisorders, cancer, cardiovascular disease, cerebrovascular disease,radiation injury, alcohol-mediated damage (includingWernicke-Korsakoff's syndrome), ischemia-reperfusion damage,inflammatory and auto-immune disease, drug toxicity, amyloid disease,overload syndromes (iron, copper, etc.), multi-system organ failure, andendotoxemia/sepsis.

Maladies, which may be treated with structural carotenoid analogs orderivatives, may include, but are not limited to, cardiovascularinflammation, hepatitis C infection, cancer (hepatocellular carcinomaand prostate), macular degeneration, rheumatoid arthritis, stroke,Alzheimer's disease, and/or osteoarthritis. In an embodiment, theadministration of water soluble analogs or derivatives of carotenoids toa subject may inhibit and/or ameliorate the occurrence ofischemia-reperfusion injury in subjects. In some embodiments, watersoluble and other structural carotenoid analogs or derivatives may beadministered to a subject alone or in combination with other structuralcarotenoid analogs or derivatives. The occurrence ofischemia-reperfusion injury in a human subject that is experiencing, orhas experienced, or is predisposed to experience myocardial infarction,stroke, peripheral vascular disease, venous or arterial occlusion and/orrestenosis, organ transplantation, coronary artery bypass graft surgery,percutaneous transluminal coronary angioplasty, and cardiovasculararrest and/or death may be inhibited or ameliorated by theadministration of therapeutic amounts of water soluble and/or otherstructural carotenoid analogs or derivatives to the subject.

“Water soluble” structural carotenoid analogs or derivatives are thoseanalogs or derivatives which may be formulated in aqueous solution,either alone or with excipients. Water soluble carotenoid analogs orderivatives may include those compounds and synthetic derivatives whichform molecular self-assemblies, and may be more properly termed “waterdispersible” carotenoid analogs or derivatives. Water soluble and/or“water-dispersible” carotenoid analogs or derivatives may be preferredin some embodiments of the current invention.

Water soluble carotenoid analogs or derivatives may have a watersolubility of greater than about 1 mg/mL in some embodiments. In certainembodiments, water soluble carotenoid analogs or derivatives may have awater solubility of greater than about 10 mg/mL. In some embodiments,water soluble carotenoid analogs or derivatives may have a watersolubility of greater than about 50 mg/mL.

In an embodiment, the administration of water soluble analogs orderivatives of carotenoids to a subject may inhibit and/or amelioratesome types of cardiovascular disease associated with cardiac arrhythmia.In some embodiments, water soluble analogs or derivatives of carotenoidsmay be administered to a subject alone or in combination with othercarotenoid analogs or derivatives. Carotenoid analogs or derivatives mayassist in the maintenance of electrical stability in cardiac tissue.Assistance in the maintenance of electrical stability in cardiac tissuemay inhibit and/or ameliorate some types of cardiovascular disease,including in particular sudden cardiac death attributable to lethalcardiac arrhythmia.

In an embodiment, the administration of water soluble analogs orderivatives of carotenoids to a subject may inhibit and/or amelioratethe occurrence of liver disease in the subject. In some embodiments,water soluble analogs or derivatives of carotenoids may be administeredto a subject alone or in combination with other carotenoid analogs orderivatives. The liver disease may be a chronic liver disease such as,for example, Hepatitis C infection.

In an embodiment, the administration of water soluble analogs orderivatives of carotenoids to a subject may inhibit and/or amelioratethe proliferation and propagation of initiated, transformed and/orcancerous or pre-cancerous cell(s). In some embodiments, water solubleanalogs or derivatives of carotenoids may be administered to a subjectalone or in combination with other carotenoid analogs or derivatives.Carotenoid analogs or derivatives may inhibit the proliferation rate ofcarcinogen-initiated cells. Carotenoid analogs or derivatives mayincrease connexin 43 expression. Increase of connexin 43 expression mayincrease, maintain, or restore gap junctional intercellularcommunication and thus inhibit the growth of carcinogen-initiated cells.

Embodiments may be further directed to pharmaceutical compositionscomprising combinations of structural carotenoid analogs or derivativesto said subjects. The composition of an injectable structural carotenoidanalog or derivative of astaxanthin may be particularly useful in thetherapeutic methods described herein. In yet a further embodiment, aninjectable astaxanthin structural analog or derivative is administeredwith another astaxanthin structural analog or derivative and/or othercarotenoid structural analogs or derivatives, or in formulation withother antioxidants and/or excipients that further the intended purpose.In some embodiments, one or more of the astaxanthin structural analogsor derivatives are water soluble.

As used herein, terms such as carotenoid analog and carotenoidderivative may generally refer to in some embodiments chemical compoundsor compositions derived from a naturally occurring carotenoid. In someembodiments, terms such as carotenoid analog and carotenoid derivativemay generally refer to chemical compounds or compositions which aresynthetically derived from non-carotenoid based parent compounds;however, which ultimately substantially resemble a carotenoid derivedanalog. In certain embodiments, terms such as carotenoid analog andcarotenoid derivative may generally refer to a synthetic derivative of anaturally occurring carotenoid.

In an embodiment, a chemical compound including a carotenoid derivativemay have the general structure (I):

Each R³ may be independently hydrogen or methyl. R¹ and R² may beindependently H, an acyclic alkene with one or more substituents, or acyclic ring including one or more substituents. y may be 5 to 12. Insome embodiments, y may be about 3 to about 15. In certain embodiments,the maximum value of y may only be limited by the ultimate size of thechemical compound, particularly as it relates to the size of thechemical compound and the potential interference with the chemicalcompound's biological availability as discussed herein. In someembodiments, substituents may be at least partially hydrophilic. In someembodiment, substituents may be each independently coupled to acarotenoid analog or derivative via an ether and/or an esterfunctionality. These carotenoid derivatives may be used in apharmaceutical composition.

In an embodiment, a chemical compound including a carotenoid derivativemay have the general structure (Ia):

Each R may be independently hydrogen or methyl. R¹ and R² may beindependently H, an acyclic alkene with one or more substituents, or acyclic ring including one or more substituents. In some embodiments,substituents may be at least partially hydrophilic. These carotenoidderivatives may be used in a pharmaceutical composition. In oneembodiment, a pharmaceutical composition that includes carotenoidstructural analogs or derivatives having general structure (Ia) may beused for treating ischemia-reperfusion injury.

As used herein, the terms “disodium salt disuccinate astaxanthinderivative”, “dAST”, “Cardax”, “Cardax™”, “rac”, and “astaxanthindisuccinate derivative (ADD)” represent varying nomenclature for the useof the disodium salt disuccinate astaxanthin derivative in variousstereoisomer and aqueous formulations, and represent illustrativeembodiments for the intended use of this structural carotenoid analog.The diacid disuccinate astaxanthin derivative (astaCOOH) is theprotonated form of the derivative utilized for flash photolysis studiesfor direct comparison with non-esterified, “racemic” (i.e., mixture ofstereoisomers) astaxanthin. “Cardax-C” is the disodium salt disuccinatedi-vitamin C derivative (derivative XXIII) utilized in superoxide anionscavenging experiments assayed by electron paramagnetic resonance (EPR)spectroscopy.

BRIEF DESCRIPTION OF THE DRAWINGS

The above brief description as well as further objects, features andadvantages of the methods and apparatus of the present invention will bemore fully appreciated by reference to the following detaileddescription of presently preferred but nonetheless illustrativeembodiments in accordance with the present invention when taken inconjunction with the accompanying drawings.

FIG. 1 depicts a graphic representation of several examples of “parent”carotenoid structures as found in nature.

FIG. 2 depicts an effect of disodium salt disuccinate astaxanthinderivative on the reactive oxygen species superoxide anion as monitoredusing electron paramagnetic resonance (EPR) spectroscopy.

FIG. 3 depicts an effect of a disodium salt disuccinate astaxanthinderivative/free vitamin C solution on the reactive oxygen speciessuperoxide anion as monitored using electron paramagnetic resonance(EPR) spectroscopy.

FIG. 4 depicts a graphical representation of a relative reduction ofinfarct size in male Sprague-Dawley rats with pre-treatment using adisodium salt disuccinate astaxanthin derivative intravenous formulation(Cardax™).

FIG. 5 depicts the chemical structure of the all-trans (all-E) disodiumsalt disuccinate ester derivative of meso-astaxanthin (3R,3′S- or3S,3′R-dihydroxy-β,β-carotene-4,4′-dione; dAST) synthesized for thecurrent study (shown as the all-E dianionic bolamphiphile).

FIG. 6 depicts the ultraviolet-visible absorption spectrum of DAST inethanol at 25° C. (cell length 1 cm, c=1.05×10⁻⁵ M). Molar absorptioncoefficients are shown in parentheses. The second derivative curve ofthe absorption spectrum indicates the exact position of peaks in thenear-UV region and the hidden vibrational fine structure of the mainband.

FIG. 7 depicts the absorption spectrum of dAST in Ringer buffer (pH 7.4,cell length 1 cm, c=1.85×10⁻⁵ M, t=37° C.). Molar absorptioncoefficients are indicated.

FIG. 8 depicts the induced CD and UV/Vis spectra obtained by titrationof human serum albumin (HSA) with DAST in Ringer buffer solution (pH7.4) at low UP ratios. Concentration of HSA was 1.6×10⁻⁴ M and theligand was added as aliquots of DMSO stock solution (cell length 1 cm,t=37° C.). Curves measured at different LRP values are shown. Insets:molar circular dichroic absorption coefficients (Δε in M⁻¹cm⁻¹) andmolar absorption coefficients (6 in M⁻cm⁻¹) of the induced CD andabsorption bands calculated on the basis of total meso-carotenoidconcentration in the solution.

FIG. 9 depicts the induced CD and UV/Vis spectra obtained by titrationof HSA with DAST in Ringer buffer solution (pH 7.4) above L/P ratioof 1. Concentration of HSA was 2.3×10⁻⁴ M and the ligand was added asaliquots of DMSO stock solution (cell length 1 cm, t=37° C.). Curvesmeasured at L/P values of 1.2, 2.0, 2.9, 4.1, 5.7 and 7.4 are shown. CDintensities increase in parallel with the ligand concentration.

FIG. 10 depicts the induced CD and UV/Vis spectra obtained by titrationof HSA with dAST in 0.1 M pH 7.4 phosphate buffer solution above L/Pratio of 1. Concentration of HSA was 2.2×10⁻⁴ M and the ligand was addedas aliquots of DMSO stock solution (cell length 1 cm, t=37° C.). Curvesmeasured at L/P values of 1.2, 2.0, 2.9, 4.1, 5.7, 9.0, 10.6 and 13.1are shown. CD intensities increase in parallel with the ligandconcentration.

FIG. 11A-FIG. 11C depicts an illustration of right-handed chiralarrangements of two meso-carotenoid molecules for which excitonicinteractions produce long-wavelength positive and short-wavelengthnegative Cotton effects in the CD spectrum. Gray-colored molecules liebehind the plane of the paper.

FIG. 12 depicts (upper figure): fluorescence quenching of HSA by dASTmeasured in 0.1 M pH 7.4 phosphate buffer solution at 37 ° C. Initialand final concentrations of HSA and the ligand were varied between4.2×10⁻⁶ M-4.0×10⁻⁶ M and 1.3×10⁻⁶ M-1.4×10⁻⁵ M, respectively. L/Pratios are noted on curves. The lower figure depicts an effect of DMSOalone on the intrinsic fluorescence of HSA.

FIG. 13 depicts the X-ray crystallographic structure of fatty acid-freeHSA. Subdomains and the two primary drug-binding sites of HSA areindicated. Dotted bar represents spatial dimension of the interdomaincleft, and asterisk indicates the position of Trp214. The inter-atomicdistance between the 3 and 3′ chiral carbon atoms of the dAST moleculeis 28 Å.

FIG. 14A-FIG. 14F depicts that the statistical mixture of stereoisomersof the disodium salt disuccinate astaxanthin derivative (“rac” in FigureLegends) induces functional gap junctional communication in murineembryonic fibroblast (10T1/2) cells. Confluent cultures were treated for4 days as described in text, then assayed for the ability to transferthe fluorescent dye Lucifer Yellow. Arrows indicate the cell injectedwith Lucifer Yellow.

FIG. 15 A depicts connexin 43 protein expression in cells treated withthe mixture of stereoisomers of the disodium salt disuccinateastaxanthin derivatives as assessed by quantitative Western blotanalysis. The upper bands are believed to represent the phosphorylatedforms of the protein assembled into gap junctions; lower bandsunassembled proteins (Saez, 1998). Lane 1: 1:2 ethanol (EtOH)/H₂O(solvent only negative control); Lane 2: TTNPB, a synthetic retinoid, inacetone at 10⁻⁸ M (positive control); Lane 3: Retinyl acetate in acetoneat 10⁻⁵ M (positive control); Lane 4: Statistical mixture (“rac”) ofstereoisomers of the disodium salt disuccinate astaxanthin derivative at10⁻⁵ M delivered in a 1:2 formulation of EtOH/H₂O; Lane 5:3R,3′R-disodium salt disuccinate astaxanthin derivative at 10-⁵ Mdelivered in a 1:2 formulation of EtOH/H₂O; Lane 6: 3S,3′S disodium saltdisuccinate astaxanthin derivative at 10⁻⁵ M delivered in a 1:2formulation of EtOH/H₂O; and Lane 7: Meso (3R,3′S) disodium saltdisuccinate astaxanthin derivative at 10⁻⁵ M delivered in a 1:2formulation of EtOH/H₂O.

FIG. 15B depicts an immunoblot stained with Coomassie blue todemonstrate equal protein loading of all the bands. This confirms thatdifferences in immunolabeling are not an artifact due to variability intotal protein loaded and/or transferred to the membrane.

FIG. 15C depicts digital analysis of relative induction levels ofconnexin 43 protein expression by the disodium salt disuccinateastaxanthin derivative(s) versus positive and solvent-only treatedcontrols. Lanes as in FIG. 15A. The fold induction is normalized tocontrol levels of Cx43 expression in the 1:2 EtOH/H₂O treated negativecontrols set to an arbitrary unit=1.0.

FIG. 15D depicts the dose-response curve of Cx43 protein expression inmurine embryonic fibroblast cells (10T1/2) treated with the statisticalmixture of stereoisomers of the disodium salt disuccinate astaxanthinderivatives as assessed by quantitative Western blot analysis. The upperbands are believed to represent the phosphorylated forms of the proteinassembled into gap junctions; lower bands unassembled proteins. Lane 1:1:2 EtOH/H₂O (solvent only negative control). Lane 2: TTNPB in acetoneat 10-8 M (positive control). Lane 3: disodium salt disuccinateastaxanthin derivative (“rac”) at 10⁻⁵ M delivered in a 1:2 formulationof EtOH/H₂O. Lane 4: disodium salt disuccinate astaxanthin derivative(“rac”) at 5×10⁻⁶ M delivered in a 1:2 formulation of EtOH/H₂O. Lane 5:disodium salt disuccinate astaxanthin derivative (“rac”) at 10⁻⁶ Mdelivered in a 1:2 formulation of EtOH/H₂O.

FIG. 15E depicts digital analysis of relative induction levels ofconnexin 43 protein expression by the statistical mixture ofstereoisomers of the disodium salt disuccinate astaxanthin derivativeversus positive and solvent-only treated controls. Lanes as in FIG. 15D.The fold induction is normalized to control levels of Cx43 expression inthe 1:2 EtOH/H₂O treated controls set to an arbitrary unit=1.0.

FIG. 16A-FIG. 16F depicts that the statistical mixture of stereoisomersof the disodium salt disuccinate astaxanthin derivative increases theassembly of Cx43 immunoreactive junctional plaques. Confluent culturesof 10T1/2 cells were treated for 4 days as described above with thestatistical mixture of stereoisomers of the disodium salt disuccinateastaxanthin derivative: (1) at 10 ⁻⁵M in 1:2 EtOH/H₂O; (2) with 1:2EtOH/H₂O as solvent only negative control; or (3) TTNPB at 10⁻⁸M intetrahydrofuran (THF) solvent as positive control. Cells wereimmunostained with a Cx43 antibody as described in text. FIG. 16A: thestatistical mixture of stereoisomers of the disodium salt disuccinateastaxanthin derivative at 10⁻⁵M in 1:2 EtOH/H₂O; FIG. 16C: 1:2 EtOH/H₂Oas solvent control; FIG. 16E: TTNPB at 10⁻⁸M in tetrahydrofuran (THF)solvent as positive control. FIGS. 16B, D, and F: digital analysis ofFIGS. 16A, C, and E, respectively, demonstrating pixels above a fixedset threshold positive for fluorescent intensity. Light gray arrows:immunoreactive junctional plaques; dark gray arrows: position of cellnuclei. Note the greater number and intensity of junctionalimmunoreactive plaques in the cultures treated with the statisticalmixture of stereoisomers of the disodium salt disuccinate astaxanthinderivative in comparison with solvent-only treated controls. Thejunctional plaques shown in FIGS. 16C and D represent infrequent plaquesseen in controls; most cells in these cultures were negative for Cx43staining.

FIG. 17 depicts the 3 stereoisomers of the disodium disuccinate diesterof astaxanthin synthesized for the current studies (shown as the all-Egeometric isomers); the mixture of stereoisomers, or individualstereoisomers, were used in separate applications (see Figure legends).Note that the meso forms (3R,3′S and 3S,3′R) are identical.

FIG. 18 depicts the mean percent inhibition of superoxide anion signalas detected by DEPMPO spin trap by the disodium disuccinate derivativesof astaxanthin in pure aqueous formulation. Mixture=statistical mixtureof stereoisomers [3S,3′S, meso (3R,3′S and 3′R,3S), 3R,3′R in a 1:2:1ratio]. Each derivative in aqueous formulation was standardized tocontrol EPR signal detected without addition of compound (set at 0%inhibition by convention). Note the absence of superoxide inhibition by3S,3′S formulation in water. In each case, the aqueous formulation isless potent than the corresponding formulation in EtOH (MG. 19).

FIG. 19 depicts the mean percent inhibition of superoxide anion signalas detected by DEPMPO spin trap by the disodium disuccinate derivativesof astaxanthin in ethanolic formulation. Mixture=statistical mixture ofstereoisomers [3S,3′S, meso (3R,3′S and 3′R,3S), 3R,3′R in a 1:2:1ratio]. The mixture, meso, and 3R,3′R stock solutions were 1:2ethanol/water (33⅓% EtOH); the 3S,3′S stock solution was 1:1ethanol/water (50% EtOH). Final concentration of EtOH in the isolatedneutrophil test assay was 0.3% and 0.5%, respectively. Each derivativein ethanolic formulation was standardized to control EPR signal detectedwithout addition of compound (set at 0% inhibition by convention).

FIG. 20 depicts the mean percent inhibition of superoxide anion signalas detected by DEPMPO spin trap by the mixture of stereoisomers of thedisodium disuccinate derivative of astaxanthin (tested in 1:2 EtOH/waterformulation; final EtOH concentration in isolated neutrophil assay0.3%). As the concentration of the derivative increases, inhibitionincreases in a non-linear, dose-dependent manner. At 3 mM, near-completeinhibition of superoxide anion signal is seen (95.0% inhibition).

FIG. 21 depicts the mean percent inhibition of superoxide anion signalas detected by DEPMPO spin trap by the hydrochloride salt dilysineastaxanthin derivative. This derivative was highly water soluble (>50mg/mL), and did not require a co-solvent for excellent radical-quenchingability in this assay. Compare the superoxide anion inhibition of thisderivative with that depicted in FIG. 20, for a derivative that formssupramolecular assemblies in pure aqueous formulation.

FIG. 22 depicts a standard plot of concentration of non-esterified, freeastaxanthin versus time for plasma after single dose oral gavage inblack mice. Only non-esterified, free astaxanthin is detected in plasma,corroborating the complete de-esterification of the carotenoid analog orderivative in the mammalian gut.

FIG. 23 depicts a standard plot of concentration of non-esterified, freeastaxanthin verses time for liver after single dose oral gavage in blackmice. Only non-esterified, free astaxanthin is detected in liver, alsocorroborating (see FIG. 22 for plasma) the complete de-esterification ofthe carotenoid analog or derivative in the mammalian gut, as has beendescribed previously. At every time point, liver levels ofnon-esterified, free astaxanthin are greater than that observed inplasma, a finding suggesting vastly improved solid-organ delivery offree carotenoid in the novel emulsion vehicle used in this study.

FIG. 24 depicts the effect of the disodium disuccinate astaxanthinderivative at 500 mg/kg by oral gavage on lipopolysaccharide(LPS)-induced liver injury in mice (as measured by elevation in serumalanine aminotransferase, or ALT). Three (3) animals were tested in eachgroup. Control animals received saline alone (sham-treated controls;left portion of figure) or emulsion without disodium disuccinateastaxanthin derivative (vehicle controls). Sham-treated animalsreceiving the novel derivative demonstrated no effect on backgroundlevels of ALT; mice receiving the oral emulsion with the novelderivative at 500 mg/kg showed reduced induced levels of ALT, indicatingprotection against hepatic necrosis after LPS insult.

FIG. 25 depicts a graphical representation of a relative reduction ofinfarct size in male Sprague-Dawley rats with pre-treatment using adisodium salt disuccinate astaxanthin derivative intravenous formulation(Cardax™). A linear relationship between dose and infarct size reductionwas seen. The levels of infarct size reduction approach that observedwith ischemic pre-conditioning.

FIG. 26 depicts a graphical representation of a relative reduction ofinfarct size in male Sprague-Dawley rats with pre-treatment using adisodium salt disuccinate astaxanthin derivative intravenous formulation(Cardax™).

FIG. 27 depicts transient absorption versus delay for the diaciddisuccinate astaxanthin derivative (astaCOOH) using flash photolysis.The experiment was performed in acetonitrile (MeCN) using nitronaftalin(NN) as photosensitizer. The spectra obtained demonstrate that thediacid disuccinate astaxanthin derivative behaves identically tonon-esterified, free racemic astaxanthin as a radical quencher(formation of the carotenoid radical cation), identifying the derivativeas an active “soft-drug” which generates non-esterified, freeastaxanthin in vivo after both oral and intravenous delivery.

FIG. 28 depicts transient absorption versus delay for the referencecompound non-esterified, free racemic astaxanthin (asta)] using flashphotolysis. The experiment was performed in acetonitrile (MeCN) usingnitronaftalin (NN) as photosensitizer. The spectra obtained are nearlysuperimposable on those obtained for the diacid disuccinate astaxanthinderivative (astaCOOH), suggesting identical radical-cation formingproperties for both compounds.

FIG. 29 depicts a pictorial representation of a Western blot of apolyacrylamide gel with anti-connexin 43 antibody.

FIG. 30 depicts a pictorial representation of quantitative densitometricimages of Western blots with anti-connexin 43 antibodies followed by HRPchemiluminescence on a Biorad imager.

FIG. 31 depicts a graph of relative fold-induction of connexin 43expression by positive control (TTNPB, potent synthetic retinoid) andtest compounds (disodium salt disuccinate astaxanthin derivative in fourwater and/or ethanol (EtOH)/water formulations: H₂O-10⁻⁵, H₂O-10⁻⁶,H₂O-10⁻⁷, and EtOH/H₂O-10⁻⁵)versus sterile water control (H₂O) at 96hours post-dosing.

FIG. 32 depicts a graph of mean levels of non-esterified, freeastaxanthin in plasma and liver after eleven (11) days of oral gavage of500 mg/kg disodium disuccinate astaxanthin derivative (ADD) in emulsionvehicle to black mice. Both peak and trough levels in plasma and liverachieved were>200 nM, considered to be protective against oxidativestress and hepatic injury in vivo. The peak levels obtained in liver at6 hours post-11^(th) dose were nearly 9 times the protective levelsnecessary (1760 nM).

FIG. 33 depicts the mean percent inhibition of superoxide anion signalas detected by DEPMPO spin trap by the disodium salt disuccinatedi-vitamin C derivative [derivative (XXIII)]. As the concentration ofthe derivative increases, inhibition increases in a dose-dependentmanner. At 60 μM, nearly complete inhibition of superoxide anion signalis seen. This derivative was also highly water soluble, and wasintroduced into the test assay without a co-solvent (see FIG. 21). Thenovel derivative was comparable in radical-quenching efficacy to theformulation of the disodium salt disuccinate astaxanthin derivative in a1:2 formulation with vitamin C (see FIG. 3), suggesting active,“soft-drug” properties for this derivative. This co-antioxidantderivative strategy increased the relative radical scavenging potency(when compared with the disodium salt disuccinate astaxanthinderivative) by 50-fold.

FIG. 34 depicts effects of non-esterified, free astaxanthin (as theall-trans mixture of stereoisomers) on MCA-induced neoplastictransformation in mouse embryonic fibroblast (10T1/2) cells.Non-esterified, free astaxanthin is produced rapidly in vivo after oraland intravenous administration of novel carotenoid derivatives, and isdetected in high concentration in both plasma and solid organs (see FIG.22 and FIG. 23). Non-esterified, free astaxanthin demonstrated levels ofreduction of neoplastic transformation (100%) above any other carotenoidtested in this assay at similar concentrations, demonstrating theincreased utility of this compound for cancer chemopreventionapplications.

FIG. 35A-FIG. 35C depicts a comparison of an astaxanthin-treated dish tocontrol dishes (see description for FIG. 34).

FIG. 36 depicts a comparison of astaxanthin (as the mixture ofstereoisomers) to previously tested carotenoids in this laboratory usingthis assay (see description for FIG. 34).

FIG. 37 depicts a graphical representation of a relative reduction ofinfarct size in male New Zealand rabbits with pre-treatment using adisodium salt disuccinate astaxanthin derivative intravenous formulation(Cardax™). When compared with the infarct size reduction seen at thesame dose and identical pre-treatment schedule in rodents, a 38%increase in infarct size reduction was observed in the rabbit model.

FIG. 38 depicts a graphical representation of a relative reduction ofcirculating levels of plasma C-reactive protein (CRP) in male NewZealand rabbits with pre-treatment using a disodium disuccinateastaxanthin derivative intravenous formulation (Cardax™). Controlrabbits (saline injection alone) stimulated for the acute-phase responsewith 1% croton oil by subcutaneous injection showed a mean increase of23.5% in circulating CRP levels from baseline (venous sample taken atthe time of reperfusion). In contrast, Cardax™-treated animals (50mg/kg) demonstrated a mean reduction in circulating CRP levels frombaseline (−15.7%), demonstrating the potent anti-inflammatory effects ofCardax™.

While the invention is susceptible to various modifications andalternative forms, specific embodiments thereof are shown by way ofexample in the drawings and may herein be described in detail. Thedrawings may not be to scale. It should be understood, however, that thedrawings and detailed description thereto are not intended to limit theinvention to the particular form disclosed, but on the contrary, theintention is to cover all modifications, equivalents and alternativesfalling within the spirit and scope of the present invention as definedby the appended claims.

DETAILED DESCRIPTION

“Parent” carotenoids may generally refer to those natural compoundsutilized as starting scaffold for structural carotenoid analog orderivative synthesis. Carotenoid derivatives may be derived from anaturally occurring carotenoid. Naturally occurring carotenoids mayinclude lycopene, lycophyll, lycoxanthin, astaxanthin, beta-carotene,lutein, zeaxanthin, and/or canthaxanthin to name a few.

Carotenoids are a group of natural pigments produced principally byplants, yeast, and microalgae. The family of related compounds nownumbers greater than 700 described members, exclusive of Z and Eisomers. Fifty (50) have been found in human sera or tissues. Humans andother animals cannot synthesize carotenoids de novo and must obtain themfrom their diet. All carotenoids share common chemical features, such asa polyisoprenoid structure, a long polyene chain forming thechromophore, and near symmetry around the central double bond.Tail-to-tail linkage of two C₂₀ geranylgeranyl diphosphate moleculesproduces the parent C₄₀ carbon skeleton. Carotenoids without oxygenatedfunctional groups are called “carotenes”, reflecting their hydrocarbonnature; oxygenated carotenes are known as “xanthophylls.” Cyclization atone or both ends of the molecule yields 7 identified end groups(illustrative structures shown in FIG. 1).

Documented carotenoid functions in nature include light-harvesting,photoprotection, and protective and sex-related coloration inmicroscopic organisms, mammals, and birds, respectively. A relativelyrecent observation has been the protective role of carotenoids againstage-related diseases in humans as part of a complex antioxidant networkwithin cells. This role is dictated by the close relationship betweenthe physicochemical properties of individual carotenoids and their invivo functions in organisms. The long system of alternating double andsingle bonds in the central part of the molecule (delocalizing theπ-orbital electrons over the entire length of the polyene chain) confersthe distinctive molecular shape, chemical reactivity, andlight-absorbing properties of carotenoids. Additionally, isomerismaround C═C double bonds yields distinctly different molecular structuresthat may be isolated as separate compounds [known as Z (“cis”) and E(“trans”), or geometric, isomers]. Of the more than 700 describedcarotenoids, an even greater number of the theoretically possible mono-Zand poly-Z isomers are sometimes encountered in nature. The presence ofa Z double bond creates greater steric hindrance between nearby hydrogenatoms and/or methyl groups, so that Z isomers are generally less stablethermodynamically, and more chemically reactive, than the correspondingall-E form. The all-E configuration is an extended, linear, and rigidmolecule. Z-isomers are, by contrast, not simple, linear molecules (theso-called “bent-chain” isomers). The presence of any Z in the polyenechain creates a bent-chain molecule. The tendency of Z-isomers tocrystallize or aggregate is much less than all-E, and Z isomers maysometimes be more readily solubilized, absorbed, and transported in vivothan their all-E counterparts. This has important implications forenteral (e.g., oral) and parenteral (e.g., intravenous, intra-arterial,intramuscular, intraperitoneal, intracoronary, and subcutaneous) dosingin mammals.

Carotenoids with chiral centers may exist either as the R (rectus) or S(sinister) configurations. As an example, astaxanthin (with 2 chiralcenters at the 3 and 3′ carbons) may exist as 3 possible stereoisomers:3S, 3′S; 3R, 3′S and 3S, 3′R (identical meso forms); or 3R, 3′R. Therelative proportions of each of the stereoisomers may vary by naturalsource. For example, Haematococcus pluvialis microalgal meal is 99% 3S,3′S astaxanthin, and is likely the predominant human evolutionary sourceof astaxanthin. Krill (3R,3′R) and yeast sources yield differentstereoisomer compositions than the microalgal source. Syntheticastaxanthin, produced by large manufacturers such as Hoffmann-LaRocheAG, Buckton Scott (USA), or BASF AG, are provided as defined geometricisomer mixtures of a 1:2:1 stereoisomer mixture [3S, 3′S; 3R, 3′S,(meso); 3R, 3′R] of non-esterified, free astaxanthin. Natural sourceastaxanthin from salmonid fish is predominantly a single stereoisomer(3S,3′S), but does contain a mixture of geometric isomers. Astaxanthinfrom the natural source Haematococcus pluvialis may contain nearly 50% Zisomers. As stated above, the Z conformational change may lead to ahigher steric interference between the two parts of the carotenoidmolecule, rendering it less stable, more reactive, and more susceptibleto reactivity at low oxygen tensions. In such a situation, in relationto the all-E form, the Z forms: (1) may be degraded first; (2) maybetter suppress the attack of cells by reactive oxygen species such assuperoxide anion; and (3) may preferentially slow the formation ofradicals. Overall, the Z forms may initially be thermodynamicallyfavored to protect the lipophilic portions of the cell and the cellmembrane from destruction. It is important to note, however, that theall-E form of astaxanthin, unlike β-carotene, retains significant oralbioavailability as well as antioxidant capacity in the form of itsdihydroxy- and diketo-substitutions on the β-ionone rings, and has beendemonstrated to have increased efficacy over β-carotene in most studies.The all-E form of astaxanthin has also been postulated to have the mostmembrane-stabilizing effect on cells in vivo. Therefore, it is likelythat the all-E form of astaxanthin in natural and synthetic mixtures ofstereoisomers is also extremely important in antioxidant mechanisms, andmay be the form most suitable for particular pharmaceuticalpreparations.

The antioxidant mechanism(s) of carotenoids, and in particularastaxanthin, includes singlet oxygen quenching, direct radicalscavenging, and lipid peroxidation chain-breaking. The polyene chain ofthe carotenoid absorbs the excited energy of singlet oxygen, effectivelystabilizing the energy transfer by delocalization along the chain, anddissipates the energy to the local environment as heat. Transfer ofenergy from triplet-state chlorophyll (in plants) or other porphyrinsand proto-porphyrins (in mammals) to carotenoids occurs much morereadily than the alternative energy transfer to oxygen to form thehighly reactive and destructive singlet oxygen (¹O₂). Carotenoids mayalso accept the excitation energy from singlet oxygen if any should beformed in situ, and again dissipate the energy as heat to the localenvironment. This singlet oxygen quenching ability has significantimplications in cardiac ischemia, macular degeneration, porphyria, andother disease states in which production of singlet oxygen has damagingeffects. In the physical quenching mechanism, the carotenoid moleculemay be regenerated (most frequently), or be lost. Carotenoids are alsoexcellent chain-breaking antioxidants, a mechanism important ininhibiting the peroxidation of lipids. Astaxanthin can donate a hydrogen(H.) to the unstable polyunsaturated fatty acid (PUFA) radical, stoppingthe chain reaction. Peroxyl radicals may also, by addition to thepolyene chain of carotenoids, be the proximate cause for lipid peroxidechain termination. The appropriate dose of astaxanthin has been shown tocompletely suppress the peroxyl radical chain reaction in liposomesystems. Astaxanthin shares with vitamin E this dual antioxidant defensesystem of singlet oxygen quenching and direct radical scavenging, and inmost instances (and particularly at low oxygen tension in vivo) issuperior to vitamin E as a radical scavenger and physical quencher ofsinglet oxygen.

Carotenoids, and in particular astaxanthin, are potent direct radicalscavengers and singlet oxygen quenchers and possess all the desirablequalities of such therapeutic agents for inhibition or amelioration ofischemia-reperfusion injury. Synthesis of novel carotenoid derivativeswith “soft-drug” properties (i.e. active as antioxidants in thederivatized form), with physiologically relevant, cleavable linkages topro-moieties, can generate significant levels of free carotenoids inboth plasma and solid organs. In the case of non-esterified, freeastaxanthin, this is a particularly useful embodiment (characteristicsspecific to non-esterified, free astaxanthin below):

-   -   Lipid soluble in natural form; may be modified to become more        water soluble;    -   Molecular weight of 597 Daltons [size<600 daltons (Da) readily        crosses the blood brain barrier, or BBB];    -   Long polyene chain characteristic of carotenoids effective in        singlet oxygen quenching and lipid peroxidation chain breaking;        and    -   No pro-vitamin A activity in mammals (eliminating concerns of        hypervitaminosis A and retinoid toxicity in humans).

The administration of antioxidants which are potent singlet oxygenquenchers and direct radical scavengers, particularly of superoxideanion, should limit hepatic fibrosis and the progression to cirrhosis byaffecting the activation of hepatic stellate cells early in thefibrogenetic pathway. Reduction in the level of ROS by theadministration of a potent antioxidant can therefore be crucial in theprevention of the activation of both HSC and Kupffer cells. Thisprotective antioxidant effect appears to be spread across the range ofpotential therapeutic antioxidants, including water-soluble (e.g.,vitamin C, glutathione, resveratrol) and lipophilic (e.g., vitamin E,β-carotene, astaxanthin) agents. Therefore, a co-antioxidant derivativestrategy in which water-soluble and lipophilic agents are combinedsynthetically is a particularly useful embodiment.

Vitamin E is generally considered the reference antioxidant. Whencompared with vitamin E, carotenoids are more efficient in quenchingsinglet oxygen in homogenenous organic solvents and in liposome systems.They are better chain-breaking antioxidants as well in liposomalsystems. They have demonstrated increased efficacy and potency in vivo.They are particularly effective at low oxygen tension, and in lowconcentration, making them extremely effective agents in diseaseconditions in which ischemia is an important part of the tissue injuryand pathology. These carotenoids also have a natural tropism for theheart and liver after oral administration. Therefore, therapeuticadministration of carotenoids should provide a greater benefit inlimiting fibrosis than vitamin E.

Problems related to the use of some carotenoids and structuralcarotenoid analogs or derivatives include: (1) the complex isomericmixtures, including non-carotenoid contaminants, provided in natural andsynthetic sources leading to costly increases in safety and efficacytests required by such agencies as the FDA; (2) limited bioavailabilityupon administration to a subject; and (3) the differential induction ofcytochrome P450 enzymes (this family of enzymes exhibitsspecies-specific differences which must be taken into account whenextrapolating animal work to human studies). Selection of theappropriate analog or derivative and isomer composition for a particularapplication increases the utility of carotenoid analogs or derivativesfor the uses defined herein.

In an embodiment, the parent carotenoid may have a structure of anynaturally occurring carotenoid. Some examples of naturally occurringcarotenoids that may be used as parent compounds are shown in FIG. 1.

Other non-limiting examples of naturally occuring carotenoids that maybe used as parent compounds may include:

Aaptopurpurin; Actinioerythrin; Actinioerythrol; Adonirubin;Adonixanthin; A.g.470; A.g.471; Agelaxanthin C; Aleuriaxanthin;Alloxanthin; Amarouciaxanthin A; Amarouciaxanthin B; Anchovyxanthin;3′,4′-Anhydrodiatoxanthin; Anhydrodeoxyflexixanthin;Anhydroeschscholtzxanthin; Anhydrolutein; Anhydroperidinin;Anhydrorhodovibrin; Anhydrosaproxanthin; Anhydrowarmingol;Anhydrowarmingone; Antheraxanthin; Aphanicin; Aphanicol; Aphanin;Aphanol; Aphanizophyll; 8′-Apo-β-caroten-8′-al;10′-Apo-β-caroten-10′-al; 12′-Apo-β-caroten-12′-al;14′-Apo-β-caroten-14′-al; 6′-Apo-ψ-caroten-6′-al;8′-Apo-ψ-caroten-8′-al; β-Apo-2-carotenal; β-Apo-3-carotenal;β-Apo-4-carotenal; β-Apo-2′-carotenal; β-Apo-8′-carotenal;β-Apo-10′-carotenal; β-Apo-12′-carotenal; β-Apo-14′-carotenal;Apo-8,8′-carotenedial; 8′-Apo-β-carotene-3,8′-diol;4′-Apo-β-caroten-4′-oic acid; 8′-Apo-β-caroten-8′-oic acid;10′-Apo-β-caroten-10′-oic acid; 12′-Apo-β-caroten-12′-oic acid;β-Apo-2′-carotenoic acid; β-Apo-2′-carotenoic acid methylester;β-Apo-8′-carotenoic acid; β-Apo-10′-carotenoic acid;β-Apo-12′-carotenoic acid; 8′-Apo-β-caroten-3-ol; β-Apo-2′-carotenol;Apo-7-fucoxanthinol; Apo-2-lycopenal; Apo-3-lycopenal; Apo-6′-lycopenal;Apo-8′-lycopenal; Apo-10′-violaxanthal; Apo-12′-violaxanthal;Apoviolaxanthinal; Apo-2-zeaxanthinal; Apo-3-zeaxanthinal;Apo-4-zeaxanthinal; Astacein; Astacene; Astacene dipalmitate;Astaxanthin; Asterinic acid; Asteroidenone; Asym. ζ-carotene;Aurochrome; Auroxanthin; Azafrin; Azafrinaldehyde;

Bacterial phytoene; Bacterioerythrin α; Bacterioerythrin β;Bacteriopurpurin α; Bacterioruberin; α-Bacterioruberin; Bacterioruberindiglycoside; Bacterioruberin monoglycoside; α-Bacterioruberin monomethylether; Bisanhydrobacterioruberin; 3,4,3′,4′-Bisdehydro-β-carotene;Bisdehydrolycopene; 2,2′-Bis(4-hydroxy-3-methyl-2-butenyl)-β, β-cartene;2,2′-Bis[3-(glucosyloxy)-3-methylbutyl]-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene-1,1′-diol;2,2′-Bis[4-(β,D-glucopyranosyloxy)-3-methyl-2-butenyl]-γ,γ-carotene;2,2′-Bis(4-hydroxy-3-methyl-2-butenyl)-γ,γ-carotene;2,2′-Bis(4-hydroxy-3-methyl-2-butenyl)-ε,ε-carotene;2,2′-Bis(3-hydroxy-3-methylbutyl-3,4,3′,4′-tetradehydro-1,2,1′,2′etrahydro-ψ,ψ-carotene-1,1′-diol;2,2′-Bis(3-methyl-2-butenyl)-ε,ε-carotene;2,2′-Bis(3-methyl-2-butenyl-3,4,3′,4′-tetradehydro-1,2-dihydro-ψ,ψ-caroten-1-ol;2,2′-Bis(3-methyl-2-butenyl)-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene-1,1′-diol;2,2′-Bis(3-methyl-2-butenyl)-1,2,1′,2′-tetrahydro-ψ,ψ-carotene-1,1′-diol;2,2′-Bis(O-methyl-5-C-methylpentosyloxy)-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydroψ,ψ-carotene-1,1′-diol;3,3′-Bis(rhamnosyloxy)-β,β-carotene;2,2′-Bis(rhamnosyloxy)-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene-1,1′-diol;Bixin;

Caloxanthin; Calthaxanthin; Canthaxanthin; Capsanthin; Capsanthinepoxide; Capsanthinone; Capsanthone; Capsochrome; Capsorubin;Capsorubindione; Capsorubone; Carangoxanthin;16′-Carboxyl-3′,4′-dehydro-γ-carotene; Carcinoxanthin; Caricaxanthin;β-Carotenal; ψ,ψ-Caroten-20-al; Carotene; Carotene X; α-Carotene;β-Carotene; β,β-Carotene; β,γ-Carotene; β,ε-Carotene; β,φ-Carotene;β,ψ-Carotene; γ-Carotene; γ,γ-Carotene; γ,ψ-Carotene; δ-Carotene;ε-Carotene; ε₁-Carotene; ε,ε-Carotene; ε,ψ-Carotene; ζ-Carotene;ζ-Carotene, asym.; η-Carotene; θ-Carotene; ξ-Carotene; φ-Carotene;φ,φ-Carotene; φ,X-Carotene; φ,ψ-Carotene; X,X-Carotene; ψ-Carotene;ψ,α-Carotene; ψ,ψ-Carotene; θ-Carotene; β-Carotene-5,6,5′,6′-diepoxide;β-Carotene 5,8,5′,8′-di-epoxide; β,β-Carotene-2,2′-diol;β,β-Carotene-2,3-diol; β,β-Carotene-3,4-diol; β,β-Carotene-3,3′-diol;β,β-Carotene-4,4′-diol; β,ε-Carotene-3,2′-diol; β,ε-Carotene-3,3′-diol;β,ψ-Carotene-2,3-diol; β,ψ-Carotene-3,3′-diol; ε,ε-Carotene-3,3′-diol;φ,φ-Carotene-3,3′-diol; ψ,ψ-Carotene-16,16′-diol; β,β-Carotene-3,3′-dioldipalmitate; β,ε-Carotene-3,3′-diol dipalmitate;β,β-Carotene-2,2′-dione; β,β-Carotene-3,4-dione; β,β-Carotene-4,4′dione;β,ψ-Carotene-3,4-dione; ε,ε-Carotene-3,3′-dione;β,χ-Carotene-3′,6′-dione; β,X-Carotene-3,4-dione;β,ψ-Carotene-4,4′-dione; β,φ-Carotene-3,4-dione;ψ,ψ-Carotene-4,4′-dione; α-Carotene 5,6-epoxide; β-Carotene 5,6-epoxide;ζ-Carotene epoxide; Carotene oxide; β,β-Carotene-3,4,3′,4′-tetrol;β,β-Carotene-2,3,2′,3′-tetrol; β,β-Carotene-3,4,3′,4′-tetrone;χ,χ-Carotene-3,6,3′,6′-tetrone; β,β-Carotene-2,3,2′-triol;β,β-Carotene-2,3,3′-triol; β,β-Carotene-3,4,3′-triol;β,β-Carotene-3,4,4′-triol; β,ε-Carotene-3,4,3′-triol;β,ε-Carotene-3,19,3′-triol; β,ε-Carotene-3,20,3′-triol;β,β-Carotene-3,4,4′-trione; β,β-Caroten-2-ol; β,β-Caroten-3-ol;β,β-Caroten-4-ol; β,ε-Caroten-2-ol; β,ε-Caroten-3-ol; β,ε-Caroten-3′-ol;β,ε-Caroten-4-ol; β,φ-Caroten-3-ol; β,X-Caroten-3-ol; β,ψ-Caroten-3-ol;β,ψ-Caroten-4′-ol; ε,ψ-Caroten-3-ol; φ,φ-Caroten-3-ol;ψ,ψ-Caroten-16-ol; ψ,ψ-Caroten-20-ol; Carotenonaldehyd; β-Carotenone;β,β-Caroten-2-one; β,β-Caroten-4-one; β,ε-Caroten-2-one;β,ε-Caroten-4-one; β,ψ-Caroten-4-one; γ-Caroten-4-one; α-Carotone;Celaxanthin; Chiriquixanthin A; Chiriquixanthin B; Chlorellaxanthin;Chlorobactene; Chloroxanthin; Chrysanthemaxanthin; Citranaxanthin;α-Citraurin; β-Citraurin; β-Citraurinene; β-Citraurinol; Citroxanthin;Compound X; C.p.: Corynebacterium poinsettiae; Corynexanthin;Corynexanthin glucoside; C.p.; C.p.; C.p.; Crocetin; γ-Crocetin;Crocetindial(dehyde); Crocetin diglucosyl ester; Crocetin dimethylester; Crocetin gentiobiosyl glucosyl diester; Crocetin glucosyl methyldiester; Crocetin monogentiobiosyl ester; Crocetinsemialdehyde; Crocin;Crocoxanthin; Crustaxanthin; Cryptocapsin; Cryptocapsone; Cryptochrome;α-Cryptoeutreptiellanone; β-Cryptoeutreptiellanone; Cryptoflavin;Cryptomonaxanthin; Cryptoxanthene; Cryptoxanthin; α-Cryptoxanthin;β-Cryptoxanthin; Cryptoxanthin 5,6,5′,6′diepoxide; Cryptoxanthin5,6,5′,8′diepoxide; Cryptoxanthin 5,8,5′,8′diepoxide; Cryptoxanthin5,6-epoxide; Cryptoxanthin 5,8-epoxide; Cryptoxanthol; Cucurbitaxanthin;Cyclic ζ-carotene; Cynthiaxanthin;

Decahydro-β-carotene;1,2,7,8,11,12,7′,8′,11′,12′-Decahydro-ψ,ψ-carotene;7,8,11,12,15,7′,8′,11′,12′,15′Decahydro-ψ,ψ-carotene;1,2,7,8,11,12,7′,8′,11′,12′-Decahydro-ψ, ψ-caroten-1-ol;Decahydrolycopene; Decaprenoxanthin; Decaprenoxanthin diglucoside;Decaprenoxanthin monoglucoside; Deepoxyneoxanthin; Dehydro- see alsoBisdehydro-, Didehydro-, MonodehydroDehydroadonirubin;Dehydroadonixanthin; Dehydrocarotene II; Dehydrocarotene III;Dehydro-β-arotene; 3,4-Dehydro-β-carotene; 3′,4′-Dehydro-γ-carotene;3′,4′-Dehydrocryptoxanthin; Dehydrogenans-P; Dehydrogenans-P;Dehydrogenans-P; Dehydrogenans-P; Dehydrogenans-P 439 mono-OH;dehydrogenans-Phytoene; dehydrogenans-Phytofluene;Dehydrohydroxyechinenone; 3′-Dehydrolutein; 3,4-Dehydrolycopen-16-al;Dehydrolycopene; 3,4-Dehydrolycopene; 15,15′-Dehydrolycopersene;7′,8′,11′,12′-Dehydrononapreno xanthin; 11′,12′-Dehydrononaprenoxanthin;3′,4′-Dehydro-17′(or 18′)-oxo-γ-carotene; Dehydropapilioerythrin;11,12-Dehydrophytoene; 11′,12′-Dehydrophytoene;2′-Dehydroplectaniaxanthin; Dehydroretrocarotene; 3,4-Dehydrorhodopin;Dehydrorhodovibrin; 3′,4′-Dehydrorubixanthin; Dehydrosqualene;7,8,7′,8′-Dehydrozeaxanthin; 7,8-Dehydrozeinoxanthin; Demethyl(ated)spheroidene; Deoxyflexixanthin; Deoxylutein I;Deshydroxydecaprenoxanthin; Diadinochrome; Diadinoxanthin;Dianhydroeschscholtzxanthin; 4,4′-Diapo-ζ-carotene;4,4′-Diapocaroten-4-al; 4,4′-Diapocarotene-4,4′-dial;8,8′-Diapocarotene-8,8′-dial; 6,6′-Diapocarotene-6,6′-dioic acid;8,8′-Diapocarotene-8,8′-dioic acid; 4,4′-Diapocaroten-4-oic acid;4,4′-Diaponeurosporene; 4,4′-Diaponeurosporen-4-oic acid;4,4′-Diapophytoene; 4,4′-Diapophytofluene;4,4′-Diapo-7,8,11,12-tetrahydro lycopene; Diatoxanthin; Didehydro-, seealso Dehydro-, Monodehydro 3′,4′-Didehydro-2′-apo-β-caroten-2′-al;3′,4′-Didehydro-2′-apo-β-caroten-2′-ol; 7,8-Didehydroastaxanthin;3′,4′-Didehydro-β,ψ-caroten-16′-al; 3,4-Didehydro-ψ,ψ-caroten-16-al;3,4-Didehydro-β,β-carotene; 4,4′-Didehydro-β-carotene;3,4-Didehydro-β,ε-carotene; 3,4-Didehydro-β,φ-carotene;3,4-Didehydro-β,X-carotene; 3′,4′-Didehydro-β,ψ-carotene;3′,4′-Didehydro-γ,ψ-carotene; 7,8-Didehydro-φ,φ-carotene;7,8-Didehydro-φ,X-carotene; 3,4-Didehydro-ψ,ψ-carotene;7,8-Didehydro-β,β-carotene-3,3′-diol;7,8-Didehydro-β,ε-carotene-3,3′-diol;3,4-Didehydro-β,β-carotene-2,2′-dione;3′,4′-Didehydro-β,ψ-caroten-16′-oic acid;3′,4′-Didehydro-β,β-caroten-3-ol; 3′,4′-Didehydro-β,62 -caroten-4-ol;7,8-Didehydro-β,ε-caroten-3-ol; 7,8-Didehydro-β,φ-caroten-3-ol;7,8-Didehydro-β,X-caroten-3-ol; 3′,4′-Didehydro-β,ψ-caroten-3-ol;3′,4′-Didehydro-β,ψ-caroten-16′-ol; 3′,4′-Didehydro-β,ψ-caroten-18′-ol;3′,4′-Didehydro-β,β-caroten-4-one; 3′,4′-Didehydro-β,ψ-caroten-4-one;7′,8′-Didehydro-β,β-carotene 3,4,3′-triol;3,4-Didehydro-1,2-dihydro-ψ,ψ-carotene;3,4-Didehydro-1,2-dihydro-ψ,ψ-caroten-20-al;6,7-Didehydro-5,6-dihydro-β,β-carotene-3,3′-diol;3′,4′-Didehydro-1′,2′-dihydro-β,ψ-carotene-3,1′-diol;3′,4′-Didehydro-1′,2′-dihydro-β,ψ-carotene-1,2′-diol;3′,4′-Didehydro-1′,2′-dihydro-β,ψ-carotene-4,2′-dione;3,4-Didehydro-1,2-dihydro-ψ,ψ-carotene-1,2-diol;7′,8′-Didehydro-5,6-dihydro-β,β-carotene-3,5,6,3′-tetrol;6,7-Didehydro-5,6-dihydro-β,β-carotene-3,5,3′-triol;7′,8′-Didehydro-5,6-dihydro-β,β-carotene-3,5,3′-triol;3′,4′-Didehydro-1′,2′-dihydro-β,ψ-carotene-2,1′,2′-triol;1′,16′-Didehydro-1′,2′-dihydro-β,ψ-caroten-2′-ol;3′,4′-Didehydro-1′,2′-dihydro-β,ψ-caroten-1′-ol;3′,4′-Didehydro-1′,2′-dihydro-β,ψ-caroten-2′-ol;3,4-Didehydro-1,2-dihydro-ψ,ψ-caroten-1-ol;3′,4′-Didehydro-18′-hydroxy-γ-carotene; 7,8-Didehydroisorenieratene;3′,4′-Didehydro-4-keto-γ-carotene; 7,8-Didehydrorenieratene;4′,5′-Didehydro-4,5′-retro-β,β-carotene;4′,5′-Didehydro-4,5′-retro-β,ψ-carotene; Didehydroretro-γ-carotene;4′,5′-Didehydro-4,5′-retro-β,β-carotene-3,3′-diol;4′,5′-Didehydro-4,5′-retro-β,β-carotene-3,3′-dione;10′,11′-Didehydro-5,8,11′,12′tetrahydro-10′-apo-β-carotene-3,5,8-triol;6′,7′-Didehydro-5,6,5′,6′tetrahydro-β,β-carotene-3,5,6,3′,5′-pentol;6,7-Didehydro-5,6,5′,6′-tetra hydro-β,β-carotene-3,5,3′,5′tetrol;3,4-Didehydro-1,2,7′,8′-tetra hydro-ψ,ψ-caroten-1-ol;Didehydrotrikentriorhodin; 7,8-Didehydrozeaxanthin; Didemethylatedspirilloxanthin; 1,2,1′,2-Diepoxy-2,2′-bis(3-hydroxy-3-methylbutyl)3,4-didehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene;Diepoxy-β-carotene; 5,8,5′,8′-Diepoxycryptoxanthin;5,6,5′,6′-Diepoxy-5,6,5′,6′-tetrahydro-β,β-carotene;5,6,5′,8′-Diepoxy-5,6,5′,8′tetrahydro-β,β-carotene;5,8,5′,8′-Diepoxy-5,8,5′,8′tetrahydro-β,β-carotene;5,6,5′,6′-Diepoxy-5,6,5′,6′-tetrahydro-β,β-carotene-3,3′-diol;5,6,5′,8′-Diepoxy-5,6,5′,8′tetrahydro-β,β-carotene-3,3′-diol;5,8,5′,8′-Diepoxy-5,8,5′,8′tetrahydro-β,β-carotene-3,3′-diol;5,6,5′,6′-Diepoxy-5,6,5′,6′tetrahydro-β,β-caroten-3-ol;5,6,5′,8′-Diepoxy-5,6,5′,8′tetrahydro-β,β-caroten-3-ol;5,8,5′,8′-Diepoxy-5,8,5′,8′tetrahydro-β,β-caroten-3-ol;5,6,5′,8′-Diepoxyzeaxanthin; 5,8,5′,8′-Diepoxyzeaxanthin; Digentiobiosyl8,8′-diapocarotene-8,8′-dioate;Di-(β,D-glucopyranosyi)-4,4′-diapocarotene-4,4′-dioate; Diglucosyl8,8′-diapocarotene-8,8′-dioate; Dihydroanhydrorhodovibrin;9′,10′-Dihydro-9′-apo-β-carotene-3,9′-dione;9′,10′-Dihydro-9′-apo-ε-carotene-3,9′-dione;7′,8′-Dihydro-7′-apo-β-caroten-8′-one;5′,6′-Dihydro-5′-apo-18′-nor-β-caroten-6′-one; 7,8-Dihydroastaxanthin;β-Dihydrocarotene; 1,1′-Dihydro-carotene; 3,4-Dihydro-β-carotene;7,7′-Dihydro-β-carotene; 7′,8′-Dihydro-β,ψ-carotene;7′,8′-Dihydro-γ-carotene; 7′,8′-Dihydro-γ,ψ-carotene;7′,8′-Dihydro-6-carotene; 7′,8′-Dihydro-ε,ψ-carotene;1,2-Dihydro-ζ-carotene; 1,2-Dihydro-ψ,ψ-carotene;7,8-Dihydro-ψ,ψ-carotene; 7,8-Dihydro-β,β-carotene 3,3′-diol;7′,8′-Dihydro-β,ψ-carotene 3,17′-diol;9′,10′-Dihydro-β,ψ-carotene-3,17′-diol;7′,8′-Dihydro-ε,ψ-carotene-3,17′-diol;1,2-Dihydro-ψ,ψ-carotene-1,20-diol; 5,6-Dihydro-β,β-carotene3,5,6,3′-tetrol; 5,6-Dihydro-β,β-carotene 3,5,3′-triol;1′,2′-Dihydro-β,ψ-caroten 1′-ol; 7′,8′-Dihydro-β,ψ-caroten 3-ol;1′,2′-Dihydro-φ,ψ-caroten-1′-ol; 1,2-Dihydro-ψ,ψ-caroten-1-ol;5,6-Dihydro-β,β-carotene-3,5,6,3′-tetrol;5,6-Dihydro-β,ε-carotene-3,5,6,3′-tetrol; 7,8(or7′,8′)-Dihydrodecaprenoxanthin monoglucoside;1′,2′-Dihydro-3′,4′-dehydro-3,1′-dihydroxy-γ-carotene;1,2-Dihydro-3,4-dehydrolycopene; 1,2-Dihydro-3,4-dehydro-1-OH-lycopene;7,8-Dihydro-4,4′-diapocarotene; 7′,8′-Dihydro4,4′-diapocaroten-4-al;7′,8′-Dihydro-4,4′-diapocaroten-4-oic acid;1′,2′-Dihydro-3′,4′-didehydro-3,1′-dihydroxy-γ-caroten-2′yl rhamnoside;1′,2′-Dihydro-1′,2′dihydroxy-4-ketotorulene;1′,2′-Dihydro-3,1′-dihydroxytorulene glucoside;1′,2′-Dihydro-3,1′-dihydroxytorulene rhamnoside;1′,2′-Dihydro-4,2′-diketotorulene; 3′-Dihydro-α-doradecin;1′,2′-Dihydro-1′-glucosyl-3,4-dehydrotorulene;1′,2′-Dihydro-1′-glucosyl-4-ketotorulene;1′,2′-Dihydro-1′-hydroxy-γ-carotene;1′,2′-Dihydro-1′-hydroxychiorobactene;1′,2′-Dihydro-2′-hydroxy-3′,4′-dehydro-4-keto-γ-carotene;1′,2′-Dihydro-1′-hydroxy-3,4-dehydrotorulene glucoside;1′,2′-Dihydro-1′-hydroxy-4-keto-γ-carotene;1′,2′-Dihydro-1′-hydroxy-4-ketotorulene;1′,2′-Dihydro-1′-hydroxy-4-ketotorulene glucoside;1′,2′-Dihydro-1′-hydroxysphe roideneone;1′,2′-Dihydro-1′-hydroxytorulene glucoside;1′,2′-Dihydro-1′-hydroxytorulene rhamnoside; 1,2-Dihydrolycopene;1′,2′-Dihydrolycopene; 7,8-Dihydrolycopene;1,2-Dihydro-1-methoxy-lycopen-20-al; Dihydromethoxylycopene;5,6-Dihydro-4-methoxy-lycopen-6-one; 1,2-Dihydroneurosporene;1′,2′-Dihydroneurosporene; 1,2-Dihydro-1-OH-lycopene;1′,2′-Dihydro-1′-OH-torulene; 2′-Dihydrophillipsiaxanthin;Dihydrophytoene; 1,2-Dihydrophytoene; 1′,2′-Dihydrophytoene;1,2-Dihydrophytofluene; 1′,2′-Dihydrophytofluene;7,8-Dihydro-8,7′-retro-β,β-carotene; 7′,8′-Dihydrorhodovibrin; 7,8(or7′,8′)-Dihydrosarcinaxanthin; 3,4-Dihydrospheroidene;11′,12′-Dihydrospheroidene; 3,4-Dihydrospirilloxanthin;3,3′-Dihydroxycanthaxanthin; 3,3′-Dihydroxy-α-carotene;3,4-Dihydroxy-β-carotene; 2,3-Dihydroxy-β,β-carotene-4,4′-dione;3,3′-Dihydroxy-ε-carotene; 2,3′-Dihydroxy-β,β-carotene-4,4′-dione;3,3′-Dihydroxy-β,β-carotene-4,4′-dione;3,3′-Dihydroxy-β,ε-carotene-4,2′-dione;3,3′-Dihydroxy-β,χ-carotene-4,6′-dione;3,3′-Dihydroxy-χ,χ-carotene-6,6′-dione; 2,3-Dihydroxy-β,β-caroten-4-one;3,3′-Dihydroxy-β,β-caroten-4-one; 3,2′-Dihydroxy-β,ε-caroten-4-one;3,3′-Dihydroxy-β,ε-caroten-4-one; 3,3′-Dihydroxy-β,χ-caroten-6′-one;3,8-Dihydroxy-χ,X-caroten-6-one; 3,3′-Dihydroxydehydro-β-carotene;3,3′-Dihydroxy-7,8-dehydro-β-carotene;3,3′-Dihydroxy-7,8,7′,8′-dehydro-β-carotene;3,3′-Dihydroxy-7,8-dehydro-β-carotene-5′,6′-epoxide;3,3′-Dihydroxy-2,3-didehydro-β,β-carotene-4,4′-dione;3,3′-Dihydroxy-7,8-didehydro-β,β-carotene-4,4′-dione;3′,8′-Dihydroxy-7,8-didehydro-β,χ-carotene-3′,6′-dione;3,3′-Dihydroxy-2,3-didehydro-β,β-caroten-4-one;3,3′-Dihydroxy-7′,8′-didehydro-β,β-caroten-4-one;3,4′-Dihydroxy-2,3-didehydro-β,β-caroten-4-one;3,3′-Dihydroxy-2,3-didehydro-β,ε-caroten-4-one;3,8-Dihydroxy-7′,8′-didehydro-χ,X-caroten-6-one;3,6′-Dihydroxy-7,8-didehydro-6′,7′dihydro-β,ε-carotene-3′,8′-dione;3,3′-Dihydroxy-7,8-didehydro-7′,8′dihydro-β,χ-carotene-6′,8′-dione;3,1′-Dihydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;1′,2′-Dihydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;3,5-Dihydroxy-6,7-didehydro-5,6,7′,8′-tetrahydro-7′-apo-β-caroten-8′-one;6,3′-Dihydroxy-7′,8′-didehydro-5,6,7,8-tetrahydro-β,β-carotene-3,8-dione;3,3′-Dihydroxy-5,8,5′,8′-diepoxy-β-carotene;5,6-Dihydroxy-5,6-dihydro-10′-apo-β-caroten-10′-al;5,6-Dihydroxy-5,6-dihydro-10′-apo-β-caroten-10′-oic acid;5,6-Dihydroxy-5,6-dihydro 12′-apo-β-caroten-12′-oic acid;3,3′-Dihydroxy-7,8-dihydro-β,β-carotene-4,4′-dione;3,1′-Dihydroxy-1′,2′-dihydrotorulene;1′,2′-Dihydroxy-1′,2′-dihydrotorulene;3,3′-Dihydroxy-4,4′-diketo-β-carotene;3,3′-Dihydroxy-2,2′-dinor-β,β-carotene-4,4′-dione-3,3′-diacylate;3,19-Dihydroxy-3′,6′-dioxo-7,8-didehyro-β,χ-caroten-17-al;1,1′-Dihydroxy-2,2′-dirhamnosyl-1,2,1′,2′-tetrahydro-3,4,3′,4′-tetrahydrolycopene;3,3′-Dihydroxyechinenone; 3,3′-Dihydroxy-5,6-epoxy-αcarotene;3,3′-Dihydroxy-5,8-epoxy-α-carotene;3,3′-Dihydroxy-5,6-epoxy-β-carotene;3,3′-Dihydroxy-5,8-epoxy-β-carotene;2-(Dihydroxyisopentenyl)-2′-isopentenyl-β-carotene;3,3′-Dihydroxyisorenieratene; 3,3′-Dihydroxy-4-keto-gcarotene;3,3′-Dihydroxyluteochrome; Dihydroxylycopene;3,1′-Dihydroxy-2′-(5-C-methylpentosyloxy)-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;Dihydroxyneurosporene; 2′,3′-Dihydroxy-2-nor-β,β-carotene-3,4-dione;3,3′-Dihydroxy-2-nor-13-β,β-carotene-4,4′-dione-3-acylate;3,3′-Dihydroxy-2-nor-13-β,β-carotene-4,4′-dione-3,3′-di-acylate;1,2-Dihydroxyphytofluene; Dihydroxypirardixanthin;3,3′-Dihydroxyretro-β-carotene;3,3′-Dihydroxy-2,3,2′,3′-tetradehydro-β,β-carotene-4,4′-dione;3,3′-Dihydroxy-7,8,7′,8′-tetradehydro-β,β-carotene-4,4′-dione;3,3′-Dihydroxy-2,3,2′,3′-teradehydro-β,β-carotene-4,4′-dionedipalmitate; 3,3′-Dihydroxy-7,8,7′,8′-tetradehydro-β,β-caroten-4-one;1,1′-Dihydroxy-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydrod-ψ,ψ-carotene-2,2′-dione;3,8′-Dihydroxy-5′,6′,7′,8′-tetrahydro-5′-apo-18′-nor-β-caroten-6′-one;1,1′-Dihydroxy-1,2,1′,2′-tetrahydro-ζ-carotene;5,5′-Dihydroxy-5,6,5′,6′-tetrahydro-β,β-carotene-3,3′-dione;3,3′-Dihydroxy-7,8,7′,8′-tetrahydro-χ,χ-carotene-6,6′-dione;9′,10′-Dihydro-β-zeacarotene 3,17′-iol; Diketo-, see also Dioxo- or-dione 2,2′-Diketobacterioruberin; 3,4-Diketo-β-carotene;4,4′-Diketo-β-carotene; 4,4′-Diketo-γ-carotene;4,4′-Diketocynthiaxanthin; 3,3′-Diketodehydro-β-carotene;4,4′-Diketolycopene; Diketopirardixanthin; 3,3′-Diketoretro-β-carotene;3,3′-Diketoretrodehydro-β-carotene; 2,2′-Diketospirilloxanthin;4,4′-Diketo-7,8,7′,8′-tetrade hydrozeaxanthin;3,3′-Dimethoxy-β,β-carotene; 3,3′-Dimethoxy-β,ε-carotene;3,3′-Dimethoxy-γ-carotene; 3,3′-Dimethoxy-3′,4′-dehydro-γ-carotene;1,1′-Dimethoxy-3,4-didehydro-1,2,1′,2′,7′,8′-hexahydro-ψ,ψ-carotene;1,1′-Dimethoxy-3,4-didehydro-1,2,1′,2′,7′,8′-hexahydro-ψ,ψ-caroten-2-one;1,1′-Dimethoxy-3,4-didehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene;1,1′-Dimethoxy-3′,4′-didehydro-1,2,1′,2′-tetrahydro-ψ,ψ-caroten-4-one;1,1′-Dimethoxy-1,2,7,8,1′,2′-hexahydro-ψ,ψ-carotene;1,1′-Dimethoxy-1,2,7,8,11,12,1′,2′-octahydro-ψ,ψ-carotene;1,1′-Dimethoxy-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene;1,1′-Dimethoxy-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene-2,2′-dione;1,1′-Dimethoxy-1,2,1′,2′-tetrahydro-ψ,ψ-caroten-20-al;1,1′-Dimethoxy-1,2,1′,2′-tetrahydro-ψ,ψ-carotene;1,1′-Dimethoxy-1,2,1′,2′-tetrahydro-ψ,ψ-carotene-4,4′-dione;1,1ψ-Dimethoxy-1,2,1′,2′-tetrahydrolycopene;1,1′-Dimethoxy-1,1′,2,2′-tetrahydroneurosporene; Dimethylcrocetin;Dimethyl-6,6′-diapocarotene-6,6′-dioate;Dimethyl-8,8′-diapocarotene-8,8′-dioate;Dineapolitanosyl-8,8′-diapocarotene-8,8′-dioate;2,2′-Dinor-β,β-carotene-3,4,31,4′tetrone; Dinoxanthin;3,3′-Dioxi-4-oxo-β-carotene; Dioxo-, see also Diketo- or -dione5,6-Dioxo-10′-apo-5,6-seco-β-caroten-10′-al;5,6,5′,6′-Diseco-β,β-carotene 5,6,5′,6′-tetrone;7,8,11,12,13,14,15,7′,8′,11′,12′,15′-Dodecahydro-13,15′:14,15′biscyclo-15,15′-seco-ψ,ψ-caroten-15-ol; Dodecahydrolycopene;α-Doradecin; β-Doradecin; α-Doradexanthin; β-Doradexanthin;

Echinenone; Echininone; Eloxanthin; 6-Epikarpoxanthin; 3′-Epilutein;5,6-Epoxy-α-carotene; 5,8-Epoxy-α-carotene; 5,8-Epoxy-β-carotene;1,2-Epoxy-1,2,7,8,11,12,7′,8′,11′,12′-decahydro-ψ,ψ-carotene;5,6-Epoxy-7′,8′-didehydro-5,6-dihydro-β,β-carotene-3,3′-diol;5,8-Epoxy-7′,8′-didehydro-5,8-dihydro-β,β-carotene-3,3′-diol;1′,2′-Epoxy-3′,4′-didehydro-1,2′-dihydro-β,ψ-caroten-2-ol;5′,6′-Epoxy-6,7-didehydro-5,6,5′,6′-tetrahydro-β,β-carotene-3,5,19(or19′),3′-tetrol;5′,6′-Epoxy-6,7-didehydro-5,6,5′,6′-tetrahydro-β,β-carotene-3,5,3′-triol;5′,6′-Epoxy-6,7-didehydro-5,6,5′,6′-tetrahydro-β,β-carotene-3,5,3′-triol3-acetate;5′,8′-Epoxy-6,7-didehydro-5,6,5′,8′-tetrahydro-β,β-carotene-3,5,3′-triol;5,6-Epoxy-5,6-dihydro-12′-apo-β-carotene-3,12′-diol;5,8-Epoxy-5,8-dihydro-10′-apo-β-carotene-3,10′-diol;5,8-Epoxy-5,8-dihydro-12′-apo-β-carotene-3,12′-diol;5,6-Epoxy-5,6-dihydro-β,β-carotene; 5,8-Epoxy-5,8-dihydro-β,β-carotene;5,6-Epoxy-5,6-dihydro-β,ε-Ecarotene; 5,8-Epoxy-5,8-dihydro-β,ε-carotene;1′,2′-Epoxy-1′,2′-dihydro-β,ψ-carotene;1′,2′-Epoxy-1′,2′-dihydro-ε,ψ-carotene;1,2-Epoxy-1,2-dihydro-ψ,ψ-carotene; 5,6-Epoxy-5,6-dihydro-ψ,ψ-carotene;5,6-Epoxy-5,6-dihydro-β,β-carotene-3,3′-diol;5,8-Epoxy-5,8-dihydro-β,β-carotene-3,3′-diol;5,6-Epoxy-5,6-dihydro-β,ε-carotene-3,3′-diol;5,6-Epoxy-5,6-dihydro-β,ε-carotene-3,3′-diol dipalmitate;5,8-Epoxy-5,8-dihydro-β,ε-carotene-3,3′-diol;5,6-Epoxy-5,6-dihydro-β,ε-carotene-3,3′,6′-triol;5,8-Epoxy-5,8-dihydro-β,ε-carotene-3,3′,6′-triol;5,6-Epoxy-5,6-dihydro-β,β-caroten-2-ol;5,6-Epoxy-5,6-dihydro-β,β-caroten-3-ol;5′,8′-Epoxy-5′,8′-dihydro-β,β-caroten-3-ol;5,6-Epoxy-5,6-dihydro-β,ε-caroten-2-ol;5,6-Epoxy-5,6-dihydro-β,ψ-caroten-3-ol;5,8-Epoxy-5,8-dihydro-β,ψ-caroten-3-ol;5,8-Epoxy-3,3′-dihydroxy-α-carotene;5,6-Epoxy-3,3′-dihydroxy-7′,8′didehydro-5,6,7,8-tetrahydrod-β,β-caroten-8-one;5′,6′-Epoxy-3,3′-dihydroxy-7,8-didehydro-5′,6′-dihydro-10,11,20-trinor-β,β-caroten-19′,11′-olide;5′,6′-Epoxy-3,3′-dihydroxy-4,7-didehydro-5′,6′-dihydro-10,11,20-trinor-β,β-caroten-19′,11′-olide3-acetate;5′,6′-Epoxy-3,3′-dihydroxy-7,8-didehydro-5′,6′-dihydro-10,11,20-trinor-β,β-caroten-19′,11′-olide3-acetate; 5,6-Epoxy-3,3′-dihydroxy-5,6-dihydro-β,ψ-caroten-6′-one;5,8-Epoxy-3,3′-dihydroxy-5,8-dihydro-β,ψ-caroten-6′-one;5,6-Epoxy-3,3′-dihydroxy-5,6,7′,8′-tetrahydro-β,ε-caroten-11′,19′-olide;1′,2′-Epoxy-2′-(2,3-epoxy-3-methylbutyl)-2-(3-hydroxy-3-methylbutyl)-3′,4′-didehydro-1,2,1′,2′-tetrahydro-ψ,ψ-caroten-1-ol;1,2-Epoxy-1,2,7,8,7′,8′-hexahydro-ψ,ψ-carotene;5,6-Epoxy-3-hydroxy-8′-apo-β-caroten-8′-al;5,6-Epoxy-5,6-dihydro-10′-apo-β-carotene-3,10′-diol;5,8-Epoxy-3-hydroxy-γ-carotene;5,8-Epoxy-3-hydroxy-5,8-dihydro-8′-apo-β-caroten-8′-al;5,6-Epoxy-3-hydroxy-5,6-dihydro-10′-apo-β-caroten-10′-al 502;5,6-Epoxy-3-hydroxy-5,6-dihydro-12′-apo-β-caroten-12′-al;5,6-Epoxy-3-hydroxy-5,6,7′,8′-tetrahydro-7′-apo-β-caroten-8′-one;5,8-Epoxylutein; 1,2-Epoxy-1,2,7,8,11,12,7′,8 ′octahydro-ψ,ψ-carotene;1,2-Epoxy-1,2,7,8,7′,8′,11′,12′octahydro-ψ,ψ-carotene;1′,2′-Epoxy-7,8,11,12,1′,2′,7′,8′-octahydro-β,ψ-caroten-2-ol;1,2-Epoxyphytoene; 5,8-Epoxyrubixanthin;5′,8′-Epoxy-5,6,5′,8′-tetrahydro-β,β-carotene-3,5,6,3′-tetrol;5′,6′-Epoxy-5,6,5′,6′-tetrahydro-β,β-carotene-3,5,6,3′-tetrol;5,6-Epoxy-3′,4′,7′,8′-tetradehydro-5,6-dihydro-β,β-caroten-4-one;5,6-Epoxy-3,3′,5′,19′-tetra-hydroxy-6′,7′-didehydro-5,6,7,8,5′,6′-hexahydro-β,β-caroten-8-one3′-acetate 19′-hexanoate;5,6-Epoxy-3,3′,5′-trihydroxy-6′,7′-didehydro-5,6,7,8,5′,6′-hexahydro-β,β-caroten-8-one;5,6-Epoxy-3,3′,5′-trihydroxy-6′,7′-didehydro-5,6,7,8,5′,6′-hexahydro-β,β-caroten-8-one3′-acetate;5′,6′-Epoxy-3,5,3′-trihydroxy-6,7-didehydro-5,6,5′,6′-tetrahydro-10,11,20-trinor-β,β-caroten-19′,11′-olide;5′,6′-Epoxy-3,5,3′-trihydroxy-6,7-didehydro-5,6,5′,6′-tetrahydro-10,11,20-trinor-β,β-caroten-19′,11′-olide3-acetate;4′,5′-Epoxy-3,6,3′-trihydroxy-7,8,4′,5′,7′,8′-hexahydro-γ,ε-caroten-8-one;5,6-Epoxyzeaxanthin; 5,8-Epoxyzeaxanthin; Eschscholtzxanthin;Eschscholtzxanthone; 4′-Ethoxy-β,β-caroten-4-one;4′-Ethoxy-4-keto-β-carotene; Euglenanone; Euglenarhodon;Eutreptiellanone;

Flavacin; Flavochrome; Flavorhodin; Flavoxanthin; Flexixanthin;Foliachrome; Foliaxanthin; Fritschiellaxanthin; Fucocirome; Fucoxanthin;Fucoxanthinol; Fucoxanthol;

Gazaniaxanthin; β,D-Gentiobiosyl β,D-glucosyl8,8′-diapocarotene-8,8′-dioate; Gentiobiosyl hydrogen-8,8′-dioate;Gentiobiosyl neapolitanosyl 8,8′-diapocarotene-8,8′-dioate; β,D-GIucosylhydrogen-4,4′-diapocarotene-4,4′-dioate; 4′-β,D-Glucosyl4-hydrogen-7′,8′-dihydro-4,4′-diapocarotene-4,4′-dioate; β,D-Glucosylhydrogen-8,8′-diapocarotene-8,8′-dioate; β,D-Glucosylmethyl-8,8′-diapo-carotene-8,8′-dioate; Glucopyranosyloxy (seeGlucosyloxy); 4-Glucosyloxy-4,4′-diaponeurosporene;1′-Glucosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-carotene;1-Glucosyloxy-3,4-didehydro-1,2-dihydro-ψ,ψ-carotene;

2′-Glucosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-carotene-3,1′-dio;1′-Glucosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-3-ol;1′-Glucosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-2′-ol;1′-Glucosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;1-Glucosyloxy-3,4-didehydro-1,2,7′,8′-tetrahydro-ψ,ψ-carotene;1-Glucosyloxy-1,2-dihydro-ψ,ψ-caroten-20-al;1-Glucosyloxy-1′,2′-dihydro-β,ψ-carotene;1′-Glucosyloxy-1′,2′-dihydro-φ,ψ-carotene;1-Glucosyloxy-1,2-dihydro-ψ,ψ-carotene;4-Glucosyloxy-7′,8′-dihydro-4,4′-diapocarotene;1′-Glucosyloxy-2′-hydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;2-(4-Glucosyloxy-3-methyl-2-butenyl)-2′-(4-hydroxy-3-methyl-2-butenyl)-γ,γ-carotene;2-(4-Glucosyloxy-3-methyl-2-butenyl)-2′-(4-hydroxy-3-methyl-2-butenyl)-ε,ε-carotene;2-(4-Glucosyloxy-3-methyl-2-butenyl)-2′-(4-hydroxy-3-methyl-2-butenyl)-7,8-dihydro-ε,ε-carotene;2′-(4-Glucosyloxy-3-methyl-2-butenyl)-2-(3-methyl-2-butenyl)-ε,ε-caroten-18-ol;2-[3-(Glucosyloxy)-3-methylbutyl]-2′-(3-hydroxy-3-methylbutyl)-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene-1,1′-diol;1′-Glucosyloxy-3,4,3′,4′-tetradehydro-1′,2′-dihydro-β,ψ-carotene;Glycymerin; Guaraxanthin;

Halocynthiaxanthin; Helenien; Heteroxanthin; Hexadecahydrolycopene;2,3,2′,3′,4′,5′-Hexadehydro-4,5′-retro-β,β-carotene;1,2,7,8,11,12-Hexahydro-ψ,ψ-carotene;1,2,7,8,1′,2′-Hexahydro-ψ,ψ-carotene;1,2,7,8,7′,8′-Hexahydro-ψ,ψ-carotene;7,8,11,12,7′,8′-Hexahydro-ψ,ψ-carotene;7,8,11,12,7′,8′-Hexahydro-β,ψ-caroten-2-ol;15,7′,8′,11′,12′,15′-Hexahydro-β,ψ-caroten-2-ol;1,2,7′,8′,11′,12′-Hexahydro-ψ,ψ-caroten-1-ol;7,8,11,12,7′,8′-Hexahydro-ψ,ψ-caroten-16-ol;7,8,11,12,7′,8′-Hexahydro-4,4′-diapocarotene;1,2,7,8,11,12-Hexahydrolycopene; 1′,2′,7′,8′11′,12′-Hexahydrolycopene;7,8,11,12,7′,8′-Hexahydrolycopene; 7,8,1′,2′,7′,8′-Hexahydrolycopene;3,4,3′,4′,7′,8′-Hexahydrospirilloxanthin; 19′-Hexanoyloxyfucoxanthin;19-Hexanoyloxyparacentrone;1-Hexosyl-1,2-dihydro-3,4-didehydroapo-8′-lycopenol;O-Hexosyl-1′-hydroxy-1′,2′-dihydro-γ-carotene;O-Hexosy-1-4-keto-1′-hydroxy-1′,2′-dihydro-3′,4′-didehydro-γ-carotene;Hopkinsiaxanthin; Hydroxy-, see also Monohydroxy-, OH or-ol3-Hydroxy-β-apo-2-carotenal; 3-Hydroxy-8′-apo-β-caroten-8′-al;3-Hydroxy-10′-apo-β-caroten-10′-al; 3-Hydroxy-12′-apo-β-caroten-12′-al;3-Hydroxy-8′-apo-ε-caroten-8′-al; 3-Hydroxy-8′-apo-β-caroten-8′-oicacid; 9′-Hydroxy-9′-apo-β-caroten-3-one;9′-Hydroxy-9′-apo-ε-caroten-3-one; Hydroxyasteroidenone;3-Hydroxycanthaxanthin; 3-Hydroxy-β,ψ-caroten-18′-al;3-Hydroxy-α-carotene; 3′-Hydroxy-α-carotene; 4-Hydroxy-α-carotene;2-Hydroxy-β-carotene; 3-Hydroxy-β-carotene; 4-Hydroxy-β-carotene;3-Hydroxy-γ-carotene; 4′-Hydroxy-γ-carotene; 3-Hydroxy-δ-carotene;2-Hydroxy-β,β-carotene-4,4′-dione; 3-Hydroxy-β,β-carotene-4,4′-dione;3′-Hydroxy-β,β-carotene-3,4-dione; 4′-Hydroxy-β,β-carotene-3,4-dione;3-Hydroxy-β,ε-carotene-4,3′-dione; 3′-Hydroxy-β,ε-carotene-3,4-dione;3-Hydroxy-β,χ-carotene-3′,6′-dione;3′-Hydroxy-β,β-carotene-3,4,4′-trione; 2′-Hydroxy-β,β-caroten-2-one;2-Hydroxy-β,β-caroten-4-one; 3-Hydroxy-β,β-caroten-4-one;3′-Hydroxy-β,β-caroten-4-one; 4′-Hydroxy-β,β-caroten-4-one;3-Hydroxy-β,ε-caroten-4-one; 3-Hydroxy-β,ε-caroten-3′-one;3′-Hydroxy-β,χ-caroten-6′-one; 3-Hydroxy-β,ψ-caroten-4′-one;3-Hydroxy-β,ψ-caroten-4-one; 3-Hydroxy-ε,ε-caroten-3′-one;3′-Hydroxy-ψ,ψ-caroten-4-one; 3-Hydroxycitranaxanthin;3-Hydroxy-7,8-dehydro-α-carotene; 3′-Hydroxy-3,4-dehydro-β-carotene;3-Hydroxy-3′,4′-dehydro-γ-carotene; 4-Hydroxy-4,4′-diaponeurosporene;3-Hydroxy-2,3-didehydro-β,β-carotene-4,4′-dione;2′-Hydroxy-3,4-didehydro-β,β-caroten-2-one;3-Hydroxy-2,3-didehydro-β,β-caroten-4-one;3-Hydroxy-2,3-didehydro-β,ε-caroten-4-one;3-Hydroxy-2,3-didehydro-β,χ-caroten-4-one;3-Hydroxy-2,3-didehydro-β,φ-caroten-4-one;3-Hydroxy-3′,4′-didehydro-β,ψ-caroten-4-one;3-Hydroxy-7,8-didehydro-7′,8′-dihydro-7′-apo-β-carotene-4,8′-dione;3-Hydroxy-7,8-didehydro-7′,8′-dihydro-7′-apo-β-caroten-8′-one;3-Hydroxy-7′,8′-didehydro-7,8-dihydro-χ,X-carotene-6,8-dione;1′-Hydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;1′-Hydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-2′-one;2′-Hydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;5-Hydroxy-4′,5′-didehydro-4,5-dihydro-4,5′-retro-β,β-carotene-3,3′-dione;3′-Hydroxy-2′,3′-didehydro-2-nor-β,β-carotene-3,4,4′-trione;3′-Hydroxy-4′,5′-didehydro-4,5′-retro-β,β-caroten-3-one;3-Hydroxy-5,8,5′,8′-diepoxy-β-carotene;3-Hydroxy-7′,8′-dihydro-7′-apo-β-caroten-8′-one;3-Hydroxy-5′,6′-dihydro-5′-apo-18′-nor-β-caroten-6′-one;1-Hydroxy-1,2-dihydro-ψ,ψ-caroten-20-al;1′-Hydroxy-1′,2′-dihydro-γ-carotene;3-Hydroxy-7,8-dihydro-χ,X-carotene-6,8-dione;4′-Hydroxy-5′,6′-dihydro-β,β-caroten-4-one;1′-Hydroxy-1′,2′-dihydro-β,ψ-caroten-4-one;8′-Hydroxy-7′,8′-dihydrocitranaxanthin;4-Hydroxy-7′,8′-dihydro-4,4′-diapocarotene;4′-Hydroxy-5′,6′-dihydroechinenone;1′-Hydroxy-1′,2′-dihydro-2-isopentenyl-2′-(hydroxyisopentenyl)torulene;1-Hydroxy-1,2-dihydrolycopene; 1-Hydroxy-1,2-dihydroneurosporene;1′-Hydroxy-1′,2′-dihydroneurosporene; 1-Hydroxy-1,2-dihydrophytoene;1(or 1′)-Hydroxy-1,2 (or 1′,2′)-dihydrophytofluene;8′-Hydroxy-7′,8′-dihydroreticulataxanthin;1′-Hydroxy-1′,2′-dihydrospheroidene; 2′-Hydroxy-1′,2′-dihydrotorulene;2-Hydroxy-1′,2′-dihydrotorulene-1′,2′-oxide;5-Hydroxy-5,6-dihydrozeaxanthin; 3-Hydroxy-3′,4′-diketo-α-carotene;3-Hydroxy-4,4′-diketo-β-carotene; 3′-Hydroxy-3,4-diketo-β-carotene;2′-Hydroxy-3,1′-dimethoxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;4-Hydroxy-3′,4′-dioxo-β-carotene; 2-Hydroxyechinenone;3-Hydroxyechinenone; 3′-Hydroxyechinenone; 4′-Hydroxyechinenone;3-Hydroxy-5,8-epoxy-β-carotene;3′-Hydroxy-3,6-epoxy-5,6-dihydro-β,ε-caroten-4-one;3′-Hydroxy-3,6-epoxy-7′,8′-didehydro-5,6-dihydro-β,β-caroten-4-one;3′-Hydroxyeuglenanone; 2′-Hydroxyflexixanthin;1-Hydroxy-1,2,7′,8′,11′,12′-hexahydrolycopene;1′-Hydroxy-3,4,1′,2′,11′,12′hexahydrospheroidene;2-(4-Hydroxy-3-hydroxymethyl-2-butenyl)-2′-(3-methyl-2-butenyl)-β,β-carotene;3-Hydroxyisorenieratene; 3-Hydroxy-4-keto-α-carotene;3-Hydroxy-3′-keto-α-carotene; 3-Hydroxy-4-keto-β-carotene;3-Hydroxy-4′-keto-β-carotene; 4-Hydroxy-4′-keto-β-carotene;1′-Hydroxy-2′-keto-1′,2′-dihydrotorulene;3-Hydroxy-3′-keto-retrodehydrocarotene; 19-Hydroxylutein;16-Hydroxylycopene; 3-Hydroxy-3′-methoxy-β-carotene;1′-Hydroxy-1-methoxy-3,4-didehydro-1,2,1′,2′,7′,8′-hexahydro-ψ,ψ-caroten-2-one;1′-Hydroxy-1-methoxy-1,2,1′,2′,7′,8′-hexahydro-ψ,ψ-caroten-4-one;1′-Hydroxy-1-methoxy-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-caroten-2-one;1′-Hydroxy-1-methoxy-1,2,1′,2′-tetrahydro-ψ,ψ-caroten-4-one;2-(4-Hydroxy-3-methyl-2-butenyl)-β,β-carotene;2-(4-Hydroxy-3-methyl-2-butenyl)-ε,ψ-carotene;2-(3-Hydroxymethyl-but-2-enyl)-7′,8′-dihydro-δ-carotene;2-(4-Hydroxy-3-methyl-2-butenyl)-7′,8′-dihydro-ε,ψ-carotene;2-(4-Hydroxy-3-methyl-2-butenyl)-2′-(3-methyl-2-butenyl)-ε,ε-carotene;2-(4-Hydroxy-3-methyl-2-butenyl)-2′-(3-methyl-2-butenyl)-ε,ε-caroten-18-ol;2′-(4-Hydroxy-3-methyl-2-butenyt)-2-(3-methyl-2-butenyl)-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-1′-ol;2(or 2′)-(4-Hydroxy-3-methyl-2-butenyl)-2′(or2)-(3-methyl-2-butenyl)-3′,4′-didehydro-1′,2′-dihydro-ε,ψ-caroten-1′-ol;2′(4-Hydroxy-3-methyl-2-butenyl)-2-(3-methyl-2-butenyl)-7,8(or7′,8′)-dihydro-ε,ε-caroten-18-ol;2-(4-Hydroxy-3-methyl-2-butenyl)-7,8,7′,8′-tetrahydro-ε,ψ-carotene;2-(4-Hydroxy-3-methyl-2-butenyl)-7′,8′,11′,12′-tetrahydro-ε,ψ-carotene;16-(3-Hydroxy-3-methylbutyl)-16′(3-methyl-2-butenyl)-7,8,11,12,15,7′,8′,11′,12′,15′-decahydro-ψ,ψ-carotene;2-(3-Hydroxy-3-methylbutyl)-2′-(3-methyl-2-butenyl)-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene-1,1′-diol;2-Hydroxy-monocyclic-phytofluene; 4-Hydroxymyxoxanthophyll;Hydroxyneurosporene;15-Hydroxy-7′,8′,9′,10′,11′,12′,13′,14′-octahydro-6′-apo-β-caroten-7′-one;1′-Hydroxy-3,4,7,8,1′,2′,11′,12′-octahydrospheroidene;3′-Hydroxy-4-oxo-β-carotene; 3-Hydroxy-4-oxo-2,3-dehydro-4-carotene;4′-Hydroxy-3-oxoechinenone; Hydroxyphytoene; Hydroxyphytofluene;4′-Hydroxy-4-oxo-pirardixanthin; 2-Hydroxyplectaniaxanthin;3-Hydroxy-4,5′-retro-5′-apo-β-caroten-5′-one;3-Hydroxy-4′,12′-retro-β,β-carotene-3′,12′-dione; 3′-Hydroxyrubixanthin;3′-Hydroxy-5,6-seco-β,β-carotene-5,6-dione; 3-Hydroxysemi-β-carotenone;3-Hydroxysintaxanthin; Hydroxyspheroidene; Hydroxyspheroidenone;Hydroxyspirilloxanthin;8′-Hydroxy-5′,6′,7′,8′-tetrahydro-5′-apo-18′-nor-β-caroten-6′-one;4′-Hydroxy-5,6,5′,6′-tetrahydro-β,β-caroten-4-one;1-Hydroxy-3,4,3′,4′-tetradehydro-1,2-dihydro-ψ,ψ-caroten-2-one;1-Hydroxy-1,2,7′,8′-tetrahydrolycopene;1′-Hydroxy-3,4,1′,2′-tetrahydrospheroidene; 3-Hydroxytorulene;16′-Hydroxytorulene; 18′-Hydroxytorulene;3-Hydroxy-3′,4,4′-triketo-β-carotene; 3-Hydroxy-β-zeacarotene;5-Hydroxyzeaxanthin;

Idoxanthin; Isoagelaxanthin A; Isobixin; Isocarotene; Iso-ζ-carotene;Iso-ξ-carotene; Isocrocetin; Isocryptoxanthin; Isofucoxanthin;Isofucoxanthinol; Isolutein; Isomethylbixin; Isomytiloxanthin;2-Isopentenyl-3,4-dehydrorhodopin; Isorenieratene; β-Isorenieratene;3,3′-Isorenieratenediol; 3-Isorenieratenol; Isotedaniaxanthin;Isotedanin; Isozeaxanthin;

Karpoxanthin; Keto-, see also oxo or -one Ketocapsanthin;4-Ketocapsanthin; 4-Keto-α-carotene; 4-Keto-β-carotene;4-Keto-γ-carotene; 4-Ketocynthiaxanthin;4-Keto-3′,4′-dehydro-β-carotene;4-Keto-1′,2′-dihydro-1′-hydroxytorulene;2-Keto-7′,8′-dihydrorhodovibrin; 4-Keto-3,3′-dihydroxy-α-carotene;4′-Keto-3-hydroxy-γ-carotene; 4-Keto-3′-hydroxylycopene; 4-Ketolutein332 4-Ketomyxol 2′-(methylpentoside); 4-Ketomyxoxanthophyll;2-Keto-OH-spirilloxanthin; 4-Ketophleixanthophyll; 2-Ketorhodovibrin;4′-Ketorubixanthin; 2-Ketospirilloxanthin; 4-Ketotorulene;4-Ketozeaxanthin;

Lactucaxanthin; Latochrome; Latoxanthin; LUprotene; Lilixanthin;Loniceraxanthin; Loroxanthin; Lusomycin; Lutein; Lutein dimethyl ether;Lutein dipalmitate; Lutein epoxide; Luteochrome; Luteol; Luteoxanthin;Lycopenal; Lycopen-20-al; Lycopene; Lycopene-16,16′-diol; Lycopene1,2-epoxide; Lycopene 5,6-epoxide; Lycopen-16-ol; Lycopen-20-ol;Lycopersene; Lycophyll; Lycoxanthin;

Mactraxanthin; Manixanthin;1-Mannosyloxy-3,4-didehydro-1,2-dihydro-8′-apo-ψ-caroten-8′-ol;3′-Methoxy-β,β-caroten-3-ol; 3-Methoxy-β,X-carotene;1-Methoxy-1,2,7,8,11,12,7′,8′,11′,12′-decahydro-ψ,ψ-carotene;1′-Methoxy-1,2,7,8,11,12,1′,2′,7′,8′-decahydro-ψ,ψ-caroten-1-ol;1-Methoxy-3,4-didehydro-1,2-dihydro-ψ,ψ-caroten-20-al;1′-Methoxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-carotene;1-Methoxy-3,4-didehydro-1,2-dihydro-ψ,ψ-carotene;1-Methoxy-3,4-didehydro-1,2,7′,8′,11′,12′-hexahydro-ψ,ψ-carotene;1′-Methoxy-3′,4′-didehydro-1,2,7,8,1′,2′-hexahydro-ψ,ψ-caroten-1-ol;1-Methoxy-3,4-didehydro-1,2,7′,8′-tetrahydro-ψ,ψ-carotene;1′-Methoxy-3′,4′-didehydro-1,2,1′,2′-tetrahydro-ψ,ψ-caroten-1-ol;1-Methoxy-3,4-didehydro-1,2,7′,8′-tetrahydro-ψ,ψ-caroten-2-one;1-Methoxy-1,2-dihydro-ψ,ψ-caroten-20-al;1-Methoxy-1,2-dihydro-ψ,ψ-carotene;1′-Methoxy-1′,2′-dihydro-β,ψ-caroten-4′-one;1′-Methoxy-1′,2′-dihydro-X,ψ-caroten-4′-one;1-Methoxy-1,2-dihydro-ψ,ψ-caroten-4-one;1′-Methoxy-1′,2′-dihydro-3′,4′-dehydro-γ-carotene;1-Methoxy-1,2-dihydro-3,4-dehydrolycopene;1-Methoxy-1,2-dihydro-3,4-didehydrolycopen-20-al;1-Methoxy-1,2-dihydrolycopene; 4-Methoxy-5,6-dihydrolycopene;1-Methoxy-1,2-dihydroneurosporene; 1-Methoxy-1,2-dihydrophytoene;1-Methoxy-1,2-dihydrophytofluene; 1′-Methoxy-1′,2′-dihydrospheroidene;3-Methoxy-19,3′-dihydroxy-7,8-didehydro-β,χ-carotene-6′,8′-dione;1-Methoxy-1,2,7′,8′,11′,12′-hexahydro-ψ,ψ-carotene;1′-Methoxy-1,2,7,8,1′,2′-hexahydro-ψ,ψ-caroten-1-ol;1-Methoxy-1,2,7′,8′11′,12′-hexahydro-ψ,ψ-caroten-4-one;1-Methoxy-1′-hydroxy-1,2,1′,2′-tetrahydrophytofluene;1-Methoxy-2-keto-7′,8′-dihydro-3,4-dehydrolycopene; Methoxylycopenal;1-Methoxy-1,2,7,8,7′,8′,11′,12′-octahydro-ψ,ψ-carotene;1′-Methoxy-1,2,7,8,11,12,1′,2′-octahydro-ψ,ψ-caroten-1-ol;1-Methoxy-4-oxo-1,2-dihydro-8′-apo-ψ-caroten-8′-al;1-Methoxy-4-oxo-1,2-dihydro-12′-apo-ψ-caroten-12′-al; Methoxyphytoene;Methoxyphytofluene; Methoxyspheroidene;1′-Methoxy-3,4,3′,4′-teradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-caroten-1-ol;1-Methoxy-1,2,7′,8′-tetrahydro-ψ,ψ-carotene;1-Methoxy-1,2,7′,8′-tetrahydro-ψ,ψ-caroten-4-one;1-Methoxy-1,2,7′,8′-tetrahydro-3,4-dehydrolycopene;3Methoxy-19,3′,8′-trihydroxy-7,8-didehydro-β,χ-caroten-6′-one; Methyl4′-apo-β-caroten-4′-oate; Methyl 8′-apo-β-caroten-8′-oate; Methyl6′-apo-ψ-caroten-6′-oate; Methyl apo-6′-lycopenoate; Methylbixin;2-(3-Methyl-2-butenyl)-β,β-caroten-18(or 18′)-ol;2-(3-Methyl-2-butenyl)-3,4-didehydro-1,2-dihydro-ψ,ψ-caroten-1-ol;2-(3-Methyl-2-butenyl)-7,8,7,8′-tetrahydro-ε,ψ-caroten-18-ol; Methyl3′,4′-didehydro-β,ψ-caroten-16′-oate; Methyl1-hexosyl-1,2-dihydro-3,4-didehydro-apo-8′-lycopenoate; Methyl hydrogen6,6′-diapocarotene-dioate; Methyl1-mannosyloxy-3,4-didehydro-1,2-dihydro-8′-apo-ψ-caroten-8′-oate; Methyl1′-methoxy-4′-oxo-1′,2′-dihydro-X,ψ-caroten-16 (or 17 or 18)-oate;2′-(O-Methyl-5-C-methylpentosyloxy)-3′,4′-didehydro-1′,2′-dihydro-β,ψ-carotene-3,1′-diol;Metridene; Mimulaxanthin; Monadoxanthin; Monoanhydrobacterioruberin;Monodehydro-β-carotene; Monodehydrolycopene; Monodemethyl(ated)spirilloxanthin; Monoepoxy-, see Epoxy-Monohydroxy cyclophytoene;Monohydroxy cyclophytofluene; Mutatochrome; Mutatoxanthin;Mytiloxanthin; Mytiloxanthinone; Myxobactin; Myxobactone; Myxol2′-glucoside; Myxol 2′-O-methyl-methylpentoside; Myxol 2′-rhamnoside;Myxoxanthin; Myxoxanthol; Myxoxanthophyll;

Neocarotene; Neochrome; Neo-β-carotene B; Neo-β-cryptoxanthin A;Neoxanthin; Neoxanthin 3-acetate; Neurosporaxanthin; Neurosporaxanthinmethyl ester; Neurosporene; Nonaprenoxanthin; 2′-Nor-astaxanthindiester; Norbixin; Nostoxanthin;

Octahydro-β-carotene; 1,2,7,8,11,12,7′,8′-Octahydro-ψ,ψ-carotene;7,8,11,12,7′,8′,11′,12′-Octahydro-ψ,ψ-carotene;1,2,7,8,11,12,7′,8′-Octahydro-ψ,ψ-carotene-1,2-diol;1,2,7,8,1′,2′,7,8′-Octahydro-ψ,ψ-carotene-1,1′-diol;1,2,7,8,11,12,7′,8′-Octahydro-ψ,ψ-caroten-1-ol;7,8,11,12,7,′,8′,11′,12′-Octahydro-β,ψ-caroten-2-ol;1,2,7,8,7′,8′,11′,12′-Octahydro-ψ,ψ-caroten-1-ol;7,8,11,12,7′,8′,11′,12′-Octahydro-4,4′-diapocarotene; Octahydrolycopene;5,6,7,8,5′,6′,7′,8′-Octydrolycopene;7,8,11,12,7′,8′,11′,12′-Octahydrolycopene;3,4,3′,4′,7′,8′,11′,12′-Octahydrospirilloxanthin; OH, see also Hydroxy-or -ol OH-Chlorobactene; OH-Chlorobactene glucoside; OH-Lycopene;2-OH-Monocyclophytoene; 2-OH-Monocyclophytofluene; OH-Neurosporene;OH-Okenone; OH—P 481; OH—P 482; OH—P 511; OH—R; OH-Rhodopin;OH-Sintaxanthin 5,6-epoxide; OH-Spheroidene; OH-Spheroidenone;OH-7,8,11,12-Tetrahydrolycopene; OH—Y; Okenone; Ophioxanthin;Oscillaxanthin; Oscillol 2,2′-di(O-methyl-methylpentoside); Oscillol2,2′-dirhamnoside; Ovoester; Oxo-, see also Keto or -one3-Oxocanthaxanthin; 4′-Oxo-4,4′-diapocaroten-4-oic acid;8′-Oxo-8,8′-diapocarotenoic acid; 3-Oxoechinenone; 4-Oxosaproxanthin;16′-Oxotorulene; 6′-Oxychrysanthemaxanthin;

P 412; P 444; P 450; P 452; P 481; P 500; P 518;1′-[(χ-O-Palmitoyl-β,D-glucosyl)oxy]-3′,4′-didehydro-1,′,2′-dihydro-β,ψ-caroten-2′-ol;Papilioerythrin; Papilioerythrinone; Paracentrone; Parasiloxanthin;Pectenol; Pectenolone; Pectenoxanthin; Pentaxanthin; Peridinin;Peridininol; Persicachrome; Persicaxanthin; Phillipsiaxanthin;Philosamiaxanthin; Phleixanthophyll; Phleixanthophyll palmitate;Phoeniconone; Phoenicopterone; Phoenicoxanthin; Physalien; Physoxanthin;Phytoene; C₃₀-Phytoene; Phytoene 1,2-(ep)oxide; Phytoenol; Phytofluene;Phytofluene epoxide; Phytofluenol; Pigment R; Pigment X; Pigment Y;Plectaniaxanthin; Poly-cis-γ-carotene; Poly-cis-ψ-carotene;Poly-cis-lycopene; Prasinoxanthin; Prelycopersene pyrophosphate;Prephytoene pyrophosphate; Pro-γ-carotene; Prolycopene; Proneurosporene;Protetrahydrolycopene; Pseudo-α-carotene; Pyrenoxanthin; Pyrrhoxanthin;Pyrrhoxanthinol;

7-cis: Renieracistene; Renierapurpurin; Renieratene; Reticulaxanthin;Retinylidenetiglic acid; Retrobisdehydro(-β-)carotene;Retrodehydro(-β-)carotene; Retrodehydro-γ-carotene;Retrodehydrozeaxanthin; Rhamnopyranosyloxy-, seeRhamnosyloxy-2′-O-Rhamnosyl-4-ketomyxol; 2′-O-Rhamnosylmyxol;3′-Rhamnosyloxy-β,β-caroten-3-ol;1-Rhamnosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-carotene;2′-Rhamnosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-carotene-3,1′-diol;2′-Rhamnosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-carotene-3,4,1′-triol;1′-Rhamnosyloxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-3-ol;Rhodoauranxanthin; Rhodopin; Rhodopin(-20-)al; Rhodopinal glucoside;Rhodopin glucoside; Rhodopinol; Rhodopurpurin; Rhodotorulene;Rhodovibrin; Rhodoviolascin; Rhodoxanthin; Roserythrin; Rubichrome;Rubixanthin; Rubixanthin 5,6-epoxide; Rubixanthone;

Salmon acid; Salmoxanthin; Saproxanthin; Sarcinaxanthin; Sarcinaxanthindiglucoside; Sarcinaxanthin monoglucoside; Sarcinene;5,6Seco-β,β-carotene-5,6-dione; 5,6-Seco-β,ε-carotene-5,6-dione;Semi-α-carotenone; Semi-β-carotenone; Sidnyaxanthin; Sintaxanthin;Siphonaxanthin; Siphonein;Sodium-3,19-dihydroxy-7,8-di-dehydro-β,χ-carotene-3′,6′-dione 3-sulfate;Sodium-3,19-dihydroxy-3′,6′-dioxo-7,8-didehydro-β,χ-caroten-17′-al3-sulfate;Sodium-3,19,3′-trihydroxy-7,8-didehydro-6′-oxo-β,χ-caroten-17′-oate3-sulfate;Sodium-3,19,17′-trihydroxy-7,8-didehydro-β,χ-carotene-3′,6′-dione3-sulfate; Sphaerobolin; Spheroidene; Spheroidenone; Spirilloxanthin;Sulcatoxanthin;

Tangeraxanthin; Taraxanthin; Taraxanthin dipalmitate; Taraxien;Tareoxanthin; Tedaniaxanthin; Tedanin; Ternstroemiaxanthin; Tethyatene;7,8,7′,8′-Tetradehydroastaxanthin; 3,4,3′;4′-Tetradehydro-β,β-carotene;3,4,3′,4′-Tetradehydro-ψ,ψ-carotene;7,8,7′,8′-Tetradehydro-β,β-carotene-3,3′-diol;3,4,3′,4′-Tetradehydro-β,β-carotene-2,2′-dione;3′,4′,7′,8β-Tetradehydro-β,β-caroten-3-ol;3,4,3′,4′-Tetradehidrolycopene;6,7,6′,7′-Tetradehydro-5,6,5′,6′-tetrahydro-β,β-carotene-3,3′-diol;6,7,6′,7′-Tetradehydro-5,6,5′,6′-tetrahydro-β,β-carotene-3,5,3′,5′-tetrol;7,8,7′,8′-Tetradehydrozeaxanthin;3,4,3′,4′-Tetradehydrobisanhydrobacterioruberin;5,6,5′,6′-Tetrahydrocanthaxanthin; 7,8,7′,8′-Tetrahydrocapsorubin;Tetrahydro-β-carotene; 7,8,7′,8′-Tetrahydro-β,β-carotene;7′,8′,11′,12′-Tetrahydro-β,ψ-carotene;7′,8′,11′,12′-Tetrahydro-γ-carotene;7′,8′,11′,12′-Tetrahydro-γ,ψ-carotene; 1,2,7,8-Tetrahydro-ψ,ψ-carotene;1,2,1′,2′-Tetrahydro-ψ,ψ-carotene; 7,8,11,12-Tetrahydro-ψ,ψ-carotene;7,8,7′,8′-Tetrahydro-ψ,ψ-carotene;5,6,5′,6′-Tetrahydro-β,β-carotene-4,4′-diol;7,8,7′,8′-Tetrahydro-β,β-carotene-3,3′-diol;7′,8′,9′,10′-Tetrahydro-β,ψ-carotene-3,17′-diol;1,2,1′,2′-Tetrahydro-ψ,ψ-carotene-1,1′-diol;5,6,5′,6′-Tetrahydro-β,β-carotene4,4′-dione;5,6,5,6′-Tetrahydro-β,β-carotene-3,5,6,3′,5′,6′-hexol;1,2,7,8-Tetrahydro-ψ,ψ-caroten-1-ol;1,2,7′,8′-Tetrahydro-ψ,ψ-caroten-1-ol;7,8,11,12-Tetrahydro-4,4′-diapocarotene;7,8,7′,8′-Tetrahydro-4,4′-diapocarotene; Tetrahydrolycopene;1,2,1′,2′-Tetrahydrolycopene; 5,6,5′,6′-Tetrahydrolycopene;7,8,11,12-Tetrahydrolycopene; 7,8,7′,8′-Tetrahydrolycopene;7′,8′,11′,12′-Tetrahydrolycopene;1,2,1′,2′-Tetrahydrolycopene-1,1′-diol;1,2,1′,2′-Tetrahydroneurosporene; 3,4,11′,12′-Tetrahydrospheroidene;3,4,7,8-Tetrahydrospirilloxanthin; 3,4,3′,4′-Tetrahydrospirilloxanthin;3,4,3′,4′-Tetrahydrospirilloxanthin-20-al;5,6,5′,6′-Tetrahydro-3,4,3′,4′-tetrol 4,4′-disulfate;2,3,2′,3′-Tetrahydroxy-β,β-carotene-4,4′-dione;2,3,2′,3′-Tetrahydroxy-β,β-caroten-4-one;3,19,3′,17′-Tetrahydroxy-β,χ-caroten-6′-one 3-sulfate;3,5,3′,5′-Tetrahydroxy-6′,7′-didehydro-5,8,5′,6′-tetrahydro-β,β-carotenne;3,3′,5,5′-Tetrahydroxy-6′-hydro-7-dehydro-β-carotene;3,4,3′,4′-Tetrahydroxypirardixanthin;3,4,3′,4′-Tetrahydroxy-5,6,5′,6′-tetrahydro-β,β-carotene;(3,4,3′,4′)-Tetraketo-β-carotene; 4,5,4′,5′-Tetraketo-β-carotene;Thiothece-425; Thiothece-460; Thiothece-474; Thiothece-478;Thiothece-484; Thiothece-OH-484; Tilefishxanthin I; Tilefishxanthin H;Tilefishxanthin III; Tilefishxanthin IV; Torularhodin;Torularhodinaldehyde; Torularhodin methyl ester; Torulenal; Torulene;Torulenecarboxylic acid; 2,3,2′-Trihydroxy-β,β-caroten-4-one;3,3′,4′-Trihydroxy-β,β-caroten-4-one;3,4,3′-Trihydroxy-β,χ-caroten-6′-one;3,3′,5′-Trihydroxy-6′,7′-dehydro-α-carotene;3,3′,8′-Trihydroxy-7,8-didehydro-β,χ-carotene4,6′-dione;3,3′,8′-Trihydroxy-7,8-didehydro-β,χ-caroten-6′-one;3,19,3′-Trihydroxy-7,8-didehydro-β,χ-caroten-6′-one 3-sulfate;3,1′,2′-Trihydroxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;3,5,19-Trihydroxy-6,7-didehydro-5,6,7′,8′-tetrahydro-7′-apo-β-caroten-8′-one3-acetate 19-hexanoate;3,5,6′-Trihydroxy-6,7-didehydro-5,6,7′,8′-tetrahydro-β,ε-carotene-3′,8′-dione;3,5,3′-Trihydroxy-5,6-dihydro-β-carotene;3,3′,5′-Trihydroxy-5′,6′-dihydro-β-carotene 5′,6′-epoxide;3,19,3′-Trihydroxy-7,8-dihydro-β,ε-caroten-8-one;3,19,3′-Trihydroxy-7,8-dihydro-β,ε-caroten-8-one 19-laurate;3,6,3′-Trihydroxy-7,8-dihydro-γ,ε-caroten-8-one;3,3,′,19-Trihydroxy-7,8-dihydro-8-oxo-α-carotene;3,3′,6′-Trihydroxy-5,8-epoxy-α-carotene;3,4,4′-Trihydroxypirardixanthin;1,1′,2′-Trihydroxy-3,4,3′,4′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-caroten-2-one;3,4,4′-Trihydroxy-5,6,5′,6′-tetrahydro-β,β-carotene; Trikentriorhodin;3,4,4′-Triketo-β-carotene;3,1′,2′-Trimethoxy-3′,4′-didehydro-1′,2′-dihydro-β,ψ-caroten-4-one;Triophaxanthin; Triphasiaxanthin; Trisanhydrobacterioruberin; Trollein;Trollichrome; Trolliflavin; Trolliflor; Trollixanthin; Tunaxanthin;

Unidentified II; Unknown 370; Unknown 437; Uriolide;

Vaucheriaxanthin; Violaxanthin; Violeoxanthin; Violerythrin;

Warmingol; Warmingone; Webbiaxanthin;

Xanthophyll; Xanthophyll K₁; Xanthophyll K₁S; Xanthophyll dipalmitate;Xanthophyll epoxide;

α-Zeacarotene; β-Zeacarotene; β₁-Zeacarotene; α-Zeacarotene-3,17′-diol;β-Zeacarotene-3,17′-diol; β-Zeacaroten-3-ol; Zeaxanthene; Zeaxanthin;Zeaxanthin diepoxide; Zeaxanthin dimethyl ether; Zeaxanthindirhamnoside; Zeaxanthin dipalmitate; Zeaxanthin 5,6-epoxide; Zeaxanthin5,8-epoxide; Zeaxanthin furanoxide; Zeaxanthin monomethyl ether;Zeaxanthin monorhamnoside; Zeaxanthol; and Zeinoxanthin. The above listof naturally occurring carotenoids is meant to a be a non-limitingexample of naturally occurring carotenoids. The list is notcomprehensive as there are still more naturally occurring moleculeswhich have been discovered and to be discovered which will fall withinthe category of carotenoids.

In some embodiments, the total synthesis of naturally occurring as wellas synthetic carotenoids as starting scaffolds for carotenoid analogs orderivatives may be a method of generation of said carotenoid analogs orderivatives.

In some embodiments, the carotenoid derivatives may include compoundshaving a structure including a polyene chain (i.e., backbone of themolecule). The polyene chain may include between about 5 and about 15unsaturated bonds. In certain embodiments, the polyene chain may includebetween about 7 and about 12 unsaturated bonds. In some embodiments acarotenoid derivative may include 7 or more conjugated double bonds toachieve acceptable antioxidant properties.

In some embodiments, decreased antioxidant properties associated withshorter polyene chains may be overcome by increasing the dosageadministered to a subject or patient.

In some embodiments, a chemical compound including a carotenoidderivative may have the general structure (I):

Each R³ may be independently hydrogen or methyl. R¹ and R² may beindependently H, an acyclic alkene with one or more substituents, or acyclic ring including one or more substituents. y may be 5 to 12. Insome embodiments, y may be about 3 to about 15. In certain embodiments,the maximum value of y may only be limited by the ultimate size of thechemical compound, particularly as it relates to the size of thechemical compound and the potential interference with the chemicalcompound's biological availability as discussed herein. In someembodiments, substituents may be at least partially hydrophilic. Thesecarotenoid derivatives may be used in a pharmaceutical composition.

In some embodiments, the carotenoid derivatives may include compoundshaving the structure (Ia):

Each R³ may be independently hydrogen, methyl, alkyl, alkenyl, oraromatic substituents. R¹ and R² may be independently H, an acyclicalkene with at least one substituent, or a cyclic ring with at least onesubstituent having general structure (II):

where n may be between 4 to 10 carbon atoms. W is the substituent.

In some embodiments, each cyclic ring may be independently two or morerings fused together to form a fused ring system (e.g., a bycyclicsystem). Each ring of the fused ring system may independently containone or more degrees of unsaturation. Each ring of the fused ring systemmay be independently aromatic. Two or more of the rings forming thefused ring system may form an aromatic system.

In some embodiments, a chemical compound including a carotenoidderivative may have the general structure (Ib):

Each R³ may be independently hydrogen or methyl. Each Y may beindependently O or H₂. Each R may be independently OR¹ or R¹. Each R¹may be independently -alkyl-NR² ₃ ⁺, aromatic-NR² ₃ ⁺, -akyl-CO₂ ⁻,-aromatic-CO₂ ³¹ , -amino acid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, H, alkyl, or aryl. Each R² may beindependently H, alkyl, or aryl. z may be 5 to 12. In some embodiments,z may be about 3 to about 15. In certain embodiments, the maximum valueof z may only be limited by the ultimate size of the chemical compound,particularly as it relates to the size of the chemical compound and thepotential interference with the chemical compound's biologicalavailability as discussed herein. In some embodiments, substituents maybe at least partially hydrophilic. These carotenoid derivatives may beused in a pharmaceutical composition.

In some embodiments, a chemical compound including a carotenoidderivative may have the general structure (Ic):

Each R³ may be independently hydrogen or methyl. Each Y may beindependently O or H₂. Each X is independently

-alkyl-NR¹ ₃ ⁺, -aromatic-NR¹ ₃ ⁺, -alkyl-CO₂ ⁻, -aromatic-CO₂ ⁻, -aminoacid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺, polyethylene glycol,dextran, alkyl, or aryl. Each R¹ is independently -alkyl-NR² ₃ ⁺,-aromatic-NR² ₃ ⁺, -alkyl-CO₂ ⁻, -aromatic-CO₂ ⁻, -amino acid-NH₃ ⁺,-phosphorylated amino acid-NH₃ ⁺, polyethylene glycol, dextran, H,alkyl, aryl, or alkali salt. Each R² may be independently H, alkyl, oraryl. z may be 5 to 12. In some embodiments, z may be about 3 to about15. In certain embodiments, the maximum value of z may only be limitedby the ultimate size of the chemical compound, particularly as itrelates to the size of the chemical compound and the potentialinterference with the chemical compound's biological availability asdiscussed herein. In some embodiments, substituents may be at leastpartially hydrophilic. These carotenoid derivatives may be used in apharmaceutical composition.

In some non-limiting examples, five- and/or six-membered ring carotenoidderivatives may be more easily synthesized. Synthesis may come moreeasily due to, for example, the natural stability of five- andsix-membered rings. Synthesis of carotenoid derivatives including five-and/or six-membered rings may be more easily synthesized due to, forexample, the availability of naturally occurring carotenoids includingfive- and/or six-membered rings. In some embodiments, five-memberedrings may decrease steric hindrance associated with rotation of thecyclic around the molecular bond connecting the cyclic ring to thepolyene chain. Reducing steric hindrance may allow greater overlap ofany π oribitals within a cyclic with the polyene chain, therebyincreasing the degree of conjugation and effective chromophore length ofthe molecule. This may have the salutatory effect of increasingantioxidant capacity of the carotenoid derivatives.

In some embodiments, a substituent (W) may be at least partiallyhydrophilic. A hydrophilic substituent may assist in increasing thewater solubility of a carotenoid derivative. In some embodiments, acarotenoid derivative may be at least partially water soluble. Thecyclic ring may include at least one chiral center. The acyclic alkenemay include at least one chiral center. The cyclic ring may include atleast one degree of unsaturation. In some cyclic ring embodiments, thecyclic ring may be aromatic. One or more degrees of unsaturation withinthe ring may assist in extending the conjugation of the carotenoidderivative. Extending conjugation within the carotenoid derivative mayhave the salutatory effect of increasing the antioxidant properties ofthe carotenoid derivatives. The cyclic ring may include a substituent.The substituent may be hydrophilic. In some embodiments, the cyclic ringmay include, for example (a), (b), or (c):

In some embodiments, the substituent may include, for example, acarboxylic acid, an amino acid, an ester, an alkanol, an amine, aphosphate, a succinate, a glycinate, an ether, a glucoside, a sugar, ora carboxylate salt.

In some embodiments, each substituent —W may independently include —XR.Each X may independently include O, N, or S. In some embodiments, eachsubstituent —W may independently comprises amino acids, esters,carbamates, amides, carbonates, alcohol, phosphates, or sulfonates. Insome substituent embodiments, the substituent may include, for example(d) through (rr):

where each R is, for example, independently -alkyl-NR¹ ₃ ⁺,-aromatic-NR¹ ₃ ⁺, -alkyl-CO₂ ³¹ , -aromatic-CO₂ ³¹ , -amino acid-NH₃ ³⁰, -phosphorylated amino acid-NH₃ ³⁰ , polyethylene glycol, dextran, H,alkyl, or aryl. In some embodiments, substituents may include anycombination of (d) through (rr). In some embodiments, negatively chargedsubstituents may include alkali metals, one metal or a combination ofdifferent alkali metals in an embodiment with more than one negativelycharged substituent, as counter ions. Alkali metals may include, but arenot limited to, sodium, potassium, and/or lithium.

Water soluble carotenoid analogs or derivatives may have a watersolubility of greater than about 1 mg/mL in some embodiments. In certainembodiments, water soluble carotenoid analogs or derivatives may have awater solubility of greater than about 10 mg/mL. In some embodiments,water soluble carotenoid analogs or derivatives may have a watersolubility of greater than about 50 mg/mL.

The absolute size of a carotenoid derivative (in 3 dimensions) isimportant when considering its use in biological and/or medicinalapplications. Some of the largest naturally occurring carotenoids are nogreater than about C₅₀. This is probably due to size limits imposed onmolecules requiring incorporation into and/or interaction with cellularmembranes. Cellular membranes may be particularly co-evolved withmolecules of a length of approximately 30 nm. In some embodiments,carotenoid derivatives may be greater than or less than about 30 nm insize. In certain embodiments, carotenoid derivatives may be able tochange conformation and/or otherwise assume an appropriate shape whicheffectively enables the carotenoid derivative to efficiently interactwith a cellular membrane.

Although the above structure, and subsequent structures, depict alkenesin the E configuration this should not be seen as limiting. Compoundsdiscussed herein may include embodiments where alkenes are in the Zconfiguration or include alkenes in a combination of Z and Econfigurations within the same molecule. The compounds depicted hereinmay naturally convert between the Z and E configuration and/or exist inequilibrium between the two configurations.

In an embodiment, a chemical compound may include a carotenoidderivative having the structure (III)

Each Y may be independently O or H₂. Each R may be independently OR¹ orR¹. Each R¹ may be independently -alkyl-NR² ₃ ³⁰ , -aromatic-NR² ₃ ³⁰ ,-alkyl-CO₂ ⁻, -aromatic-CO₂ ⁻, -amino acid-NH₃ ⁺, -phosphorylated aminoacid-NH₃ ⁺, polyethylene glycol, dextran, H, alkyl, peptides,poly-lysine or aryl. In addition, each R² may be independently H, alkyl,or aryl. The carotenoid derivative may include at least one chiralcenter.

In a specific embodiment where Y is H₂, the carotenoid derivative hasthe structure (IV)

In a specific embodiment where Y is O, the carotenoid derivative has thestructure (V)

In an embodiment, a chemical compound may include a carotenoidderivative having the structure (VI)

Each Y may be independently O or H₂. Each R may be independently H,alkyl, or aryl. The carotenoid derivative may include at least onechiral center. In a specific embodiment Y may be H₂, the carotenoidderivative having the structure (VII)

In a specific embodiment where Y is O, the carotenoid derivative has thestructure (VIII)

In an embodiment, a chemical compound may include a carotenoidderivative having the structure (IX)

Each Y may be independently O or H₂. Each R′ may be CH₂. n may be 1 to9. Each X may be independently

Each R may be independently -alkyl-NR¹ ₃ ⁺, -aromatic-NR¹ ₃ ⁺,-alkyl-CO₂ ⁻, -aromatic-CO₂ ⁻, -amino acid-NH₃ ⁺, -phosphorylated aminoacid-NH₃ ⁺, polyethylene glycol, dextran, H, alkyl, or aryl. Each R¹ maybe independently H, alkyl, or aryl. The carotenoid derivative mayinclude at least one chiral center.

In a specific embodiment where Y is H₂, the carotenoid derivative hasthe structure (X)

In a specific embodiment where Y is O, the carotenoid derivative has thestructure (XI)

In an embodiment, a chemical compound may include a carotenoidderivative having the structure (XII)

Each Y may be independently O or H₂. The carotenoid derivative mayinclude at least one chiral center. In a specific embodiment Y may beH₂, the carotenoid derivative having the structure (XIII)

In a specific embodiments where Y may O, the carotenoid derivative hasthe structure (XIV)

In some embodiments, a chemical compound may include a disuccinic acidester carotenoid derivative having the structure (XV)

In some embodiments, a chemical compound may include a disodium saltdisuccinic acid ester carotenoid derivative having the structure (XVI)

In some embodiments, a chemical compound may include a carotenoidderivative with a co-antioxidant, in particular one or more analogs orderivatives of vitamin C (i.e., L ascorbic acid) coupled to acarotenoid. Some embodiments may include carboxylic acid and/orcarboxylate derivatives of vitamin C coupled to a carotenoid (e.g.,structure (XVII))

Carbohydr. Res. 1978, 60, 251-258, herein incorporated by reference,discloses oxidation at C-6 of ascorbic acid as depicted in EQN. 5.

Some embodiments may include vitamin C and/or vitamin C analogs orderivatives coupled to a carotenoid. Vitamin C may be coupled to thecarotenoid via an ether linkage (e.g., structure (XVIII))

Some embodiments may include vitamin C disuccinate analogs orderivatives coupled to a carotenoid (e.g., structure (XIX))

Some embodiments may include solutions or pharmaceutical preparations ofcarotenoids and/or carotenoid derivatives combined with co-antioxidants,in particular vitamin C and/or vitamin C analogs or derivatives.Pharmaceutical preparations may include about a 2:1 ratio of vitamin Cto carotenoid respectively.

In some embodiments, co-antioxidants (e.g., vitamin C) may increasesolubility of the chemical compound. In certain embodiments,co-antioxidants (e.g., vitamin C) may decrease toxicity associated withat least some carotenoid analogs or derivatives. In certain embodiments,co-antioxidants (e.g., vitamin C) may increase the potency of thechemical compound synergistically. Co-antioxidants may be coupled to acarotenoid derivative. Co-antioxidants may coupled (e.g., a covalentbond) to the carotenoid derivative. Co-antioxidants may be included as apart of a pharmaceutically acceptable formulation.

In some embodiments, a carotenoid (e.g., astaxanthin) may be coupled tovitamin C forming an ether linkage. The ether linkage may be formedusing the Mitsunobu reaction as in EQN. 1.

In some embodiments, vitamin C may be selectively esterified. Vitamin Cmay be selectively esterified at the C-3 position (e.g., EQN. 2). J.Org. Chem. 2000, 65, 911-913, herein incorporated by reference,discloses selective esterification at C-3 of unprotected ascorbic acidwith primary alcohols.

In some embodiments, a carotenoid may be coupled to vitamin C. Vitamin Cmay be coupled to the carotenoid at the C-6, C-5 diol position asdepicted in EQNS. 3 and 4 forming an acetal.

In some embodiments, a carotenoid may be coupled to a water solublemoiety (e.g., vitamin C) with a glyoxylate linker as depicted in EQN. 6.Tetrahedron 1989, 22, 6987-6998, herein incorporated by reference,discloses similar acetal formations.

In some embodiments, a carotenoid may be coupled to a water solublemoiety (e.g., vitamin C) with a glyoxylate linker as depicted in EQN. 7.J. Med. Chem. 1988, 31, 1363-1368, herein incorporated by reference,discloses the glyoxylic acid chloride.

In some embodiments, a carotenoid may be coupled to a water solublemoiety (e.g., vitamin C) with a phosphate linker as depicted in EQN. 8.Carbohydr. Res. 1988, 176, 73-78, herein incorporated by reference,discloses the L-ascorbate 6-phosphate.

In some embodiments, a carotenoid may be coupled to a water solublemoiety (e.g., vitamin C) with a phosphate linker as depicted in EQN. 9.Carbohydr. Res. 1979, 68, 313-319, herein incorporated by reference,discloses the 6-bromo derivative of vitamin C. Carbohydr. Res. 1988,176, 73-78, herein incorporated by reference, discloses the 6-bromoderivative of vitamin C's reaction with phosphates.

In some embodiments, a carotenoid may be coupled to a water solublemoiety (e.g., vitamin C) with a phosphate linker as depicted in EQN. 10.J. Med Chem. 2001, 44, 1749-1757 and J. Med Chem. 2001, 44, 3710-3720,herein incorporated by reference, disclose the allyl chloride derivativeand its reaction with nucleophiles, including phosphates, under mildbasic conditions.

In some embodiments, a carotenoid may be coupled to a water solublemoiety (e.g., vitamin C) with a phosphate linker as depicted in EQN. 11.Vitamin C may be coupled to the carotenoid using selectiveesterification at C-3 of unprotected ascorbic acid with primaryalcohols.

In some embodiments, a carotenoid may be coupled to a water solublemoiety (e.g., vitamin C) with a phosphate linker as in LXVII. StructureLXVII may include one or more counterions (e.g., alkali metals).

EQN. 12 depicts an example of a synthesis of a protected form of LXVII.

In some embodiments, a chemical compound may include a carotenoidderivate including one or more amino acids (e.g., lysine) and/or aminoacid abalogs or derivatives (e.g., lysine hydrochoric acid salt) coupledto a carotenoid [e.g., structure(XX)].

In some embodiments, a carotenoid analog or derivative may include:

In an embodiment, the carotenoid derivatives may be synthesized fromnaturally occuring carotenoids. The carotenids may include structures2A-2E depicted in FIG. 1. In some embodiments, the carotenoidderivatives may be synthesized from a naturally occurring carotenoidincluding one or more alcohol substituents. In other embodiments, thecarotenoid derivatives may be synthesized from a derivative of anaturally occurring carotenoid including one or more alcoholsubstituents. The synthesis may result in a single stereoisomer. Thesynthesis may result in a single geometric isomer of the carotenoidderivative. The synthesis/synthetic sequence may include any priorpurification or isolation steps carried out on the parent carotenoid.The synthesis may be a total synthesis. An example may include, but isnot limited to, a 3S,3′S all-E carotenoid derivative, where the parentcarotenoid is astaxanthin. The synthetic sequence may include protectingand subsequently deprotecting various functionalities of the carotenoidand/or substituent precursor. The alcohols may be deprotonated with abase. The deprotonated alcohol may be reacted with a substituentprecursor with a good leaving group. The base may include anynon-nucleophilic base known to one skilled in the art such as, forexample, dimethylaminopyridine (DMAP). The deprotonated alcohol may actas a nucleophile reacting with the substituent precursor, displacing theleaving group. Leaving goups may include, but are not limited to, Cl,Br, tosyl, brosyl, mesyl, or trifyl. These are only a few examples ofleaving groups that may be used, many more are known and would beapparent to one skilled in the art. In some embodiments, it may not evenbe necessary to deprotonate the alcohol, depending on the leaving groupemployed. In other examples the leaving group may be internal and maysubsequently be included in the final structure of the carotenoidderivative, a non-limiting example may include anhydrides or strainedcyclic ethers. For example, the deprotonated alcohol may be reacted withsuccinic anhydride. In an embodiment, the disuccinic acid ester ofastaxanthin may be further converted to the disodium salt. Examples ofsynthetic sequences for the preparation of some of the specificembodiments depicted are described in the Examples section. The exampledepicted below is a generic non-limiting example of a synthetic sequencefor the preparation of carotenoid derivatives.

Ischemia-Reperfusion (I/R) Injury: Pathophysiologic Features

Reperfusion of ischemic myocardium results in significant cellular andlocal alterations in at-risk tissue which exacerbate damage created bythe ischemic insult. Specifically, vascular and microvascular injury,endothelial dysfunction, accelerated cellular necrosis, and granulocyteactivation occur subsequent to ischemia-reperfusion. Vascular andmicrovascular injury results from complement activation, the interactionof circulating and localized C-reactive protein with C1q andphosphocholine on exposed cells forming the membrane attack complex(MAC) with ensuing cell death and increased endothelial permeability,superoxide anion (O₂—) generation by affected endothelium and activatedleukocytes, microemboli, cytokine release (in particular IL-6), andactivation of platelets with IIbIIIa receptor activation, and subsequentrelease of ADP and serotonin. Endothelial dysfunction follows, withsubsequent generation of superoxide anion by the dysfunctionalendothelium, further damaging the affected endothelium in a positivefeedback cycle. It has been shown that ischemia-reperfusion results inearly and severe injury to the vasculature, which further compromisesmyocyte survival. Granulocyte activation also occurs duringischemia-reperfusion. The activation and degranulation of this celllineage results in the release of myeloperoxidase (MPO), elastases,proteases, and oxygen-derived radical and non-radical species (mostimportantly superoxide anion, hypochlorite, singlet oxygen, and hydrogenperoxide after the “respiratory burst”). Oxygen-derived radical andnon-radical (e.g. singlet oxygen) species are implicated in much of thedamage associated with ischemia and reperfusion, and lipid peroxidationhas clearly been shown to be a sequel of reperfusion as measured bythiobarbituric acid reactive substances (TBARS), malondialdehyde (MDA),or conjugated diene formaton.

The ischemic insult to both the endothelium of coronary vessels and themyocardium itself creates conditions favoring the production of radicalsand other non-radical oxygen-derived species capable of damaging tissueherein collectively referred to as reactive oxygen species (“ROS”). Theendothelium-based xanthine dehydrogenase-xanthine oxidase system inhumans is a source of the superoxide anion (O₂—). The human myocardiumlacks this enzyme system. In healthy tissue, 90% of the enzyme exists asthe dehydrogenase (D) form; it is converted to the oxidase (O) form inischemic tissue. The (O)-form, using molecular oxygen as the electronacceptor, produces the superoxide anion O₂— in the coronary endothelium.Superoxide anion is then available to create additional tissue damage inthe local environment. The superoxide anion is not the most reactive ordestructive radical species in biological systems on its own. However,it is the source of some shorter- and longer-lived, more damagingradicals and/or ROS such as the hydroxyl radical, hydrogen peroxide,singlet oxygen, and peroxyl radicals (e.g. peroxynitrite). As such, itcan be considered the “lynchpin” radical in I/R injury. The biologicalreactions of the superoxide radical to form these important oxidants areshown below:

-   (1) superoxide anion may accept a single electron (“monovalent    reduction”), producing peroxide (O₂ ⁻²). Coupled with 2 protons,    peroxide then forms hydrogen peroxide (H₂O₂). H₂O₂ diffuses easily    through cell membranes and cannot readily be excluded from the    cytoplasm, where it may react with cellular components or activate    central inflammatory cascades such as nuclear factor kappa-B    (NF-kappa-B), which are also implicated in the additional    inflammatory damage in I/R injury.-   (2) superoxide anion typically reacts with itself to produce    hydrogen peroxide and oxygen (“dismutation”). Superoxide dismutation    may be spontaneous, or catalyzed by the enzyme superoxide dismutase    (SOD), a reaction which results in the formation of oxidized SOD:    2O₂ ⁻+2H⁺→H₂O₂+³O₂-   (3) superoxide anion may serve as a reducing agent and donate a    single electron (“monovalent reduction”) to a metal cation. For    example, in the two step process below, ferric iron (Fe³⁺) is    reduced and subsequently acts as a catalyst to convert hydrogen    peroxide (H₂O₂) into the hydroxyl radical (HO.).    O₂ ⁻+Fe³⁺→³O₂+Fe²⁺   (step 1)-   Ferrous iron (Fe²⁺), the reduced metal cation, subsequently    catalyzes the breaking of the oxygen-oxygen bond of hydrogen    peroxide. This produces one hydroxyl radical (HO.) and one hydroxide    ion (HO⁻). The reaction is known as the Fenton reaction,    particularly important in ischemia-reperfusion injury where iron    and/or copper compartmentalization has been lost (typically through    hemolysis of red blood cells, RBCs):    Fe²⁺+H₂O₂→Fe³⁺+HO.+HO⁻   (step 2)-   Hydroxyl radicals readily cross cellular membranes. Hydroxyl radical    damage is “diffusion rate-limited”, that is, the 3-dimensional    distance in which damage may be inflicted is related to the    radical's rate of diffusion. The hydroxyl radical is a particularly    toxic ROS. Hydroxyl radicals may add to organic substrates    (represented by R in the reaction below) and form a hydroxylated    adduct which is itself a radical. In the case of    ischemia-reperfusion injury, polyunsaturated fatty acids (PUFAs) in    endothelial and myocyte membranes are particularly susceptible to    hydroxyl radical damage:    HO.+R→HOR.  (hydroxylated adduct)-   The adduct formed above may further oxidize in the presence of metal    cations or molecular oxygen. This results in oxidized, stable    product(s). In the first case, the extra electron is transferred to    the metal ion, and in the second case, to oxygen (forming    superoxide). Two adduct radicals may also react with each other    forming oxidized, stable, and crosslinked products plus water. This    is an important process in the oxidation of membrane proteins:    HOR.+HOR.→R—R+2H₂O-   In addition, hydroxyl radicals may oxidize organic substrates by    abstracting electrons from such molecules:    HO.+R→R.+OH⁻-   The oxidized substrate (R.) is a radical. Such radicals may react    with other molecules in a chain reaction. Carotenoids are    particularly efficient lipid-peroxidation chain breakers. In one    instance, the reaction with ground-state oxygen produces peroxyl    radicals (ROO.):    R.+³O₂→ROO.-   Peroxyl radicals are very reactive. They may react with other    organic substrates in a chain reaction:    ROO.+RH→ROOH+R.-   Chain reactions are common in the oxidative damage of PUFAs and    other susceptible membrane lipids. Measurement of the rate of oxygen    consumption is one indication of the initiation and progress of the    chain reaction. It is important to note that, in liposomal model    systems, non-esterified, free astaxanthin at the appropriate dose is    capable of complete suppression of the chain reaction and    accompanying oxygen consumption.-   (4) superoxide anion may react with the hydroxyl radical (HO.) to    form singlet oxygen (¹O₂*). Singlet oxygen is not a radical, but is    highly reactive and damaging in cardiac biological systems. Singlet    oxygen has been implicated in the destruction of membrane-bound    proteins such as 5′-nucleotidase, important in the maintenance or    restoration of local concentrations of vasodilatory compounds such    as adenosine (shown to be effective in humans for reduction of    infarct size):    O₂ ⁻+HO.→¹O₂*+HO⁻-   (5) superoxide anion may also react with the radical nitric oxide    (NO⁻), producing peroxynitrite (OONO⁻). Peroxynitrite is a highly    reactive and damaging molecule in biological systems.    O₂ ⁻+NO.→OONO⁻

Polymorphonuclear leukocytes (PMNs), in particular neutrophils, andactivated macrophages are a rich source of oxygen-derived radical andnon-radical species. The NADPH-oxidase system located in phagocyte cellmembranes is an important source of radicals following stimulation. ThePMNs and activated macrophages rapidly consume oxygen in the“respiratory burst” and convert it to superoxide anion and subsequentlyhydrogen peroxide (H₂O₂), as well as significant amounts of singletoxygen. PMNs are additionally a source of hypochlorite, another damagingreactive oxygen species. While important in phagocytic cell activity ininfection, in the local environment during ischemia and reperfusion,further cellular injury occurs as these ROS attack normal and damagedhost cells in the local area.

Neutrophils are a primary source of oxygen radicals duringischemia-reperfusion after prolonged myocardial ischemia, particularlyin animal models of experimental infarction. Many prior studies havedocumented oxygen radical formation during ischemia-reperfusion, but fewaddressed the source(s) of such radicals in vivo, or had examinedradical generation in the context of prolonged myocardial ischemia.Neutrophils are recruited in large amounts within the previouslyischemic tissue and are thought to induce injury by local release ofvarious mediators, chiefly oxygen radicals. Previously, the contributionof activated neutrophils to ischemia-reperfusion injury and potentialmyocardial salvage remained unclear. A methodology was developed todetect radicals, in particular superoxide anion, without interferingwith the blood-borne mechanisms of radical generation.

Open- and closed-chest dogs underwent aorta and coronary sinuscatheterization (Duilio et al. 2001). No chemicals were infused.Instead, blood was drawn into syringes pre-filled with a spin trap andanalyzed by electron paramagnetic resonance (EPR) spectroscopy. After 90minutes of coronary artery occlusion, the transcardiac concentration ofoxygen radicals rose several-fold 10 minutes after reflow, and remainedsignificantly elevated for at least 1 hour. Radicals were mostly derivedfrom neutrophils, in particular superoxide anion. These radicalsexhibited marked reduction after the administration of (1) neutrophilNADPH-oxidase inhibitors and (2) a monoclonal antibody (R15.7) againstneutrophil CD18-adhesion molecule. The first intervention was designedto reduce the neutrophil respiratory burst, and the second to reducerecruitment of neutrophils to the site(s) of ischemia-reperfusioninjury. The reduction of radical generation by the monoclonal antibodyR15.7 was also associated with a significantly smaller infarct size andwith a concomitant decrease in no-reflow areas. It was demonstrated forthe first time that activated neutrophils were a major source ofoxidants in hearts reperfused in vivo after prolonged ischemia, thatthis phenomenon was long-lived, and that anti-neutrophil interventionscould effectively prevent the increase in transcardiac concentration ofoxygen radicals during reperfusion. In these animal models ofexperimental infarction, the lack of pre-existing pathology prior tocoronary artery occlusion may over-emphasize the contribution ofneutrophilic recruitment and activation to I/R injury; indeed, in thehuman atherosclerotic situation, activated macrophages and activatedT-lymphocytes already residing in the “area-at-risk” may also contributesubstantially to I/R injury. These resident inflammatory cellsthemselves are also sources of superoxide anion and other ROS.

Ischemia causes depletion of ATP in cells in the affected area. At thelevel of the mitochondrial electron transport chain, which normally“leaks” approximately 5% of the processed electrons in healthy tissue,further leakage of partially-reduced oxygen species (in particular O₂—)is favored when the respiratory chain becomes largely reduced. Thishappens primarily during ischemia. The net effect in the local cellularenvironment is a tip in the balance of the redox status fromanti-oxidant to pro-oxidant, which is at the same time less capable ofabsorbing additional radical insult(s) without further cellular damage.

Prevention of Ischemia-Reperfusion Injury: Pharmacologic Agents Used inPrevious Animal and/or Human Trials

The following compounds have been evaluated, either in animal models orin limited human trials, as therapeutic agents for the reduction ofischemia-reperfusion injury and/or myocardial salvage during acutemyocardial infarction (AMI). Most are biological antioxidants.

-   -   Superoxide dismutase (and derivatives or mimetics)    -   Catalase    -   Glutathione and glutathione peroxidase    -   Xanthine oxidase inhibitors    -   Vitamins B, C, E (and derivatives)    -   Calcium antagonists    -   ACE inhibitors    -   Sulphydryl thiol compounds (in particular N-acetylcysteine)    -   Iron chelators (desferioxamine)    -   Anti-inflammatories (e.g., ibuprofen)    -   Phosphocreatine    -   N-2-mercaptopropionyl glycine (MPG)    -   Probucol (and derivatives)    -   Melatonin    -   Coenzyme Q-10

Seminal work by Singh and co-workers in India previously demonstratedthat human patients presenting with acute myocardial infarction aredepleted in endogenous antioxidants, and that supplementation withantioxidant cocktails and/or monotherapy with coenzyme Q10 (a potentlipophilic antioxidant) were useful to achieve both myocardial salvageand improvement in traditional hard clinical endpoints (such as totalcardiac deaths and nonfatal reinfarction) at 30 days post-AMI. TheAMISTAD trials demonstrated the usefulness of adenosine as a myocardialsalvage agent in 3 separate groups of patients. RheothRx™ (a Theologicalagent) was also efficacious as a salvage agent in human trials, but wasabandoned secondary to renal toxicity. Most recently, Medicure, Inc.demonstrated the utility of a vitamin B derivative for myocardialsalvage in a small Phase II pilot study in collaboration with the DukeClinical Research Institute. Hence, the “translational” problem (fromefficacy in animal models of experimental infarction to human clinicalefficacy) identified in previous reviews of I/R injury is now betterunderstood. However, the commercial window-of-opportunity still exists,as no agent has been specifically approved for human use as a salvageagent.

Timing of Treatment For Myocardial Ischemia-Reperfusion Injury

As discussed above, early reperfusion of acute myocardial infarctions(primarily with pharmacological or surgical reperfusion) halts celldeath due to ischemia, but paradoxically causes further injury—mostlikely by oxidant mechanisms. Horwitz et al. (1999) identified thewindow of opportunity during which antioxidants must be present intherapeutic concentrations to prevent reperfusion injury during 90minutes of ischemia, and 48 hours of subsequent reperfusion, in 57 dogs.Statistical analyses in the trial focused on identifying components ofgroup membership responsible for differences in infarct size, andrevealed that duration of treatment was a major determinant. If begun atany time within the first hour of reperfusion, infusions of greater thanor equal to 3 hours markedly diminished infarct size. Duilio et al.(2001) further clarified this issue by demonstrating that oxygenconsumption reflective of the peroxyl radical chain reaction begins 10minutes after reperfusion, and that radical activity remains elevatedfor at least the first hour of reperfusion in a canine model. Singh etal. (1996) previously demonstrated in human patients that myocardialsalvage, and improvement of hard clinical endpoints (nonfatalreinfarction, death) was possible starting antioxidant therapy onaverage 13 hours post-MI, and continuing for 28 days. Therefore, plasmaantioxidants with long half-lives may be particularly appropriate forthis setting, as they may be administered as a loading dose and allowedto decay in the plasma throughout the critical early post-AMI period (0to 24 hours). The plasma half-lives of carotenoids administered orallyrange from approximately 21 hours for the xanthophylls (“oxygenated”carotenoids including astaxanthin, capsanthin, lutein, and zeaxanthin)to 222 hours for carotenes (“hydrocarbon” carotenoids such as lycopene).The significant difference in plasma antioxidant half-life (7 minutes)in the trial by Horwitz et al. (1999), for superoxide dismutase and itsmimetics in human studies, versus a nearly 21 hour half-life forxanthophylls and nearly 9 days for carotenes, highlights thepharmacokinetic advantages and potential cardioprotection against I/Rinjury by carotenoids in AMI in humans.

Critical Appraisal of Antioxidants in Ischemia-Reperfusion Injury: HumanStudies

Mean levels of vitamins A, C, E, and β-carotene were significantlyreduced in patients presenting with AMI, compared with control patientsin a study conducted by Singh et al. (1994). Lipid peroxides weresignificantly elevated in the AMI patients. The inverse relationshipbetween AMI and low plasma levels of vitamins remained significant afteradjustment for smoking and diabetes in these patients. Similarly, 38patients with AMI were studied by Levy et al. (1998), and exhibitedsignificantly decreased levels of vitamins A, E, and β-carotene comparedwith age-matched, healthy control subjects. After thrombolysis, lipidperoxidation products increased significantly in the serum of treatedpatients. Thrombolytic therapy also caused a significant decrease inplasma vitamin E levels. These descriptive studies indicate that uponpresentation with AMI, it is likely that serum levels of antioxidantvitamins will be decreased in patients undergoing an acute coronaryevent. Pharmacologic intervention with antioxidant compounds in theacute setting would likely remedy deficiencies in antioxidant vitaminsand total body antioxidant status.

Prospective human intervention trials with antioxidants in the settingof primary and/or secondary prevention of CVD are similarly limited, buthave been largely successful. Four out of five recent human studiesstrongly support the effectiveness of vitamin E in reducing heartdisease risk and complication rates. The Secondary Prevention withAntioxidants of Cardiovascular Disease in End-Stage Renal Disease study,in patients with significant kidney disease, revealed a 70% reduction innonfatal MI in patients given 800 IU per day of natural source vitaminE. Similarly, as mentioned herein, a number of agents have now beensuccessfully applied to myocardial salvage applications in humans.

Delivery of a low molecular weight compound intravenously in the acutesetting to inhibit or ameliorate I/R injury will require an evaluationof its immunogenicity. The incidence of transfusion-type and otheradverse reactions to the rapid infusion of the compound must beminimized. Compounds with a molecular weight <1000 Da, e.g. aspirin,progesterone, and astaxanthin, are likely not immunogenic unlesscomplexed with a carrier. As molecular weight increases to between 1000and 6000 Da, e.g. insulin and ACTH, the compound may or may not beimmunogenic. As molecular weight increases to >6000 Da, the compound islikely to be immunogenic. In addition, lipids are rarely immunogenic,again unless complexed to a carrier. Astaxanthin, as a xanthophyllcarotenoid, is highly lipid soluble in natural form. It is also small insize (597 Da). Therefore, an injectable astaxanthin structural analog orderivative has a low likelihood of immunogenicity in the rightformulation, and is a particularly desirable compound for the currenttherapeutic indication.

Prevention of Arrhythmia: Pharmacologic Agents Used in Previous AnimalTrials

Studies conducted by Gutstein et al. (2001) evaluated geneticallymodified mice incapable of expressing connexin 43 in the myocardium[C×43 conditional knockout (CKO) mice]. Gutstein et al. discovered thatdespite normal heart structure and contractile performance, C×43 CKOmice uniformly developed sudden cardiac death, apparently fromspontaneous ventricular lethal tachycardia(s). This data supports thecritical role of the gap junction channel, and connexin 43 inparticular, in maintaining cardiac electrical stability. Connexin 43,which is capable of being induced by carotenoids, is the most widelyexpressed connexin in human tissues. Carotenoids, and carotenoidstructural analogs or derivatives, therefore, may be used for thetreatment of arrhythmia.

Prevention of Cancer: Pharmacologic Agents Used in Previous AnimalTrials

Carotenoids have been evaluated, mostly in animal models, for theirpossible therapeutic value in the prevention and treatment of cancer.Previously the antioxidant properties of carotenoids were the focus ofstudies directed towards carotenoids and their use in cancer prevention.Studies conducted by Bertram et al. (1991) pointed towards the fact thatalthough carotenoids were antioxidants, this particular property did notappear to be the major factor responsible for their activity as cancerchemopreventive agents. It was, however, discovered that the activity ofcarotenoids was strongly correlated with their ability to upregulate gapjunctional communication. It has been postulated that gap junctionsserve as conduits for antiproliferative signals generated bygrowth-inhibited normal cells. Connexin 43, which is capable of beinginduced by carotenoids, is the most widely expressed connexin in humantissues. Upregulation of connexin 43, therefore, may be the mechanism bywhich carotenoids are useful in the chemoprevention of cancer in humansand other animals. And recently, a human study by Nishino et al. (2003)demonstrated that a cocktail of carotenoids (10 mg lycopene, 5 mg eachof α- and β-carotene) given by chronic oral administration wasefficacious in the chemoprevention of hepatocellular carcinoma inhigh-risk cirrhotic patients in Japan. It is likely, then, that morepotent cancer-chemopreventive carotenoids (such as astaxanthin), whichaccumulate more dramatically in liver, will be particularly usefulembodiments.

Use of Carotenoids for the Treatment of Ischemia-Reperfusion Injury,Liver Disease, Arrhythmia, and Cancer

As used herein the terms “inhibiting” and “ameliorating” are generallydefined as the prevention and/or reduction of the negative consequencesof a disease state. Thus, the methods and compositions described hereinmay have value as both an acute and a chronic (prophylactic) modality.

As used herein the term “ischemia-reperfusion injury” is generallydefined as the pathology attributed to reoxygenation of previouslyischemic tissue (either chronically or acutely ischemic), which includesatherosclerotic and thromboembolic vascular disease and its relatedillnesses. In particular, major diseases or processes includingmyocardial infarction, stroke, peripheral vascular disease, venous orarterial occlusion and/or restenosis, organ transplantation, coronaryartery bypass graft surgery, percutaneous transluminal coronaryangioplasty, and cardiovascular arrest and/or death are included, butare not seen as limiting for other pathological processes which involvereperfusion of ischemic tissue in their individual pathologies.

As used herein the term “arrhythmia” is generally defined as anyvariation from the normal rhythm of the heart beat, including sinusarrhythmia, premature beat, heart block, atrial fibrillation, atrialflutter, ventricular tachycardia, ventricular fibrillation, torsades depointes, pulsus alternans and paroxysmal tachycardia. As used herein theterm “cardiac arrhythmia” is generally defined as a disturbance of theelectrical activity of the heart that manifests as an abnormality inheart rate or heart rhythm. Arrhythmia is most commonly related tocardiovascular disease, and in particular, ischemic heart disease.

As used herein the term “cancer” is generally considered to becharacterized by the uncontrolled, abnormal growth of cells. Inparticular, cancer may refer to tissue in a diseased state includingpre-cancerous, carcinogen-initiated and carcinogen-transformed cells.

As used herein the terms “structural carotenoid analogs or derivatives”may be generally defined as carotenoids and the biologically activestructural analogs or derivatives thereof. “Derivative” in the contextof this application is generally defined as a chemical substance derivedfrom another substance either directly or by modification or partialsubstitution. “Analog” in the context of this application is generallydefined as a compound that resembles another in structure but is notnecessarily an isomer. Typical analogs or derivatives include moleculeswhich demonstrate equivalent or improved biologically useful andrelevant function, but which differ structurally from the parentcompounds. Parent carotenoids are selected from the more than 700naturally-occurring carotenoids described in the literature, and theirstereo- and geometric isomers. Such analogs or derivatives may include,but are not limited to, esters, ethers, carbonates, amides, carbamates,phosphate esters and ethers, sulfates, glycoside ethers, with or withoutspacers (linkers).

As used herein the terms “the synergistic combination of more than onestructural analog or derivative of carotenoids” may be generally definedas any composition including one structural carotenoid analog orderivative combined with one or more other structural carotenoid analogsor derivatives or co-antioxidants, either as derivatives or in solutionsand/or formulations.

As used herein the terms “subject” may be generally defined as allmammals, in particular humans.

As used herein the terms “administration” may be generally defined asthe administration of the pharmaceutical or over-the-counter (OTC) ornutraceutical compositions by any means that achieves their intendedpurpose. For example, administration may include parenteral,subcutaneous, intravenous, intracoronary, rectal, intramuscular,intra-peritoneal, transdermal, or buccal routes. Alternatively, orconcurrently, administration may be by the oral route. The dosageadministered will be dependent upon the age, health, weight, and/ordisease state of the recipient, kind of concurrent treatment, if any,frequency of treatment, and/or the nature of the effect desired.

In some embodiments, techniques described herein may be applied to theinhibition and/or amelioration of any disease or disease state relatedto reactive oxygen species. Any techniques described herein directedtowards the inhibition of ischemia-reperfusion injury may also beapplied to the inhibition or amelioration of a liver disease, anon-limiting example being Hepatitis C infection. Techniques describedherein directed towards the inhibition and/or amelioration ofischemia-reperfusion injury may also be applied to the inhibition and/oramelioration of arrhythmia. Techniques described herein directed towardsthe inhibition and/or amelioration of ischemia-reperfusion injury mayalso be applied to the inhibition and/or amelioration of cancer. In someembodiments, techniques described herein may be used for controllingconnexin 43 expression. Techniques described herein may be used tocontrol gap junctional communication. In some embodiments, techniquesdescribed herein may be used for controlling C-reactive protein levels.

An embodiment may include the administration of structural carotenoidanalogs or derivatives alone or in combination to a subject such thatthe occurrence of ischemia-reperfusion injury is thereby inhibitedand/or ameliorated. The structural carotenoid analogs or derivatives maybe water soluble and/or water dispersible derivatives. The carotenoidderivatives may include any substituent that substantially increases thewater solubility of the naturally occurring carotenoid. The carotenoidderivatives may retain and/or improve the antioxidant properties of theparent carotenoid. The carotenoid derivatives may retain the non-toxicproperties of the parent carotenoid. The carotenoid derivatives may haveincreased bioavailability, relative to the parent carotenoid, uponadministration to a subject. The parent carotenoid may be naturallyoccurring.

Another embodiment may include the administration of a compositioncomprised of the synergistic combination of more than one structuralanalog or derivative of carotenoids to a subject such that theoccurrence of ischemia-reperfusion injury is thereby reduced. Thecomposition may be a “racemic” (i.e. mixture of the potentialstereoisomeric forms) mixture of carotenoid derivatives. Included aswell are pharmaceutical compositions comprised of structural analogs orderivatives of carotenoids in combination with a pharmaceuticallyacceptable carrier. In one embodiment, a pharmaceutically acceptablecarrier may be serum albumin. In one embodiment, structural analogs orderivatives of carotenoids may be complexed with human serum albumin(i.e., HSA) in a solvent. HSA may act as a pharmaceutically acceptablecarrier.

In some embodiments, compositions may include all compositions of 1.0gram or less of a particular structural carotenoid analog, incombination with 1.0 gram or less of one or more other structuralcarotenoid analogs or derivatives and/or co-antioxidants, in an amountwhich is effective to achieve its intended purpose. While individualsubject needs vary, determination of optimal ranges of effective amountsof each-component is with the skill of the art. Typically, a structuralcarotenoid analog or derivative may be administered to mammals, inparticular humans, orally at a dose of 5 to 100 mg per day referenced tothe body weight of the mammal or human being treated forischemia-reperfusion injury. Typically, a structural carotenoid analogor derivative may be administered to mammals, in particular humans,parenterally at a dose of between 5 to 1000 mg per day referenced to thebody weight of the mammal or human being treated forischemia-reperfusion injury. In other embodiments, about 100 mg of astructural carotenoid analog or derivative is either orally orparenterally administered to treat or prevent ischemia-reperfusioninjury.

The unit oral dose may comprise from about 0.25 mg to about 1.0 gram, orabout 5 to 25 mg, of a structural carotenoid analog. The unit parenteraldose may include from about 25 mg to 1.0 gram, or between 25 mg and 500mg, of a structural carotenoid analog. The unit intracoronary dose mayinclude from about 25 mg to 1.0 gram, or between 25 mg and 100 mg, of astructural carotenoid analog. The unit doses may be administered one ormore times daily, on alternate days, in loading dose or bolus form, ortitrated in a parenteral solution to commonly accepted or novelbiochemical surrogate marker(s) or clinical endpoints as is with theskill of the art.

In addition to administering a structural carotenoid analog orderivative as a raw chemical, the compounds may be administered as partof a pharmaceutical preparation containing suitable pharmaceuticallyacceptable carriers, preservatives, excipients and auxiliaries whichfacilitate processing of the structural carotenoid analog or derivativewhich may be used pharmaceutically. The preparations, particularly thosepreparations which may be administered orally and which may be used forthe preferred type of administration, such as tablets, softgels,lozenges, dragees, and capsules, and also preparations which may beadministered rectally, such as suppositories, as well as suitablesolutions for administration by injection or orally or by inhalation ofaerolsolized preparations, may be prepared in dose ranges that providesimilar bioavailability as described above, together with the excipient.While individual needs may vary, determination of the optimal ranges ofeffective amounts of each component is within the skill of the art.

The pharmaceutical preparations may be manufactured in a manner which isitself known to one skilled in the art, for example, by means ofconventional mixing, granulating, dragee-making, softgel encapsulation,dissolving, extracting, or lyophilizing processes. Thus, pharmaceuticalpreparations for oral use may be obtained by combining the activecompounds with solid and semi-solid excipients and suitablepreservatives, and/or co-antioxidants. Optionally, the resulting mixturemay be ground and processed. The resulting mixture of granules may beused, after adding suitable auxiliaries, if desired or necessary, toobtain tablets, softgels, lozenges, capsules, or dragee cores.

Suitable excipients may be fillers such as saccharides (e.g., lactose,sucrose, or mannose), sugar alcohols (e.g., mannitol or sorbitol),cellulose preparations and/or calcium phosphates (e.g., tricalciumphosphate or calcium hydrogen phosphate). In addition binders may beused such as starch paste (e.g., maize or corn starch, wheat starch,rice starch, potato starch, gelatin, tragacanth, methyl cellulose,hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/orpolyvinyl pyrrolidone). Disintegrating agents may be added (e.g., theabove-mentioned starches) as well as carboxymethyl-starch, cross-linkedpolyvinyl pyrrolidone, agar, or alginic acid or a salt thereof (e.g.,sodium alginate). Auxiliaries are, above all, flow-regulating agents andlubricants (e.g., silica, talc, stearic acid or salts thereof, such asmagnesium stearate or calcium stearate, and/or polyethylene glycol, orPEG). Dragee cores are provided with suitable coatings which, ifdesired, are resistant to gastric juices. Softgelatin capsules(“softgels”) are provided with suitable coatings, which, typically,contain gelatin and/or suitable edible dye(s). Animal component-free andkosher gelatin capsules may be particularly suitable for the embodimentsdescribed herein for wide availability of usage and consumption. Forthis purpose, concentrated saccharide solutions may be used, which mayoptionally contain gum arabic, talc, polyvinyl pyrrolidone, polyethyleneglycol (PEG) and/or titanium dioxide, lacquer solutions and suitableorganic solvents or solvent mixtures, including dimethylsulfoxide(DMSO), tetrahydrofuran (THF), acetone, ethanol, or other suitablesolvents and co-solvents. In order to produce coatings resistant togastric juices, solutions of suitable cellulose preparations such asacetylcellulose phthalate or hydroxypropylmethyl-cellulose phthalate,may be used. Dye stuffs or pigments maybe added to the tablets or drageecoatings or softgelatin capsules, for example, for identification or inorder to characterize combinations of active compound doses, or todisguise the capsule contents for usage in clinical or other studies.

Other pharmaceutical preparations which may be used orally includepush-fit capsules made of gelatin, as well as soft, thermally-sealedcapsules made of gelatin and a plasticizer such as glycerol or sorbitol.The push-fit capsules may contain the active compounds in the form ofgranules which may be mixed with fillers such as, for example, lactose,binders such as starches, and/or lubricants such as talc or magnesiumstearate and, optionally, stabilizers and/or preservatives. In softcapsules, the active compounds may be dissolved or suspended in suitableliquids, such as fatty oils such as rice bran oil or peanut oil or palmoil, or liquid paraffin. In some embodiments, stabilizers andpreservatives may be added.

In some embodiments, pulmonary administration of a pharmaceuticalpreparation may be desirable. Pulmonary administration may include, forexample, inhalation of aerosolized or nebulized liquid or solidparticles of the pharmaceutically active component dispersed in andsurrounded by a gas.

Possible pharmaceutical preparations which may be used rectally include,for example, suppositories, which consist of a combination of the activecompounds with a suppository base. Suitable suppository bases are, forexample, natural or synthetic triglycerides, or parrafin hydrocarbons.In addition, it is also possible to use gelatin rectal capsules whichconsist of a combination of the active compounds with a base. Possiblebase materials include, for example, liquid triglycerides, polyethyleneglycols, or paraffin hydrocarbons.

Suitable formulations for parenteral administration include, but are notlimited to, aqueous solutions of the active compounds in water-solubleand/or water dispersible form, for example, water-soluble salts, esters,carbonates, phosphate esters or ethers, sulfates, glycoside ethers,together with spacers and/or linkers. Suspensions of the activecompounds as appropriate oily injection suspensions may be administered,particularly suitable for intramuscular injection. Suitable lipophilicsolvents, co-solvents (such as DMSO or ethanol), and/or vehiclesincluding fatty oils, for example, rice bran oil or peanut oil and/orpalm oil, or synthetic fatty acid esters, for example, ethyl oleate ortriglycerides, may be used. Aqueous injection suspensions may containsubstances which increase the viscosity of the suspension including, forexample, sodium carboxymethyl cellulose, sorbitol, dextran, and/orcyclodextrins. Cyclodextrins (e.g., β-cyclodextrin) may be usedspecifically to increase the water solubility for parenteral injectionof the structural carotenoid analog. Liposomal formulations, in whichmixtures of the structural carotenoid analog or derivative with, forexample, egg yolk phosphotidylcholine (E-PC), may be made for injection.Optionally, the suspension may contain stabilizers, for example,antioxidants such as BHT, and/or preservatives, such as benzyl alcohol.

EXAMPLES

Having now described the invention, the same will be more readilyunderstood through reference to the following example(s), which areprovided by way of illustration, and are not intended to be limiting ofthe present invention.

Regarding the synthesis and characterization of compounds describedherein, reagents were purchased from commercial sources and used asreceived unless otherwise indicated. Solvents for reactions andisolations were reagent grade and used without purification unlessotherwise indicated. All of the following reactions were performed undernitrogen (N₂) atmosphere and were protected from direct light. “Racemic”astaxanthin (as the mixture of stereoisomers 3S,3′S, meso, and 3R,3′R ina 1:2:1 ratio) was purchased from Divi's Laboratories, Ltd (BucktonScott, India). “Racemic” lutein and zeaxanthin were purchased fromIndofine Chemical Co., Inc. Thin-layer chromatography (TLC) wasperformed on Uniplate Silica gel GF 250 micron plates. HPLC analysis forin-process control (IPC) was performed on a Varian Prostar Series 210liquid chromatograph with an AIltech Rocket, Platinum-C18, 100 ÅA, 3 μm,7×53 mm, PN 50523; Temperature: 25° C.; Mobile phase: (A=water; B=10%dichloromethane/methanol), 40% A/60% B (start); linear gradient to 100%B over 8 min; hold 100% B over 4 min, linear gradient to 40% A/60% Bover 1 min; Flow rate: 2.5 ml/min; Starting pressure: 2050 PSI; PDADetector wavelength: 474 nm. NMR was recorded on a Bruker Advance 300and mass spectroscopy was taken on a ThermoFinnigan AQA spectrometer.LC/MS was recorded on an Agilent 1100 LC/MSD VL ESI system; column:Zorbax Eclipse XDB-C18 Rapid Resolution (4.6×75 mm, 3.5 μm, USUT002736);temperature: 25° C.; starting pressure: 107 bar; flow rate: 1.0 ml/min.;mobile phase (% A=0.025% TFA in H₂O, % B=0.025% TFA in acetonitrile)Method 1 (compounds 8-21, 23-27, 30, 31): 70% A/30% B (start), stepgradient to 50% B over 5 min., step gradient to 98% B over 8.30 min.,hold at 98% B over 15.20 min., step gradient to 30% B over 15.40 min.;Method 2 (compounds 28, 29): 70% A/30% B (start), step gradient to 50% Bover 4 min., step gradient to 90% B over 7.30 min., step gradient to 98%B over 10.30 min., hold at 98% B over 15.20 min., step gradient to 30% Bover 15.40 min.; Method 3 (compound 22): 70% A/30% B (start), stepgradient to 50% B over 5 min., step gradient to 98% B over 8.30 min.,hold at 98% B over 25.20 min., step gradient to 30% B over 25.40 min.;PDA Detector: 470 nm; LRMS:+mode, ESI.

Astaxanthin (2E). HPLC retention time: 11.629 min., 91.02% (AUC); LRMS(ESI) m/z (relative intensity): 598 (M⁺+2H) (60), 597 (M⁺+H) (100); HPLCretention time: 12.601 min., 3.67% (AUC); LRMS (ESI) m/z (relativeintensity): 597 (M⁺+H) (100); HPLC retention time: 12.822 min., 5.31%(AUC); LRMS (ESI) m/z (relative intensity): 597 (M⁺+H) (100).

Lutein (XXX). HPLC retention time: 12.606 min., 100% (AUC); LRMS (ESI)m/z (relative intensity): 568 (M⁺) (100).

Zeaxanthin (XXXI). HPLC retention time: 12.741 min., 100% (AUC); LRMS(EST) m/z (relative intensity): 568 (M⁺) (100).

EXAMPLE 1

Synthesis of XV (the Disuccinic Acid ester of Astaxanthin (Succinic acidmono-(4-{18-[4-(3-carboxy-propionyloxy)-2,6,6-trimethyl-3-oxo-cyclohex-1-enyl]-3,7,12,16-tetramethyl-octadeca-1,3,5,7,9,11,13,15,17-nonaenyll}-3,5,5-trimethyl-2-oxo-cyclohex-3-enyl)ester))

To a solution of astaxanthin 2E (6.0 g, 10.05 mmol) in DCM(“dichloromethane”) (50 mL) at room temperature was added DIPEA(“N,N-diisopropylethylamine”) (35.012 mL, 201 mmol), succinic anhydride(10.057 g, 100.5 mmol), and DMAP (“4-(dimethylamino)pyridine”) (0.6145g, 5.03 mmol). The reaction mixture was stirred at room temperature for48 hours, at which time the reaction was diluted with DCM, quenched withbrine/1M HCl (60 mL/10 mL), and then extracted with DCM. The combinedorganic layers were dried over Na₂SO₄ and concentrated to yieldastaxanthin disuccinate (XV) (100%) HPLC retention time: 10.031 min.,82.57% (AUC); LRMS (ESI) m/z (relative intensity): 798 (M⁺+2H) (52), 797(M⁺+H) (100); HPLC retention time: 10.595 min., 4.14% (AUC); LRMS (ESI)m/z (relative intensity): 797 (M⁺+H) (40), 697 (100); HPLC retentiontime: 10.966 min., 5.68% (AUC); LRMS (ESI) m/z (relative intensity): 797(M⁺+H) (100), 679 (31); HPLC retention time: 11.163 min., 7.61% (AUC);LRMS (ESI) m/z (relative intensity): 797 (M⁺+H) (38), 679 (100), and nodetectable astaxanthin 2E.

Example 2 Synthesis of XVI (the Disodium Salt of the Disuccinic Acidester of Astaxanthin (Succinic acidmono-(4-{18-[4-(3-carboxy-propionyloxy)-2,6,6-trimethyl-3-oxo-cyclohex-1-enyl]-3,7,12,16-tetramethyl-octadeca-1,3,5,7,9,11,13,15,17-nonaenyl}-3,5,5-trimethyl-2-oxo-cyclohex-3-enyl)ester))

Disuccinic acid ester of astaxanthin XV (2 g, 2.509 mmol) and 200 mLethanol were stirred at room temperature under nitrogen in a 500 mLround-bottom flask. Sodium ethoxide (340 mg, 5.019 mmol, Acros#A012556101) was added as a solid in a single portion and the solutionwas allowed to stir overnight. The following day, the precipitate wasfiltered off and washed with ethanol followed by methylene chloride toafford a purple solid, the disodium salt of the disuccinic acid ester ofastaxanthin, XVI [1.41 g, 67%] and was placed on a high vacuum line todry. ¹H-NMR (Methanol-d₄) δ 6.77-6.28 (14 H, m), 5.53 (2 H, dd, J=12.6,6.8), 2.68-2.47 (8 H, m), 2.08-1.88 (22 H, m), 1.37 (6 H, s), 1.24 (6H,s); ¹³C NMR (CDCl₃) δ 196.66, 180.80, 175.01, 163.69, 144.12, 141.38,138.27, 136.85, 136.12, 135.43, 132.35, 129.45, 126.22, 124.71, 72.68,44.09, 38.63, 34.02, 32.34, 31.19, 26.86, 14.06, 13.19, 12.91; Massspectroscopy+ESI, 819.43 monosodium salt, 797.62 disuccinic acid esterof astaxanthin XV; HPLC 7.41 min (99.84%).

Example 3 Synthesis of the BocLys(Boc)OH ester of Astaxanthin (XXI)

HPLC: Column: Waters Symmetry C18 3.5 micron 4.6 mm×150 mm; Temperature:25° C.; Mobile phase: (A=0.025 % TFA in H₂O; B=0.025% TFA in MeCN), 95%A/5% B (start); linear gradient to 100% B over 12 min, hold for 4 min;linear gradient to 95% B/5% A over 2 min; linear gradient to 95% A/5% Bover 4 min; Flow rate: 2.5 mL/min; Detector wavelength: 474 nm.

To a mixture of astaxanthin 2E (11.5 g, 19.3 mmol) and BocLys(Boc)OH(20.0 g, 57.7 mmol) in methylene chloride (500 mL) were added4-dimethylaminopyridine (DMAP) (10.6 g, 86.6 mmol) and1,3-diisopropylcarbodiimide (“DIC”) (13.4 g, 86.7 mmol). Theround-bottomed flask was covered with aluminum foil and the mixture wasstirred at ambient temperature under nitrogen overnight. After 16 hours,the reaction was incomplete by HPLC and TLC. An additional 1.5equivalents of DMAP and DIC were added to the reaction and after 2hours, the reaction was complete by HPLC. The mixture was thenconcentrated to 100 mL and a white solid (1,3-diisopropylurea) wasfiltered off. The filtrate was flash chromatographed through silica gel(10% to 50% Heptane/EtOAc) to give the desired product as a dark redsolid (XXI) (28.2 g,>100% yield). ¹H NMR (DMSO-d₆) δ 7.24 (2 H, t, J=6.3Hz), 6.78 (2 H, d, 5.0 Hz), 6.57-6.27 (14 H, m), 5.50-5.41 (2 H, m),3.99-3.97 (2 H, d, 6.0 Hz), 2.90 (4 H, m), 2.03 (4 H, m), 2.00 (6 H, s),1.97 (6 H, s), 1.82 (6 H, s), 1.70-1.55 (4 H, m), 1.39-1.33 (36 H, m),1.24-1.13 (8 H, m), 1.01-0.99 (6 H, m), 0.86-0.83 (6 H, m). HPLC: 21.3min (24.6% AUC)); 22.0 min (48.1% (AUC)); 22.8 min (20.6% (AUC)). TLC(1:1 Heptane/EtOAc: R_(f)0.41; R_(f)0.5; R_(f)0.56). LC/MS analysis wasperformed on a Agilent 1100 LC/MSD VL ESI system by flow injection inpositive mode; Mobile Phase: A=0.025% TFA in H₂O; B=0.025% TFA in MeCN,10% A/90% B(start); Starting pressure: 10 bar; PDA Detector 470 nm.+ESI, m/z=1276.1(M+Na⁺).

Example 4 Synthesis of the Tetrahydrochloride Salt of the Dilysinateester of Astaxanthin (XX)

A mixture of DiBocLys(Boc) ester of astaxanthin (XXI) (20.0 g, 16.0mmol) and HCl in 1,4-dioxane (4.00 M, 400 mL, 1.60 mol, 100 eq) wasstirred at ambient temperature under a nitrogen atmosphere. Theround-bottomed flask was covered with aluminum foil and the reaction wasstirred for 1 hour, at which time the reaction was complete by HPLC. Thetitle compound XX precipitated and was collected by filtration, washedwith ether (3×100 mL) and dried (14.7 g, 92%, 91.6% purity by HPLC). Aportion (13.5 g) of the crude solid was dissolved in 500 mL of a 1:2methanol/methylene chloride mixture and stirred under nitrogen. Diethylether (168 mL) was then added dropwise and the precipitated solid wascollected by filtration to afford the desired product XX as a dark redsolid (8.60 g, 63.7% yield). ¹H NMR (DMSO-d₆) δ 8.65 (6 H, s), 8.02 (6H, s), 6.78-6.30 (14 H, m), 5.59-5.51 (2 H, m), 4.08 (2 H, m), 2.77 (4H, m), 2.09-2.07 (4 H, m), 2.01 (6 H, s), 1.97 (6 H, s), 1.90-1.86 (4 H,m), 1.84 (6 H, s), 1.61-1.58 (8 H, m), 1.37 (6 H, s), 1.22 (6 H, s).HPLC: 7.8 min (97.0% (AUC)). LC/MS analysis was performed on an Agilent1100 LC/MSD VL ESI system with Zorbax Eclipse XDB-C18 Rapid Resolution4.6×75 mm, 3.5 microns, USUT002736; Temperature: 25° C.; Mobile Phase:(% A=0.025% TFA in H₂O; % B=0.025% TFA in MeCN), 70% A/30% B (start);linear gradient to 50% B over 5 min, linear gradient to 100% B over 7min; Flow rate: 1.0 mL/min; Starting pressure: 108 bar; PDA Detector 470nm. Mass spectrometry +ESI, m/z=853.9(M+H⁺), m/z=875.8(M+Na⁺); LC 4.5min.

Example 5 Synthesis of the Bis-(2-OTBS Ascorbic Acid) 6-Ester ofAstaxanthin Disuccinate (XXII)

HPLC: Column: Waters Symmetry C18 3.5 micron 4.6 mm×150 mm; Temperature:25° C.; Mobile phase: (A=0.025% TFA in water; B=0.025% TFA inacetonitrile), 95% A/5% B (start); linear gradient to 100% B over 5 min,hold for 10 min; linear gradient to 95% B over 2 min; linear gradient to95% A/5% B over 3 min; Flow rate: 1.0 mL/min; Detector wavelength: 474nm.

To a stirring solution of astaxanthin disuccinate (XV) (20.00 g, 25.1mmol) in 600 mL of dichloromethane was added 4-dimethylaminopyridine(DMAP) (6.13 g, 50.2 mmol), 2-O-tert-butyldimethylsilyl (OTBS) ascorbicacid (XXVI) (21.86 g, 75.3 mmol), and1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI-HCl)(12.02 g, 62.75 mmol). After 14 h, the reaction mixture was flashchromatographed through silica gel (1.0 kg silica gel, eluent 0.5%HOAc/5% MeOH/EtOAc). Fraction 10 was concentrated to afford dark redsolid (6.47 g, 19.2% yield, 58% AUC purity by HPLC). The crude productXXII was flashed chromatographed through silica gel (600 g silica gel,eluent 0.25% HOAc/5% MeOH/EtOAc). Fractions 6-10 were concentrated undervacuum to afford dark red solid (1.50 g, 4.4% yield, 94.8% AUC purity byHPLC ¹H-NMR (CDCl₃) δ 11.13 (2H, s), 6.78-6.28 (14 H, m), 5.43 (2 H, dd,J=12.2, 7.1 Hz), 5.34 (2H, s), 4.78 (2H, d, J=5.4 Hz), 4.11-4.07 (6H,m), 2.69-2.65 (8 H, m), 2.05-1.97 (22H, m), 1.81 (6 H, s), 1.33 (6 H,s), 0.92 (18 H, s), 0.15 (6 H, s), 0.14 (6H, s); HPLC 13.4 min [94.8%(AUC)]; Mass spectroscopy −ESI, m/z=1340.6 (M⁻).

Example 6 Synthesis of the Bis-Ascorbic Acid 6-Ester of AstaxanthinDisuccinate (XIX)

To a stirring solution of the bis-(2-OTBS ascorbic acid) 6-ester ofastaxanthin disuccinate (XXII) (100 mg, 0.075 mmol) in THF (5 mL) at 0°C. was added HF·Et₃N (121 μL, 0.745 mmol). The reaction was stirred for1 h at 0° C. then warmed to rt. The reaction was stirred 2.5 h beforebeing quenched by pouring into a separatory funnel containing 5 mL IPACand 5 mL of water. The aqueous layer was removed and the organic layerwas washing with water (2×5 mL). The organic solvents were removed byrotary evaporation to give a dark red solid XIX, which was used withoutpurification. ¹H-NMR (CDCl₃) δ 11.12 (2 H, s), 8.40 (2 H, s), 6.87-6.28(14 H, m), 5.43-5.32 (4 H, m), 4.69 (s, 2H), 4.09 (s, 4H), 3.99 (s, 2H),2.68-2.50 (m, 8H), 2.00-1.76 (22 H, m), 1.36-1.19 (12 H, m); HPLC 8.9min [80.7% (AUC)]; Mass spectroscopy +ESI, m/z=1113.2 (M+H⁺).

Example 7 Synthesis of the Sodium Salt of the Bis-Ascorbic Acid 6-Esterof Astaxanthin Disuccinate (XXIII)

To a stirring solution of the crude bis-ascorbic acid 6-ester ofastaxanthin disuccinate (XIX) (0.075 mmol) in acetone (5 mL) at rt wasadded triethylorthoformate (62 μL, 0.373 mmol). The solution was stirred15 min then a solution of sodium 2-ethylhexanoate in acetone (93 μL,0.019 mmol, 0.20 M) was added dropwise. The resulting precipitate wasremoved by filtration. The filtrate was cooled to 0° C. and treated withadditional sodium 2-ethylhexanoate in acetone (373 μL, 0.075 mmol, 0.20M). The reaction was stirred for 5 min then the solid material wascollected by filtration, washed with acetone (5 mL), and dried underhigh vacuum to give a dark red solid XXIII (27.8 mg, 32.2% yield): HPLC8.9 min [88.2% (AUC)], Mass spectroscopy +APCI, m/z=1113.3 (M+3H−2Na⁺).

Example 8 Synthesis of the Dicyclohexylmethyl Ester of AstaxanthinDisuccinate (XXIV)

HPLC: Column: Alltech Rocket, Platinum-C18, 100 ÅA, 3 μm, 7×53 mm;Temperature: 25° C.; Mobile phase: (A=0.025% TFA in water; B=0.025% TFAin acetonitrile), 70% A/30% B (start); hold for 40 sec; linear gradientto 50% B over 4 min 20 sec; linear gradient to 100% B over 1 min 30 sec,hold for 4 min 40 sec; linear gradient to 70% A/30% B in 20 sec; Flowrate: 2.5 mL/min; Detector wavelength: 474 nm.

To a stirred solution of the astaxanthin disuccinate (XV) (100 mg, 0.125mmol) and N,N-dimethylformamide (6.0 mL) in a 25 mL round-bottom flaskwas added cesium carbonate (90.0 mg, 0.275 mmol) at room temperatureunder N₂ and covered with aluminum foil. The reaction was stirred for 15minutes then bromomethyl cyclohexane (52.0 μL, 0.375 mmol) was added.After 2 days, the reaction was quenched by adding 4 mL of a saturatedsolution of sodium bicarbonate and diluted with 50 mL ofdichloromethane. The diluted solution was washed twice with 25 mL ofwater before drying over anhydrous sodium sulfate. The organic solutionwas filtered and the solvent was removed by rotary evaporation. Thecrude residue was purified by flash chromatography (10-50%EtOAc/heptane) to afford a dark red solid XXIV (40.2 mg, 32.5% yield):¹H-NMR (CDCl₃) δ 7.03-6.17 (14 H, m), 5.54 (2 H, dd, J=12.9, 6.7 Hz),3.92 (4 H, d, J=6.4 Hz), 2.82-2.63 (8 H, m), 2.08-1.92 (14 H, m), 1.90(6 H, s), 1.75-1.62 (14 H, m), 1.34-1.20 (22 H, m); HPLC 8.9 min [83.9%(AUC)]; TLC (3:7 EtOAc/heptane: R_(f)0.38); Mass spectroscopy +ESI,m/z=989.6 (M+H⁺).

Example 9 Synthesis of 2-OTBS-5,6-Isopropyledine Ascorbic Acid (XXV)

HPLC: Alltech Rocket, Platinum-C18, 100A, 3 μm, 7×53 mm, PN 50523;Temperature: 25° C.; Mobile phase: (A=0.025 % TFA in water; B=0.025% TFAin acetonitrile), 90% A/10% B (start); linear gradient to 30% B over 3min; linear gradient to 90% B over 3 min, hold for 2 min; lineargradient to 90% A/10% B over 1 min, then hold for 1 min; Flow rate: 2.5mumin; Detector wavelength: 256 nm.

To a stirring solution of 5,6-isopropyledine ascorbic acid (100.0 g, 463mmol) in 1.00 L THF was added tert-butyldimethylsilyl chloride (TBSCI)(76.7 g, 509 mmol) at rt followed by addition ofN,N-diisopropylethylamine (DIPEA) (161 mL, 925 mmol) over 30 min. Thereaction was stirred 14 h at rt, then concentrated under vacuum. Themixture was dissolved in methyl tert-butyl ether (MTBE) (1.00 L) andextracted with 1 M potassium carbonate (1.00 L) in a separatory funnel.The aqueous layer was extracted one more time with MTBE (1.00 L), andthe pH of the aqueous layer was adjusted to pH 6 using 2 N HCl. Theaqueous layer was extracted twice with isopropyl acetate (IPAC) (1.00 L)and concentrated to afford an off white solid XXV (150.4 g, 98% yield):¹H-NMR (DMSO d₆) δ 11.3 (1 H, s), 4.78 (1 H, d, J=2.0 Hz), 4.41-4.36 (1H, m), 4.11 (1 H, dd, J=8.4, 7.4 Hz), 3.92 (1 H, dd, J=8.4, 6.0), 1.24(3 H, s), 1.23 (3 H, s), 0.92 (9 H, s), 0.14 (6H, s); HPLC 5.9 min[91.6% (AUC)]; Mass spectroscopy −ESI, m/z=329.2 (M−H⁻).

Example 10 Synthesis of 2-OTBS Ascorbic Acid (XXVI)

To a stirring solution of 2-OTBS-5,6-isopropyledine ascorbic acid (XXV)(150.4 g, 455 mmol) in 1.50 L of dichloromethane at rt was addedpropanedithiol (54.0 mL, 546 mmol) under nitrogen. The solution wascooled to −45° C., and then BF₃—OEt₂ (58.0 mL, 455 mmol) was addeddropwise at a rate that kept the temperature below −40° C. After 1 h,the reaction was complete by HPLC. The reaction was quenched by pouringthe cold reaction mixture into a separatory funnel containing 1.00 L ofIPAC and 500 mL of a saturated solution of ammonium chloride and 500 mLof water. The organic layer was concentrated to a white solid. In orderto purge the propane dithiol, the solid was reslurried indichloromethane (250 mL) for 2 h and heptane (1.00 L) was added andstirred for 1 h. The mixture was concentrated under vacuum to a volumeof 500 mL. The mixture was filtered and dried under vacuum to afford anof white solid XXVI (112.0 g, 85% yield): ¹H-NMR (DMSO d₆) δ 11.0 (1 H,s), 4.89 (2 H, s), 4.78 (1 H, d, J=1.2 Hz), 3.82-3.80 (1 H, m),3.45-3.42 (2 H, m), 0.923 (9 H, s), 0.14 (6H, s); HPLC 4.9 min [92.0%(AUC)]; Mass spectroscopy −ESI, m/z=289.0 (M−H⁻).

Example 11 Synthesis of the bis-dimethylphosphate Ester of Astaxanthin(XXVII)

HPLC: Waters Symmetry C18, 3 μm, 4.6×150 mm, WAT200632, Temperature: 25°C.; Mobile phase: (A=water; B=10% DCM/MeOH), 10% A/90% B (start); lineargradient to 100% B over 9 min; hold 100% B over 11 min, linear gradientto 10% A/90% B over 1 min; Flow rate: 1.0 mL/min; Detector wavelength:474 nm.

To a mixture of astaxanthin 2E (500 mg, 0.84 mmol) and methyl imidazole(0.50 mL, 6.27 mmol) in methylene chloride at 37° C. was addeddimethylbromophosphate (2 M, 5.04 mL) (Ding, 2000). After 24 h, thereaction was not complete by HPLC and dimethylbromophosphate (2 M, 5.04mL) was added. After 48 h, the reaction was not complete by HPLC anddimethylbromophosphate (2 M, 5.04 mL) was added. After 72 h, thereaction was complete by HPLC. The reaction was diluted with methylenechloride (20 mL) and quenched with water (20 mL). The layers wereseparated and the aqueous layer was extracted again with 20 mL ofmethylene chloride. The organic layers were combined and concentratedunder vacuum to afford 2.69 g (>100% yield) of XXVII. ¹H NMR (CDCl₃) δ6.58-6.14 (14 H, m), 5.05-4.95 (2 H, m), 3.91-3.60 (12 H, m), 2.11-2.04(4 H, m), 2.04-1.92 (12 H, m), 1.85 (6 H, s), 1.26 (6 H, s), 1.15 (6 H,s). HPLC: 4.29 min (86.7% AUC)). Mobile Phase: A=0.025% TFA in H₂O;B=0.025% TFA in acetonitrile, 10% A/90% B(start); PDA Detector 474 nm.+ESI, m/z=813.62 (M+1).

Example 12 Synthesis of the BocProOH ester of Astaxanthin (XXVIII

LC/MS Analysis: LC/MS analysis was performed on an Agilent 1100 LC/MSDVL ESI system with Zorbax Eclipse XDB-C18 Rapid Resolution 4.6×75 mm,3.5 μm, USUT002736; Temperature: 25° C.; Mobile Phase:(% A=0.025% TFA inH₂O; % B=0.025% TFA in MeCN), 70% A/30% B(start); linear gradient to 50%B over 5 min, linear gradient to 98% B over 3 min, hold at 98% B for 17min; Flow rate: 1.0 mL/min; Starting pressure: 108 bar; PDA Detector 470nm, 373 nm, 214 nm. LRMS: +mode, ESI.

To a mixture of astaxanthin 2E (5.00 g, 8.38 mmol) and BocProOH (10.8 g,50.3 mmol) in methylene chloride (500 mL) were added4-dimethylaminopyridine (DMAP) (6.14 g, 50.3 mmol) and1,3-diisopropylcarbodiimide (DIC) (7.79 mL, 50.3 mmol). The mixture wasstirred at ambient temperature under nitrogen overnight. After 16 hours,the reaction was complete by TLC. The mixture was then concentrated todryness and the crude residue was slurried with 100 mL of diethyl etherand filtered through a pad of Celite. The filtrate was flashchromatographed through silica gel (Et₂O) to give the desired productXXVIII as a dark red solid (8.56 g, >100% yield). LC: 17.5 min [23.1%AUC)]; 18.2 min [45.1% (AUC)]; 19.4 min [22.0% (AUC)]. TLC (3:2EtOAc/Hexane: R_(f) 0.51; R_(f) 0.55; R_(f) 0.59). MS +ESI, m/z=1013.8(M+Na⁺).

EXAMPLE 13 Synthesis of the Dihydrochloride Salt of the DiprolinateEster of Astaxanthin (XMX)

A mixture of diethyl ether (130 mL) and EtOH (48.9 mL, 838 mmol) wascooled to −78° C. under a nitrogen atmosphere. Acetyl chloride (82.0 mL,838 mmol) was added dropwise to the cooled mixture over 30 minutes. Thereaction was removed from the cooling bath and allowed to slowly warm toroom temperature. The contents of the flask were poured into a separateround-bottomed flask containing DiBocPro ester of astaxanthin (XXVIII)(8.31 g, 8.38 mmol) and a stirrer bar. The flask was covered withaluminum foil and the reaction was stirred at ambient temperature undernitrogen overnight. After 16 hours the reaction was complete by LC. Thetitle compound XXIX precipitated and was collected by filtration, washedwith ether (3×100 mL) and dried (6.37 g, 88.0% crude yield, 75.2% purityby LC). LC: 8.00 min [75.2% (AUC)]. MS+ESI, m/z=791.7 (M+H⁺).

EXAMPLE 14 Synthesis of Lutein Disuccinate (XXXII)

To a solution of lutein (XXX) (0.010 g, 0.018 mmol) in DCM (2 mL) atroom temperature was added DIPEA (0.063 mL, 0.360 mmol), succinicanhydride (0.036 g, 0.360 mmol), and DMAP (0.021 g, 0.176 mmol). Thereaction mixture was stirred at room temperature for 48 hours, at whichtime the reaction was diluted with DCM, quenched with brine/1M HCl (6mL/1 mL), and then extracted with DCM. The combined organic layers weredried over Na₂SO₄ and concentrated to yield lutein disuccinate (XXXII)(93.09%) HPLC retention time: 11.765 min., 93.09% (AUC); LRMS (ESI) m/z(relative intensity): 769 (M⁺) (24), 651 (100), and no detectable luteinXXX.

EXAMPLE 15 Synthesis of Succinic Acid Esters of Zeaxanthin (XXXIII,XXXIV)

To a solution of zeaxanthin (XXXI) (0.010 g, 0.018 mmol) in DCM (2 mL)at room temperature was added DIPEA (0.063 mL, 0.360 mmol), succinicanhydride (0.036 g, 0.360 mmol), and DMAP (0.021 g, 0.176 mmol). Thereaction mixture was stirred at room temperature for 48 hours, at whichtime the reaction was diluted with DCM, quenched with brine/1M HCl (6mL/1 mL), and then extracted with DCM. The combined organic layers weredried over Na₂SO₄ and concentrated to yield zeaxanthin monosuccinate(XXXIII) (2.86%) HPLC retention time: 12.207 min., 2.86% (AUC); LRMS(ESI) m/z (relative intensity): 669 (M⁺+H) (53), 668 (M⁺) (100),zeaxanthin disuccinate (XXXIV) (97.14%) HPLC retention time: 11.788min., 67.42% (AUC); LRMS (ESI) m/z (relative intensity): 792 (M⁺+Na)(42), 769 (M⁺) (73), 651 (100); HPLC retention time: 13.587 min., 11.19%(AUC); LRMS (ESI) m/z (relative intensity): 792 (M⁺+Na) (36), 769 (M⁺)(38), 663 (100); HPLC retention time: 13.894 min., 18.53% (AUC); LRMS(ESI) m/z (relative intensity): 769 (M⁺) (62), 663 (77), 651 (100), andno detectable zeaxanthin XXXI.

EXAMPLE 16 Synthesis of Aconitic Acid Esters of Astaxanthin (XXXV,XXXVI)

To a solution of astaxanthin 2E (0.100 g, 0.168 mmol) in DCM/DMF(“N,N-dimethylformamide”) (4 mL/2 mL) at room temperature was addedDIPEA (0.878 mL, 5.04 mmol), cis-aconitic anhydride (0.2622 g, 1.68mmol), and DMAP (0.4105 g, 3.36 mmol). The reaction mixture was stirredat room temperature for 36 hours, at which time the reaction was dilutedwith DCM, quenched with brine/1M HCl (20 mL/3 mL), and then extractedwith DCM. The combined organic layers were concentrated to yieldaconitic monoester (XXXV) (13.25%) HPLC retention time: 10.485 min.,4.95% (AUC); LRMS (ESI) m/z (relative intensity): 777 (M⁺+Na+2H) (57),623 (100); HPLC retention time: 10.722 min., 8.30% (AUC); LRMS (ESI) m/z(relative intensity): 777 (M⁺+Na+2H) (6), 709 (100), aconitic diester(XXXVI) (27.67%) HPLC retention time: 9.478 min., 15.44% (AUC); LRMS(ESI) m/z (relative intensity): 933 (M⁺+Na+2H) (10), 831 (100); HPLCretention time: 9.730 min., 12.23% (AUC); LRMS (ESI) m/z (relativeintensity): 913 (M⁺+4H) (4), 843 (100), and astaxanthin 2E (44.40%).

EXAMPLE 17 Synthesis of Citric Acid Esters of Astaxanthin (XXXVII,XXXVIII)

To a suspension of citric acid (0.5149 g, 2.86 mmol) in DCM (8 mL) atroom temperature was added DIPEA (1.167 mL, 0.6.70 mmol), DIC (0.525 mL,3.35 mmol), DMAP (0.4094 g, 3.35 mmol), and astaxanthin 2E (0.200 g,0.335 mmol). The reaction mixture was stirred at room temperature for 36hours, at which time the reaction was diluted with DCM, quenched withbrine/1M HCl (20 mL/3 mL), and then extracted with DCM. The combinedorganic layers were concentrated to yield citric acid monoester (XXXVII)(26.56%) HPLC retention time: 9.786 min., 17.35% (AUC); LRMS (ESI) m/z(relative intensity): 773 (M⁺+3H) (14), 771 (M⁺+H) (100); HPLC retentiontime: 9.989 min., 9.21% (AUC); LRMS (ESI) m/z (relative intensity): 773(M⁺+3H) (50), 771 (M⁺+H) (100), citric acid diester (XXXVIII) (7.81%)HPLC retention time: 8.492 min., 3.11% (AUC); LRMS (ESI) m/z (relativeintensity): 968 (M⁺+Na) (75), 967 (100), 946 (M⁺+H) (37); HPLC retentiontime: 8.708 min., 2.43% (AUC); LRMS (ESI) m/z (relative intensity): 968(M⁺+Na) (95), 946 (M⁺+H) (100); HPLC retention time: 8.952 min., 2.27%(AUC); LRMS (ESI) m/z (relative intensity): 946 (M⁺+H) (19), 500 (100),and astaxanthin 2E (21.26%).

EXAMPLE 18 Synthesis of Dimethylaminobutyric Acid Monoester ofAstaxanthin (XXXIX)

To a suspension of 4-(dimethylamino)-butyric acid hydrochloride (0.2816g, 1.68 mmol) in DCM/DMF (3 mL/3 mL) at room temperature was added DIPEA(0.878 mL, 5.04 mmol), HOBT (“1-hydroxybenzotriazole”)-H₂O (0.3094 g,2.02 mmol), DMAP (0.4105 g, 3.36 mmol), and astaxanthin 2E (0.100 g,0.168 mmol). The reaction mixture was stirred at room temperature for 36hours, at which time the reaction was diluted with DCM, quenched withbrine/1M HCl (20 mL/3 mL), and then extracted with DCM. The combinedorganic layers were concentrated to yield 4-(dimethylamino)butyric acidmonoester (XXXIX) (24.50%) HPLC retention time: 9.476 min., 20.32%(AUC); LRMS (ESI) m/z (relative intensity): 732 (M⁺+Na) (13), 729 (100);HPLC retention time: 9.725 min., 4.18% (AUC); LRMS (ESI) m/z (relativeintensity): 732 (M⁺+Na) (50), 729 (100), and astaxanthin (61.21%).

EXAMPLE 19 Synthesis of Glutathione Monoester of Astaxanthin (L)

To a suspension of reduced glutathione (0.5163 g, 1.68 mmol) in DCM/DMF(3 mL/3 mL) at room temperature was added DIPEA (0.878 mL, 5.04 mmol),HOBT-H₂O (0.3094 g, 2.02 mmol), DMAP (0.4105 g, 3.36 mmol), DIC (0.316mL, 2.02 mmol), and astaxanthin 2E (0.100 g, 0.168 mmol). The reactionmixture was stirred at room temperature for 36 hours, at which time thereaction was diluted with DCM, quenched with brine/1M HCl (20 mL/3 mL),and then extracted with DCM. The combined organic layers wereconcentrated to yield glutathione monoester (L) (23.61%) HPLC retentiontime: 9.488 min., 16.64% (AUC); LRMS (ESI) m/z (relative intensity): 886(M⁺) (13), 810 (54), 766 (100); HPLC retention time: 9.740 min., 3.57%(AUC); LRMS (ESI) m/z (relative intensity): 886 (M⁺) (24), 590 (78), 546(100); HPLC retention time: 9.997 min., 3.40% (AUC); LRMS (ESI) m/z(relative intensity): 886 (M⁺) (25), 869 (85), 507 (100), andastaxanthin (68.17%).

EXAMPLE 20 Synthesis of Tartaric Acid Diester of Astaxanthin (LI)

To a suspension of (L)-tartaric acid (0.4022 g, 2.68 mmol) in DCM/DMF (5mL/5 mL) at room temperature was added DIPEA (1.167 mL, 0.6.70 mmol),HOBT-H₂O (0.5131 g, 3.35 mmol), DMAP (0.4094 g, 3.35 mmol), andastaxanthin 2E (0.200 g, 0.335 mmol). The reaction mixture was stirredat room temperature for 36 hours, at which time the reaction was dilutedwith DCM, quenched with brine/1M HCl (20 mL/3 mL), and then extractedwith DCM. The combined organic layers were concentrated to yieldtartaric acid diester (LI) (18.44%) HPLC retention time: 9.484 min.,14.33% (AUC); LRMS (ESI) m/z (relative intensity): 884 (M⁺+Na+H) (100),815 (72), 614 (72); HPLC retention time: 9.732 min., 4.11% (AUC); LRMS(ESI) m/z (relative intensity): 883 (M⁺+Na) (100), 539 (72), andastaxanthin 2E (67.11%).

EXAMPLE 21 Synthesis of Sorbitol Monoester of Astaxanthin Disuccinate(LII)

To a solution of astaxanthin disuccinate (XV) (0.200 g, 0.251 mmol) inDMF (10 mL) at room temperature was added DIPEA (1.312 mL, 7.53 mmol),HOBT-H₂O (0.4610 g, 3.01 mmol), DMAP (0.6133 g, 5.02 mmol), and(D)-sorbitol (0.4572 g, 2.51 mmol). The reaction mixture was stirred atroom temperature for 36 hours, at which time the reaction was dilutedwith DCM, quenched with brine/1M HCl (20 mL 3 mL), and then extractedwith DCM. The combined organic layers were concentrated to yieldsorbitol monoester (LII) (3.52%) HPLC retention time: 9.172 min., 3.52%(AUC); LRMS (ESI) m/z (relative intensity): 984 (M⁺+Na) (28), 503 (100),and astaxanthin disuccinate XV (91.15%).

EXAMPLE 22 Synthesis of Sorbitol Diester of Astaxanthin Disuccinate(LII)

To a solution of astaxanthin disuccinate (XV) (0.100 g, 0.125 mmol) inDCM/DMF (3 mL/3 mL) at room temperature was added DIPEA (0.656 mL, 3.76mmol), HOBT-H₂O (0.2313 g, 1.51 mmol), DMAP (0.3067 g, 2.51 mmol), DIC(0.236 mL, 1.51 mmol), and (D)-sorbitol (0.2286 g, 1.25 mmol). Thereaction mixture was stirred at room temperature for 36 hours, at whichtime the reaction was diluted with DCM, quenched with brine/1M HCl (20mL/3 mL), and then extracted with DCM. The combined organic layers wereconcentrated to yield sorbitol diester (LIII) (44.59%) HPLC retentiontime: 8.178 min., 11.58% (AUC); LRMS (ESI) m/z (relative intensity):1148 (M⁺+Na) (40), 545 (100); HPLC retention time: 8.298 min., 33.01%(AUC); LRMS (ESI) m/z (relative intensity): 1148 (M⁺+Na) (20), 545(100), and no detectable astaxanthin disuccinate XV.

EXAMPLE 23 Synthesis of Morpholine Carbamates of Astaxanthin (LIV, LV)

To a solution of astaxanthin 2E (0.100 g, 0.168 mmol) in DCM/DMF (3 mL/3mL) at room temperature was added DIPEA (0.878 mL, 5.04 mmol), DMAP(0.4105 g, 3.36 mmol), and 4-morpholine carbonyl chloride (0.196 mL,1.68 mmol). The reaction mixture was stirred at room temperature for 36hours, at which time the reaction was diluted with DCM, quenched withbrine/1M HCl (20 mL/3 mL), and then extracted with DCM. The combinedorganic layers were concentrated to yield 4-morpholine monocarbamate(LIV) (33.17%) HPLC retention time: 11.853 min., 29.01% (AUC); LRMS(ESI) m/z (relative intensity): 710 (M⁺) (100); HPLC retention time:13.142 min., 1.37% (AUC); LRMS (ESI) m/z (relative intensity): 710 (M⁺)(100); HPLC retention time: 13.383 min., 2.79% (AUC); LRMS (ESI) m/z(relative intensity): 710 (M⁺) (100), 4-morpholine dicarbamate (LV)(33.42%) HPLC retention time: 12.049 min., 29.71% (AUC); LRMS (ESI) m/z(relative intensity): 824 (M⁺+H) (54), 823 (M⁺) (100); HPLC retentiontime: 13.761 min., 1.29% (AUC); LRMS (ESI) m/z (relative intensity): 823(M⁺) (100), 692 (75); HPLC retention time: 14.045 min., 2.42% (AUC);LRMS (ESI) m/z (relative intensity): 823 (M⁺) (100), 692 (8), andastaxanthin 2E (22.10%).

EXAMPLE 24 Synthesis of Mannitol Monocarbonate of Astaxanthin (LVII)

To a solution of astaxanthin 2E (0.100 g, 0.168 mmol) in DCM (4 mL) at0° C. was added DIPEA (0.585 mL, 3.36 mmol), and1,2,2,2-tetrachloroethyl chloroformate (0.103 mL, 0.672 mmol). Thereaction mixture was stirred at 0° C. for 2 hours, then at roomtemperature for 1.5 hours, at which time (D)-mannitol (0.3060 g, 1.68mmol), DMF (3 mL), and DMAP (0.2052 g, 1.68 mmol) were added to thereaction. The reaction mixture was stirred at room temperature for 24hours, at which time the reaction was diluted with DCM, quenched withbrine (20 mL), and then extracted with DCM. The combined organic layerswere concentrated to yield mannitol monocarbonate (LVII) (10.19%) HPLCretention time: 9.474 min., 10.19% (AUC); LRMS (ESI) m/z (relativeintensity): 827 (M⁺+Na) (50), 804 (M⁺) (25), 725 (58), 613 (100), andastaxanthin 2E (53.73%).

EXAMPLE 25 Synthesis of (Dimethylamino)butyric acid Diester ofAstaxanthin (LVIII)

To a suspension of 4-(dimethylamino)butyric acid hydrochloride (0.2816g, 1.68 mmol) in DCM/DMF (3 mL/3 mL) at room temperature was added DIPEA(0.878 mL, 5.04 mmol), DMAP (0.4105 g, 3.36 mmol), HOBT-H₂O (0.3094 g,2.02 mmol), DIC (0.316 mL, 2.02 mmol), and astaxanthin 2E (0.100 g,0.168 mmol). The reaction mixture was stirred at room temperature for 36hours, at which time the reaction was diluted with DCM, quenched withbrine/1M HCl (20 mL/3 mL), and then extracted with DCM. The combinedorganic layers were concentrated to yield (dimethylamino)butyric aciddiester (LVIII) (77.70%) HPLC retention time: 7.850 min., 56.86% (AUC);LRMS (ESI) m/z (relative intensity): 824 (M⁺+H) (64), 823 (M⁺) (100);HPLC retention time: 8.443 min., 3.87% (AUC); LRMS (ESI) m/z (relativeintensity): 823 (M⁺) (5), 641 (20), 520 (100); HPLC retention time:9.021 min., 16.97% (AUC); LRMS (ESI) m/z (relative intensity): 824(M⁺+H) (58), 823 (M⁺) (100), and no detectable astaxanthin 2E.

EXAMPLE 26 Synthesis of Benzyl Monoether of Astaxanthin (LIX)

To a solution of astaxanthin 2E (0.100 g, 0.168 mmol) and benzyl bromide(0.400 mL, 3.36 mmol) in DCM/DMF (3 mL/3 mL) at 0° C. was added KHMDS(“potassium bis(trimethylsilyl)amide”) (6.72 mL; 0.5M in toluene, 3.36mmol). The reaction mixture was stirred at 0° C. for 1 hour and thenallowed to warm to room temperature. The mixture was stirred at roomtemperature for 24 hours, at which time the reaction was diluted withDCM, quenched with brine/1M HCl (20 mL/3 mL), and then extracted withDCM. The combined organic layers were concentrated to yield benzylmonoether (LIX) (15.06%) HPLC retention time: 12.705 min., 15.06% (AUC);LRMS (ESI) m/z (relative intensity): 686 (M⁺) (93), 597 (100), andastaxanthin 2E (67.96%).

EXAMPLE 27 Synthesis of Mannitol Monoether of Astaxanthin (LX)

To a solution of astaxanthin 2E (0.200 g, 0.335 mmol) in DCM (15 mL) atroom temperature was added 48% HBr (10 mL) and H₂O (30 mL). The aqueouslayer was extracted with DCM and the combined organic layers were driedover Na₂SO₄ and concentrated to yield the bromide derivative ofastaxanthin as a dark red oil. To a solution of the crude bromide inDCM/DMF (6 mL/6 mL) at room temperature was added DIPEA (1.58 mL, 9.09mmol), DMAP (0.3702 g, 3.03 mmol), and (D)-mannitol (0.5520 g, 3.03mmol). The reaction mixture was stirred at room temperature for 24hours, at which time the reaction was diluted with DCM, quenched withbrine/1M HCl (20 mL/3 mL), and then extracted with DCM. The combinedorganic layers were concentrated to yield mannitol monoether (LX)(4.40%) HPLC retention time: 9.479 min., 4.40% (AUC); LRMS (ESI) m/z(relative intensity): 783 (M⁺+Na) (64), 710 (66), 653 (100), andastaxanthin 2E (79.80%).

EXAMPLE 28 Synthesis of Tris(hydroxymethyl)aminomethane Monoamide ofAstaxanthin Disuccinate (LXI)

To a solution of astaxanthin disuccinate (XV) (0.100 g, 0.125 mmol) inDCM/DMF (3 mL/3 mL) at room temperature was added DIPEA (0.653 mL, 3.75mmol), DMAP (0.3054 g, 2.50 mmol), HOBT-H₂O (0.2297 g, 1.50 mmol), andtris(hydroxymethyl)aminomethane (0.1514 g, 1.25 mmol). The reactionmixture was stirred at room temperature for 36 hours, at which time thereaction was diluted with DCM, quenched with brine/1M HCl (20 mL/3 mL),and then extracted with DCM. The combined organic layers wereconcentrated to yield tris(hydroxymethyl)aminomethane monoamide (LXI)(4.40%) HPLC retention time: 9.521 min., 3.50% (AUC); LRMS (ESI) m/z(relative intensity): 923 (M⁺+Na) (36), 900 (M⁺) (80), 560 (100); HPLCretention time: 9.693 min., 0.90% (AUC); LRMS (ESI) m/z (relativeintensity): 923 (M⁺+Na) (11), 813 (33), 500 (100), and astaxanthindisuccinate XV (84.34%).

EXAMPLE 29 Synthesis of Tris(hydroxymethyl)aminomethane Diamide ofAstaxanthin Disuccinate (LXII)

To a solution of astaxanthin disuccinate (XV) (0.100 g, 0.125 mmol) inDCM/DMF (3 mL/3 mL) at room temperature was added DIPEA (0.653 mL, 3.75mmol), DMAP (0.3054 g, 2.50 mmol), HOBT-H₂O (0.2297 g, 1.50 mmol), DIC(0.235 mL, 1.50 mmol), and tris(hydroxymethyl)aminomethane (0.1514 g,1.25 mmol). The reaction mixture was stirred at room temperature for 36hours, at which time the reaction was diluted with DCM, quenched withbrine/1M HCl (20 mL/3 mL), and then extracted with DCM. The combinedorganic layers were concentrated to yieldtris(hydroxymethyl)aminomethane diamide (LXII) (66.51%) HPLC retentiontime: 8.086 min., 19.34% (AUC); LRMS (ESI) m/z (relative intensity):1026 (M⁺+Na) (22), 1004 (M⁺+H) (84), 1003 (M⁺) (100), 502 (83); HPLCretention time: 8.715 min., 47.17% (AUC); LRMS (ESI) m/z (relativeintensity): 1004 (M⁺+H) (71), 1003 (M⁺) (100), 986 (62), and astaxanthindisuccinate XV (18.61%).

EXAMPLE 30 Synthesis of Adenosine Monoester of Astaxanthin Disuccinate(LXIII)

To a solution of astaxanthin disuccinate (XV) (0.100 g, 0.125 mmol) inDCM/DMF (3 mL/3 mL) at room temperature was added DIPEA (0.653 mL, 3.75mmol), DMAP (0.3054 g, 2.50 mmol), HOBT-H₂O (0.1914 g, 1.25 mmol), and(−)-adenosine (0.3341 g, 1.25 mmol). The reaction mixture was stirred atroom temperature for 48 hours, at which time the reaction was dilutedwith DCM, quenched with brine/1M HCl (20 mL/3 mL), and then extractedwith DCM. The combined organic layers were concentrated to yieldadenosine monoester (LXIII) (21.13%) HPLC retention time: 9.005 min.,2.43% (AUC); LRMS (ESI) m/z (relative intensity): 1047 (M⁺+H) (36), 1046(M⁺) (57), 524 (100); HPLC retention time: 9.178 min., 10.92% (AUC);LRMS (ESI) m/z (relative intensity): 1047 (M⁺+H) (80), 1046 (M⁺) (100),829 (56), 524 (94); HPLC retention time: 9.930 min., 7.78% (AUC); LRMS(ESI) m/z (relative intensity): 1046 (M⁺) (100), 524 (34), andastaxanthin disuccinate XV (58.54%).

EXAMPLE 31 Synthesis of Maltose Diester of Astaxanthin Disuccinate(LXIV)

To a solution of astaxanthin disuccinate (XV) (0.100 g, 0.125 mmol) inDCM/DMF (3 mL/3 mL) at room temperature was added DIPEA (0.653 mL, 3.75mmol), DMAP (0.3054 g, 2.50 mmol), HOBT-H₂O (0.2297 g, 1.50 mmol), DIC(0.235 mL, 1.50 mmol), and (D)-maltose-H₂O (0.4504 g, 1.25 mmol). Thereaction mixture was stirred at room temperature for 36 hours, at whichtime the reaction was diluted with DCM, quenched with brine/1M HCl (20mL/3 mL), and then extracted with DCM. The combined organic layers wereconcentrated to yield maltose diester (LXIV) (25.22%) HPLC retentiontime: 7.411 min., 12.53% (AUC); LRMS (ESI) m/z (relative intensity):1468 (M⁺+Na) (18), 1067 (16), 827 (100); HPLC retention time: 7.506min., 12.69% (AUC); LRMS (ESI) m/z (relative intensity): 1468 (M⁺+Na)(52), 827 (76), 745 (100), and astaxanthin disuccinate XV (22.58%).

EXAMPLE 32 Synthesis of Resveratrol Esters of Astaxanthin Dissucinate(LXV, LXVI)

To a solution of astaxanthin disuccinate (XV) (0.100 g, 0.125 mmol) inDCM/DMF (3 mL/3 mL) at room temperature was added DIPEA (0.653 mL, 3.75mmol), DMAP (0.3054 g, 2.50 mmol), HOBT-H₂O (0.2297 g, 1.50 mmol), DIC(0.235 mL, 1.50 mmol), and resveratrol (0.2853 g, 1.25 mmol). Thereaction mixture was stirred at room temperature for 24 hours, at whichtime the reaction was diluted with DCM, quenched with brine/1M HCl (20mL/3 mL), and then extracted with DCM. The combined organic layers wereconcentrated to yield resveratrol monoester (LXV) (1.12%) HPLC retentiontime: 10.039 min., 1.12% (AUC); LRMS (ESI) m/z (relative intensity):1009 (M⁺+2H) (18), 1007 (M⁺) (21), 637 (100), resveratrol diester (LXVI)(60.72%) HPLC retention time: 10.324 min., 15.68% (AUC); LRMS (ESI) m/z(relative intensity): 1217 (M⁺) (28), 1007 (100), 609 (69), 504 (85);HPLC retention time: 10.487 min., 29.26% (AUC); LRMS (ESI) m/z (relativeintensity): 1218 (M⁺+H) (80), 1217 (M⁺) (100), 609 (60); HPLC retentiontime: 10.666 min., 15.78% (AUC); LRMS (ESI) m/z (relative intensity):1218 (M⁺+H) (84), 1217 (M⁺) (100), 609 (71), and no detectableastaxanthin disuccinate XV.

EXAMPLE 33 Synthesis of the Bis(2,3-di-OBz ascorbic acid)Bis(2-cyanoethyl) Phosphate Triester of Astaxanthin (LXVII)

To a stirring solution of astaxanthin 2E (100 mg, 0.168 mmol) in 2 mL ofdichioromethane were added triethylamine (63 μL, 0.454 mmol) and2-cyanoethyl diisopropylchlorophosphoramidite (79 μL, 0.353 mmol). Thereaction was stirred for 15 min then another portion of 2-cyanoethyldiisopropylchlorophosphoramidite (15 μL, 0.033 mmol) was added. After 1h reaction time, the solution was treated with 2,3-di-OBz ascorbic acid(149 mg, 0.386 mmol) and 1H-tetrazole (27 mg, 0.39 mmol). The reactionwas judged complete after 3 h by tlc analysis (40% EtOAc/heptane), andquenched by adding 30% hydrogen peroxide solution (48 mL, 0.42 mmol) in1 mL tetrahydrofuran. The reaction was diluted with 20 mL of DCM andwashed with 1 M sodium thiosulfate (20 mL), water (20 mL), and 0.25 MHCl (20 mL). The organic layer was dried over anhydrous sodium sulfate,filtered, and concentrated to give a crude red solid. Mass spectroscopyanalysis of the crude solid detected the mass ion of the desired product(+ESI, m/z 1595 (M⁺)).

EXAMPLE 34 Synthesis of Lycophyll Disuccinate (LXVIII)

To a stirring solution of lycophyll (28.0 mg, 0.0492 mmol) in 10 mL ofdichloromethane were added succinic anhydride (12.3 mg, 0.123 mmol) anddimethylaminopyridine (15.0 mg, 0.123 mmol). The reaction vessel waswrapped in aluminum foil and stirred at ambient temperature overnight.After 16 hours, the reaction was complete by TLC. The mixture was thenconcentrated to give a crude red solid. Mass spectroscopic analysis ofthe crude solid detected the mass ion of the desired product LXVIII(-APCI, m/z 767((M−H)⁻)). LC/MS analysis was performed on an Agilent1100 LC/MSD VL ESI system with Zorbax Eclipse XDB-C18 Rapid Resolution4.6×75 mm, 3.5 μM USUT002736; Temperature: 25° C.; Mobile Phase:(%A=0.025% TFA in H₂O; % B=0.025% TFA in MeCN), 70% A/30% B(start); holdat 30% B for 1 min, linear gradient to 98% B over 10 min, hold at 98% Bfor 9 min; Flow rate: 1.0 mL/min; Starting pressure: 112 bar; PDADetector 470 nm, 373 nm, 214 mn. LRMS: −mode, APCI.

EXAMPLE 35 Synthesis of Bis(methyl)phosphates of lutein (“xanthophyll”)(LXIX and LXX)

General. Reactions were performed under nitrogen (N₂) atmosphere andwere protected from direct light. Racemic lutein 2B (“xanthophyll”) waspurchased from ChemPacific. Flash chromatography was performed onNatland International Corporation 230-400 mesh silica gel using theindicated solvents. LC/MS was recorded on an Agilent 1100 LC/MSD VL ESIsystem; column: Zorbax Eclipse XDB-C18 Rapid Resolution (4.6×75 mm, 3.5μm, USUT002736); temperature: 25° C.; starting pressure: 107 bar; flowrate: 1.0 mL/min.; mobile phase (% A=0.025% TFA in H₂O, % B=0.025% TFAin acetonitrile) Method: 70% A/30% B (start), step gradient to 50% Bover 5 min., step gradient to 98% B over 8.30 min., hold at 98% B over25.20 min., step gradient to 30% B over 25.40 min.; PDA Detector: 470nm; LRMS: +mode, ESI.

Bis(methyl) phosphates of lutein (“xanthophyll”). To a solution oftrimethyl phosphite (0.533 mL, 4.52 mmol) in DCM (5 mL) at 0° C. wasadded I₂ (1.06 g, 4.20 mmol). The mixture was stirred at 0° C. for 10minutes or until all I₂ went into solution to produce a clear, colorlesssolution. The solution was allowed to warm to room temperature, and wasstirred for an additional 5 minutes. The solution was slowly addeddropwise to a mixture of xanthophyll (0.60 g, 1.05 mmol) and pyridine(3.40 mL, 42.0 mmol) at −78° C. The solution was stirred for 10 minutesat −78° C., quenched with brine, and extracted with DCM. The combinedorganic layers were washed with brine, dried over Na₂SO₄, andconcentrated. Purification of the residue using flash chromatography(66% hexanes/ethyl acetate, 1% TEA) yielded bis(methyl)monophosphateLXIX (0.080 g) HPLC retention time: 18.048 min.; LRMS (ESI) m/z(relative intensity): 676 (M⁺) (10), 675 (18), 659 (30), 533 (100); andbis(methyl) diphosphate LXX (0.130 g) HPLC retention time: 13.111 min.;LRMS (ESI) m/z (relative intensity): 807 (M⁺+Na) (15), 785 (M⁺+H) (10),676 (40), 675 (20), 659 (100), 533 (90).

Rigorous Determination of Water Solubility of the Disodium DisuccinateAstaxanthin Derivative (XVI)

A total of 30 mg of sample (disodium disuccinate astaxanthin derivative,as the all-trans mixture of stereoisomers 3S,3′S, meso, and 3R,3′R in a1:2:1 ratio) was added to 2 mL of sterile-filtered (0.2 μM Millipore®)deionized (DI) water in a 15 mL glass centrifuge tube. The tube waswrapped in aluminum foil and the mixture was shaken for 2 hours, thencentrifuged at 3500 rpm for 10 minutes. The aqueous solution wasfiltered through a 0.45 micron PVDF disposable filter device. A 1 mLvolume of filtrate was then diluted appropriately with DI water, and theconcentration of the solution was measured at 480 nm using a four pointcalibration curve prepared from fresh sample. After taking the dilutionsinto account, the concentration of the saturated solution of thedisodium disuccinate astaxanthin derivative was 8.64 mg/mL.

Experimental Data for Inhibition and/or Amelioration of DiseaseComparison of Radical-Cation Forming Ability: Non-esterified, FreeAstaxanthin and Diacid Disuccinate Astaxanthin Using Flash Photolysis

FIG. 27 and FIG. 28 depict the results of spectral analysis after flashphotolysis of the formation of triplet and carotenoid cation radicalstates for non-esterified, free astaxanthin 2E and the diaciddisuccinate astaxanthin derivative XV were obtained. Formation of thecarotenoid cation radical is a measure of the potential biophysicalbehavior of the novel derivative as an antioxidant. If a derivativeretains the antioxidant behavior of non-esterified, free astaxanthin,then all previously documented (i.e. literature precedent) therapeuticapplications for astaxanthin can be reasonably assumed for the novelderivative, including at least singlet oxygen quenching, lipidperoxidation chain-breaking, and/or direct radical scavenging.

Irradiating carotenoids (car) directly does not result in the formationof carotenoid triplets (³car); a photosensitizer is needed. In thisexperiment, nitronaftalin (NN) was used as the photosensitizer. Afterirradiation, the excited sensitizer (NN*) forms a sensitizer triplet(³NN). When ³NN encounters a carotenoid, energy and electron transferreactions with ³NN take place. The resulting relatively stable ³car andcarotenoid cation radicals (car⁺) are detected by characteristicabsorption bands. Non-polar solvents (e.g. hexane) favor the formationof ³car, and more polar solvents (alcohols, water) favor the formationof the car⁺. The anion radical of the sensitizer (NN) is not typicallyseen because of a low absorption coefficient.

A. Spectra with Astaxanthin Disuccinic Acid (astaCOOH).

Transient Absorption Spectra of AstaCOOH in Acetonitrile (MeCN),Sensitizer NN.

Negative peaks in the spectra demonstrate ground state depletion of NNand astaCOOH XV. The positive peak at 550 nm shows the formation of theastaCOOH XV triplet; the positive peak at 850 nm shows the formation ofthe astaCOOH XV cation radical. The ³car decays rather quickly. After 15μs, half of the ³car has disappeared, and after 50 μs, no ³car is left.The car⁺ is stable within this time frame.

B. Spectra with Reference Compound [Non-esterified, Free Astaxanthin(asta)].

Transient Absorption Spectra of Asta in Acetonitrile (MeCN), SensitizerNN.

The spectrum of asta 2E is nearly identical to that of astaCOOH XV.After 50 μs, the ³car has disappeared. During this time frame, the car⁺is stable. Negative and positive peaks in the absorption spectra forastaCOOH XV and asta 2E are superimposable.

Brief Discussion of Flash Photolysis Results:

There appears to be little difference between the diacid disuccinateastaxanthin derivative (astaCOOH, XV) and non-esterified, freeastaxanthin (asta, 2E) during flash photolysis experiments. AstaCOOH XVbehaves like asta 2E in the flash photolysis experiments. Therefore,esterification of free astaxanthin 2E with succinic acid does not alterthe photophysical properties and the cation radical lifetime. Bothcompounds were photostable during the flash photolysis experiments. Thedisuccinate astaxanthin XV derivative retains the potent antioxidantpotential of astaxanthin 2E, and is active in the esterified state. Itcan therefore be considered a “soft” drug (active as the modifiedentity)—and not a prodrug for therapeutic applications—conferring thevaluable propert(ies) of dual-phase radical scavenging activity to thisderivative (i.e. aqueous- and lipid-phase radical scavenging).

Induction of Connexin 43 Protein Expression

The methods for cell culture, Western blotting, quantitativedensitometric analysis, and total protein evaluation are described indetail in Rogers et al. (1990), with modifications suggested in Bertram(1999). In brief, mouse embryonic fibroblast CH3/10T^(1/2) cells weretreated with the following formulations in a 4 mL cell culture systemwith media containing 2% calf serum:

-   1. TNPB    [p-(E)-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-napthyl)propenyl    benzoic acid] 10⁻⁸ M in acetone [positive control for connexin 43    upregulation (4 μl in 4 mL]-   2. Disodium salt disuccinate astaxanthin derivative XVI/H₂0 at 10⁻⁵    M (40 μl in 4 mL)-   3. Disodium salt disuccinate astaxanthin derivative XVI/H₂0 at 10⁻⁶    M (4 μl in 4 mL)-   4. Disodium salt disuccinate astaxanthin derivative XVI/H₂0 at 10⁻⁷    M (1:10 dilution and 4 μl in 4 mL)-   5. Disodium salt disuccinate astaxanthin derivative XVI H₂O/ethanol    [EtOH] formulation at 10⁻⁵ M (40 μl in 4 mL)-   6. Disodium salt disuccinate astaxanthin derivative XVI H₂O/EtOH    formulation at 10⁻⁶ M (4 μl in 4 mL)-   7. Sterile H₂O control (40 μl in 4 mL)-   8. Sterile H₂O/EtOH control (20 μL EtOH, 20 μL H₂O in 4 mL)-   9. Media control (4 mL)

Cells were harvested after 96 hours incubation with test compounds andcontrol solutions. All media solutions were identical in color, howeverafter treatment with the disodium salt disuccinate astaxanthinderivative XVI at both 10⁻⁵ dilutions, the color subjectively changed toan orange-red color. Cells treated with TTNPB appeared striated withlight microscopy, evidence of differentiation to myocytes, an expectedresult in this cell culture system. After harvest and pelleting ofcells, tubes containing both 10⁻⁵ disodium salt disuccinate astaxanthinderivative XVI solutions were bright red; both 10⁻⁶ dilution tubes werea pink color. As documented previously for other colored carotenoids,this was subjective evidence for cellular uptake of the test compounds.

Cells were then lysed, and 50 μg of each protein was electrophoresed ona 10% polyacrylamide gel. The gel was then transferred to anitrocellulose filter. Total protein was assayed with Coomassie bluestaining (FIG. 29; lanes 6, 7, and 9 were smeared secondary to geltransfer, and were not included in the quantitative comparison [FIG.31)]. Western blotting was performed with anti-connexin 43 antibodiesfollowed by HRP chemiluminescence on a Biorad imager (FIG. 30). Theoriginal gel was stripped once, and the Western blot repeated twiceprior to visualization. The results were normalized to the Lane 8control (EtOH/H₂O), which demonstrates background constitutiveexpression of connexin 43 protein in a control condition (no testcompound). The results of relative connexin 43 induction by positivecontrols and test compounds are shown in FIG. 31.

Brief Discussion of Cx43 Results.

All disodium salt disuccinate astaxanthin derivative XVI formulationstested induced connexin 43 protein expression over the levels expressedconstitutively in water and ethanol/water controls (FIG. 31). Theprobability of detecting an induction of connexin 43 protein expressionin 5 separate test conditions in the absence of a true treatment effect(null hypothesis control μ₁=treatment mean μ₂) is 1 in 2⁵, or p=0.03.Disodium salt disuccinate astaxanthin derivatives XVI formulated inwater induced connexin 43 protein expression in each test condition(from 10⁻⁵ to 10⁻⁷ M). The decrease in the lowest disodium saltdisuccinate astaxanthin derivative XVI/water combination tested suggestsdose-dependency in the induced response. The relative induction wasincreased in the single test condition evaluated with a fmal ethanolicconcentration in media of 0.5%. This finding is highly suggestive ofincreased bioavailability of this formulation, as ethanol is known toreduce aggregation of disodium salt disuccinate astaxanthin derivativesXVI in aqueous solutions. Solutions of disodium salt disuccinateastaxanthin derivatives XVI in water at concentrations greater than 10⁻⁷and in ethanol/water combinations at 10⁻⁵ appear to have higherinductions levels than the positive TTNPB control. TTNPB is a highlypotent retinoid that is effective at inducing connexin 43 expression atthe 96-hour time point at 10⁻⁸ M.

Induction of Intercellular Gap Junctional Communication (GJC) in MurineFibroblasts by the Disodium Salt Disuccinate Astaxanthin Derivatives

A series of experiments were performed to assess the ability of thedisodium salt disuccinate astaxanthin derivatives XVI to induce gapjunctional communication (GJC) in an immortalized line of murinefibroblasts. Studies were conducted:

-   (1) at the functional level to measure cell/cell communication by    increased dye transfer between confluent cells in monolayer culture;-   (2) at the molecular level as measured by the ability of these    compounds to induce expression of connexin43 (Cx43) protein. Cx43 is    the structural unit of the intercellular channels in these    fibroblasts that allows GJC;-   (3) at the cellular level as shown by the ability of the disodium    salt disuccinate astaxanthin derivatives XVI to increase the number    and size of Cx43 immunoreactive plaques in regions of the plasma    membrane in direct contact with adjacent cells.-   (1) Communication Assays. Experiments were performed to assess the    ability of the disodium salt disuccinate astaxanthin derivative XVI    [as a statistical mixture of the all-trans (all-E) stereoisomers,    3S,3′S, meso, and 3R,3′R in 1:2:1 ratio] to enhance gap junctional    intercellular communication (GJC) between mouse embryonic fibroblast    C3H/10T 1/2 cells. This ability has been previously highly    correlated with the ability of carotenoids to inhibit    carcinogen-induced neoplastic transformation (Zhang, 1992).    Moreover, Cx43-mediated junctional communication between cardiac    myocytes is responsible for transfer of signals that maintain    synchronous contractions and prevent cardiac arrhythmias (Peters,    1995).

Junctional permeability was assayed by microinjection of the fluorescentdye Lucifer Yellow CH (Sigma, St. Louis, Mo.) into individual confluentcells essentially as described previously (Zhang, 1994). Briefly,confluent cultures of C3H/10T1/2 cells were treated for 4 days with: (1)the disodium salt disuccinate astaxanthin derivative XVI (1×10⁻⁵ M)dissolved in a 1:2 ethanol/water (EtOH/H₂O) formulation; (2) a syntheticretinoid, TTNPB (1×10⁻⁸ M) dissolved in tetrahydrofuran as a positivecontrol; or (3) 1:2 EtOH/H₂O treated cells as a negative control. Singlecells in each dish were identified under phase contrast optics andpressure injected using a microinjection needle (Eppendorf, Hamburg,Germany) loaded with the fluorescent dye Lucifer Yellow as a 10%solution. The needle was controlled by a remote micromanipulator andcells and microscope were positioned on a pneumatic anti-vibrationtable. Successful injection of Lucifer Yellow was confirmed by briefillumination with UV light, which causes yellow fluorescence of LuciferYellow. This dye is sufficiently small to pass through gap junctions andis electrically charged, and can thus only enter cells adjacent to theinjected cell if they are in junctional communication. After 2 minutesto allow for junctional transfer, digital images were taken under UVillumination. The number of fluorescent cells adjacent to the injectedcell was later determined by digital image analysis using an unbiaseddensity threshold method and the SigmaScan software program (JandelScientific). This number of communicating cells was used as an index ofjunctional communication, as described previously (Hossain, 1993).

The results of this analysis demonstrated that the disodium saltdisuccinate astaxanthin derivative XVI (1×10⁻⁵ M) dissolved in a 1:2EtOH/H₂O formulation effectively increased the extent of junctionalcommunication over that seen in 1:2 EtOH/H₂O treated controls. Of 22microinjected treated cells 15 (56%) were functionally coupled by gapjunctions, in contrast to only 3 out of 11 (27%) control cells. Thesedifferences were statistically different (p<0.04; paired Student'st-test). Representative photomicrographs are shown in FIG. 14:

Panel A: treatment with the statistical mixture of stereoisomers of thedisodium salt disuccinate astaxanthin at 1×10⁻⁵ M in 1:2 EtOH/H₂O;

Panel C: 1:2 EtOH/H₂O solvent negative control;

Panel E: TTNPB at 1×10⁻⁸ M in tetrahydrofuran as solvent, positivecontrol; and

Panels B, D, F: digital analysis of micrographs A, C, E respectively,demonstrating pixels above a set threshold positive for Lucifer Yellowfluorescence. Because cell nuclei have the most volume, they accumulatethe most Lucifer Yellow and exhibit the most fluorescence.

-   (2) Molecular studies. Both the mixture of stereoisomers of the    disodium salt disuccinate astaxanthin derivative XVI and purified    enantiomeric forms of the disodium salt disuccinate astaxanthin    derivative XVI (3S,3′S, meso, and 3R,3′R forms at >90% purity by    HPLC) increase expression of Cx43 protein in murine fibroblasts as    assessed by immuno—(Western) blotting essentially as described    (Zhang, 1992 and 1994). Briefly, mouse embryonic fibroblast    C3H/10T1/2 cells were cultured in Eagle's basal medium with Earle's    salts (Atlanta Biologicals, Atlanta, Ga.), supplemented with 5%    fetal calf serum (Atlanta Biologicals, Atlanta, Ga.) and 25, μg/mL    gentamicin sulfate (Sigma, St. Louis, Mo.), and incubated at 37° C.    in 5% CO₂. On the 7^(th) day after seeding in 100 millimeter (mm)    dishes, the confluent cells were treated for four days with the    disodium salt disuccinate astaxanthin derivatives XVI and then    harvested and analyzed for Cx43 protein induction as described.    Protein content was measured using the Protein Assay Reagent kit    (Pierce Chemical Co., Rockford, Ill.) according to manufacturer's    instructions. Cell lysates containing 100 μg of protein were    analyzed by Western blotting using the NuPage western blotting kit    and apparatus (Invitrogen, Carlsbad, Calif.) and Cx43 protein    detected using a rabbit polyclonal antibody (Zymed, San Francisco,    Calif.) raised against a synthetic polypeptide corresponding to the    C-terminal domain of mouse, human and rat Cx43. Cx43 immunoreactive    bands were visualized by chemiluminescence using an anti-rabbit    HRP-conjugated secondary antibody (Pierce Chemical Co., Rockford,    Ill.). Digital images were obtained with a cooled CCD camera, and    quantitative densitometry was then performed (Bio-Rad, Richmond,    Calif.). Equal protein loading of the lanes was confirmed by    staining with Coomassie blue protein stain and digital image    analysis.

In this experiment the disodium salt disuccinate astaxanthin derivativesXVI were added to cell cultures in a formulation of 1:2 ethanol/H₂O at1×10⁻⁵ M. The statistical mixture of stereoisomers and purifiedenantiomeric forms demonstrated increased expression of Cx43 incomparison to cell cultures treated with 1:2 ethanol/H₂O alone (FIG. 15Aand FIG. 15B). Treatment with the statistical mixture of stereoisomersof the disodium salt disuccinate astaxanthin derivative XVI elicited thehighest induction level of Cx43 of all derivatives tested. Theseinduction levels were several-fold less than induction levels seen withthe retinoids tetrahydrotetramethylnapthyl propenylbenzoic acid (TTNPB)(Hoffman-LaRoche, Nutley, N.J.) and retinyl acetate (Sigma, St. Louis,Mo.) included as positive controls; this relative potency difference isconsistent with previous studies.

-   (3) Cellular studies. The statistical mixture of stereoisomers of    the disodium salt disuccinate astaxanthin derivative XVI increases    assembly of Cx43 in treated murine 10T1/2 cells in regions of    cell/cell contact consistent with formation of functional gap    junctions.

In this experiment expression and assembly of Cx43 into plaques wasassessed by immunofluorescent staining. Procedures were essentially asdescribed in Zhang (1992). Briefly, confluent cultures of C3H/10T1/2cells were grown on Permanox plastic 4-chamber slides (Nalge NuncInternational, Naperville, Ill.) and treated for 4 days with: (1) thedisodium salt disuccinate astaxanthin derivative XVI (statisticalmixture of stereoisomers) dissolved in a 1:2 EtOH/H₂O formulation; (2)the retinoid TTNPB at 1×10⁻⁸ M in tetrahydrofuran as a positive control;or (3) 1:2 EtOH/H₂O as a solvent control. Cells were fixed with −20° C.methanol overnight, washed in buffer, blocked in 1% bovine serum albumin(Sigma, St. Louis, Mo.) in PBS, and incubated with the rabbit polyclonalanti-Cx43 antibody (Zymed, San Francisco, Calif.) as in (2) above anddetected with Alexa568 conjugated anti-rabbit secondary (MolecularProbes, Eugene, Oreg.). Slides were illuminated with 568 nm light andimages were acquired at a wavelength of 600 nm using the Zeiss Axioscopelight microscope and a Roper Scientific cooled CCD camera. Slidestreated with the TTNPB retinoid control and the statistical mixture ofstereoisomers of the disodium salt disuccinate astaxanthin derivativeXVI at 1×10⁻⁵ M exhibited assembly of immunoreactive Cx43 into plaquesin regions of the cell membrane in direct contact with adjacent cells.Such assembly is consistent with the location and formation of plaquesof gap junctions, known to be formed by the aggregation of multipleindividual gap junctions in cell populations which are junctionallyconnected (Perkins, 1997). In cultures treated with solvent as control,such immunoreactive plaques were infrequent and were smaller than thosedetected in cells treated with the statistical mixture of stereoisomersof the disodium salt disuccinate astaxanthin derivative XVI or withTTNPB as positive control. The frequency of these plaques and their sizeis consistent with the functional differences in gap junctionpermeability as detected by the Lucifer Yellow dye transfer experimentsdescribed in section 1, and FIG. 14 (TTNPB>statistical mixture ofstereoisomers of the disodium salt disuccinate astaxanthin XVI>solventcontrol), and with the degree of induction of Cx43 as detected in theimmunoblot experiments described in section 2 and FIG. 15.

Representative photomicrographs are shown in FIG. 16.

Inhibition of Carcinogen-Induced Neoplastic Transformation byNon-Esterified, Free Astaxanthin 2E in Murine Fibroblasts

Non-esterified, free astaxanthin 2E is generated in the mammalian gutafter oral administration of esterified astaxanthin. Only freeastaxanthin is found in mammalian plasma and solid organs. This wasagain demonstrated in single- and multiple dose oral pharmacokineticstudies; the results are described herein. Inherent esterase activity ofserum albumin, and the action of promiscuous esterases in serum andsolid organs rapidly generates non-esterified, free astaxanthin afterparenteral administration of the disodium disuccinate astaxanthinderivative (XVI). Flash photolysis experiments also demonstrated thatthe disodium disuccinate astaxanthin derivative XVI and non-esterified,free astaxanthin have identical antioxidant behavior in terms offormation of the carotenoid cation radical. An experiment was performedto assess the ability of non-esterified, free astaxanthin (the in vivofinal cleavage product of the disodium salt disuccinate astaxanthinderivative (XVI), tested as the all-trans mixture of stereoisomers3S,3′S, meso, and 3R,3′R in a 1:2:1 ratio) to inhibit neoplastictransformation in the C3H10T1/2 cell culture model developed in the labof the late Charles Heidelberger (Reznikoff, 1973). This cell culturesystem has been shown to effectively mimic the initiation andtransformation events of tumor formation in whole animals (Bertram,1985). In these cells, treatment with the carcinogenic polycyclichydrocarbon 3-methylcholanthrene (MCA) produces an initiation event in asmall proportion of treated cells that leads 5 weeks later tomorphological transformation in these cells, exhibited by the presenceof transformed foci. Injection of these transformed cells into syngeneicmice results in the formation of sarcomas at the site of injectiondemonstrating the carcinogenic nature of the transformation (Reznikoff,1973). This assay has been adapted to the detection of cancer preventiveagents (Bertram, 1989), and cancer preventive retinoids and carotenoidshave been demonstrated to inhibit transformation in this system(Bertram, 1991; Pung, 1988; and Merriman, 1979).

This experiment was conducted according to protocols establishedpreviously (Bertram, 1991 and Pung, 1988). In brief, the 10T1/2 cells,derived from mouse embryonic fibroblasts, were seeded at a density of10³ cells/60 mm dish in Eagle's Basal Media (BME) (Atlanta Biologicals,Atlanta, Ga.), supplemented with 4% fetal calf serum (AtlantaBiologicals, Atlanta, Ga.) and 25 μg/mL gentamicin sulfate (Sigma, St.Louis, Mo.). Cells were treated 24 hours later with 5.0 μg/ml MCA(Sigma, St. Louis, Mo.) in acetone or with 0.5% acetone (finalconcentration) as a control. Media was changed 24 hours after MCAtreatment. Cells were treated with astaxanthin in THF or with retinolacetate in acetone 7 days later, and re-treated every 7 days for 4weeks. Other dishes were treated with the appropriate solvent controls.After 5 weeks from the start of the experiment, cells were fixed withmethanol and stained with 10% Giemsa stain (Sigma, St. Louis, Mo.) andscored for type II and type III foci as per Reznikoff (1973).

The results of this analysis demonstrated that 4-week treatment withastaxanthin 2E caused a concentration-dependent decrease in the numbersof MCA-induced transformed foci in comparison to cells treated with MCAand with THF as a solvent control (depicted in FIG. 34). FIG. 34 depictseffects of non-esterified, free astaxanthin (as the all-trans mixture ofstereoisomers) on MCA-induced neoplastic transformation. Graphrepresents a total of 68 cultures treated with astaxanthin 2E at 3×10⁻⁶M, 1×10⁻⁶ M and 3×10⁻⁷ M, delivered in a THF vehicle of 0.3%, 0.1% and0.03%, respectively. Controls were as follows: a total of 16 dishes didnot receive carcinogen and were treated with 0.05% ethanol solvent;controls did not exhibit any transformation events. A total of 20 disheswere treated with MCA and 1% THF solvent, yielding a transformation rateof 0.92 foci/dish. Percent reduction (% reduction) of transformation inastaxanthin-treated dishes was calculated by a comparison of the meanfoci/dish of each treatment with the MCA-treated controls. Inferentialstatistics were performed using the paired Student's t-test; calculatedP values of 0.00004, 0.00001, and 0.00006, respectively, were obtained.P<0.05 was considered significant. Treatment with 3×10⁻⁶ M astaxanthin2E resulted in complete suppression of the transformed phenotype (FIG.35). FIG. 35 depicts a comparison of astaxanthin-treated dish to controldishes. Representative dishes treated with: A, no MCA with solventcontrol; B, MCA 5.0 μg/ml with 1% THF as solvent control; C, MCA with3×10⁻⁶ M astaxanthin (as the all-trans mixture of stereoisomers) in THF.It is notable that this level of inhibition far exceeded that reportedpreviously for all other carotenoids tested using identical protocols(Bertram, 1991). A comparison of the current data to data previouslyreported for percent reduction in neoplastic transformation at theconcentrations tested revealed astaxanthin 2E to be a far more potentinhibitor of transformation than either β-carotene or canthaxanthin(FIG. 36). FIG. 36 depicts a comparison of astaxanthin 2E (as themixture of stereoisomers) to previously tested carotenoids. Data wascompiled comparing the percent reduction of MCA-induced neoplasticallytransformed foci/dish in cultures treated with astaxanthin 2E to thepercent reduction of foci/dish from data previously reported by theBertram laboratory after treatment with β-carotene and canthaxanthin(Bertram, 1991) using identical protocols. The percent reduction at thehighest concentration tested previously (1×10⁻⁵ M) is reported here forβ-carotene and canthaxanthin; this higher concentration of astaxanthin2E was not utilized because of astaxanthin's greater measured activityat lower concentrations. These studies demonstrate the potential for thecleaved astaxanthin moiety of the synthesized derivative to be a highlyeffective cancer chemoprevention agent, after both oral and parenteraladministration. Coupled with the liver accumulation pharmacokinetic dataalso reported here (after both single- and multiple-dose strategies),the use of this compound forms a particularly useful embodiment.

Inhibition of Reactive Oxygen Species

In an experiment, neutrophils were isolated on a Percoll gradient fromwhole blood from a human volunteer. The isolated neutrophils were thenre-suspended in phosphate-buffered saline, and maximally stimulated withphorbol ester to induce the respiratory burst and production ofsuperoxide anion. To the solution of activated human neutrophils, thedisodium salt disuccinate astaxanthin derivative XVI was added atvarious concentrations, and the superoxide signal [as measured withelectron paramagnetic resonance (EPR) spectroscopy] was subsequentlymeasured. The disodium salt disuccinate astaxanthin derivative XVI (asthe mixture of stereoisomers) reduced the measured superoxide anionsignal in a dose-dependent manner (FIG. 2); near complete suppression ofthe superoxide anion signal was achieved at 3 mM concentration. FIG. 2demonstrates the strong superoxide signal after activation in controls,then the results of titration with the disodium salt disuccinateastaxanthin derivative XVI from 100 μM to 3 mM. The disodium saltdisuccinate astaxanthin derivative XVI tested at 100 μM scavenged 28% ofthe total signal. At 3 mM, almost no superoxide signal remained. Theseresults demonstrate that cardioprotection in ischemia-reperfusioninjury, as has been demonstrated with the other anti-neutrophilinterventions described above, can also be achieved with the carotenoidderivative described here. In addition to reducing the superoxide anionsignal important in ischemia-reperfusion injury, it is also likely thatmyocardial salvage can be achieved with the described carotenoidderivative, as superoxide anion plays a major role in tissue injury anddeath during prolonged myocardial ischemia.

FIG. 3 depicts an effect of a disodium salt disuccinate astaxanthinderivative XVI/Vitamin C solution on reactive oxygen species (superoxideanion) as monitored using EPR spectroscopy. The solution included amixture of about 2 to about 1 of vitamin C to disodium salt disuccinateastaxanthin derivative XVI respectively. The disodium salt disuccinateastaxanthin derivative XVI/Vitamin C solution reduced the measuredsuperoxide anion signal in a dose-dependent manner (FIG. 3); completesuppression of the superoxide anion signal was achieved at 0.02 μMconcentration. FIG. 3 demonstrates the strong superoxide signal afteractivation in controls, then the results of titration with the disodiumsalt disuccinate astaxanthin derivative XVI/Vitamin C solution from 0.01μM to 0.02 μM.

In a third experiment, neutrophils were again isolated on a Percollgradient from whole blood from a second human volunteer. The isolatedneutrophils were then re-suspended in phosphate-buffered saline, andmaximally stimulated with phorbol ester to induce the respiratory burstand production of superoxide anion. To the solution of activated humanneutrophils, the hydrochloride salt dilysinate astaxanthin derivative(XX) was added at four (4) concentrations, and the superoxide signal (asmeasured with EPR spectroscopy) was subsequently measured. Thehydrochloride salt dilysinate astaxanthin derivative XX also reduced themeasured superoxide anion signal in a dose-dependent manner (FIG. 21),from approximately 5% reduction at 1 μM to 98% reduction at 3 mM. Onceagain, near complete suppression of the superoxide anion signal wasachieved at 3 mM concentration. This carotenoid derivative XX showedscavenging efficacy at low concentration (1 μM), as well as the abilityfor increased concentrations of the derivative in this in vitro assay tonearly completely eliminate the superoxide anion signal. The activity ofderivative XX in vitro as an aqueous scavenger again suggests that thederivatives (disodium disuccinate astaxanthin XVI, hydrochloride saltdilysine astaxanthin XX) will act as soft drugs (i.e. active as theintact, uncleaved novel derivatives) and not pro-drugs (inactive untilcleavage to free astaxanthin) in vivo. The aqueous solubility of thisderivative (XX) was greater than 50 mg/mL, demonstrating the utility ofthe methods of the present invention to increase the water solubility ofthe parent carotenoids (in this case astaxanthin), from nearly zeroinherent water solubility to the high mg/mL range.

Direct Superoxide Anion Scavenging by a Disoditim DisuccinateAstaxanthin Derivative XVI: Relative Efficacy of IndividualStereoisomers versus the Statistical Mixture of Stereoisomers byElectron Paramagnetic Resonance Imaging

Materials

Non-esterified, all-E astaxanthin 2E [1:2:1 statistical mixture ofstereoisomers 3S,3′S, meso (identical 3S,3′R and 3′S,3R), and 3R,3′R]was purchased from Buckton Scott (India) and used as supplied (>95%purity by HPLC). Astaxanthin 2E was dissolved in HPLC gradedimethylsulfoxide (DMSO; Sigma-Aldrich, St. Louis, Mo.). The disodiumdisuccinate derivatives XVI of astaxanthin 2E were tested separately innine formulations: statistical mixture of stereoisomers (as forastaxanthin, above, a 1:2:1 mixture of all-E; labeled as “mixture” inall tables and figures); 3S,3′S, and 3R,3′R (optical isomers orenantiomers); and meso (mixture of identical 3S,3′R and 3′S,3R;diastereomers of the enantiomeric pair). All disuccinate derivativeswere synthesized at >90% purity by HPLC. The disuccinate derivativeswere first tested at the appropriate final concentrations in pureaqueous solution (deionized water) from stock solutions of 10 mM. Eachof the four disuccinate derivatives were then tested from stocksolutions prepared in a 1:2 mixture of ethanol (final concentration ofEtOH in stock solution 33 ⅓%; final concentration in isolated neutrophilassay 0.3%; HPLC grade ethanol, Sigma-Aldrich, St. Louis, Mo.) at 10 mM.The 3S,3′S derivative was also tested from a 50% EtOH concentrationstock solution (final concentration in isolated neutrophil assay 0.5%).Ethanolic formulation of the disuccinate derivatives has been shown tocompletely disaggregate the supramolecular assemblies which form in pureaqueous solution, providing monomeric solutions of the derivativesimmediately before introduction into the test assay. Ethanol alonenegative controls (0.3% and 0.5% fmal EtOH concentrations in isolatedneutrophil assay) and superoxide dismutase mimetic positive control (10μM final concentration; Metaphore® Pharmaceuticals, Inc., St. Louis,Mo.) were also performed.

A carotenoid derivative [Succinic acidmono-(4-{18-[4-(3-carboxy-propionyloxy)-2,6,6-trimethyl-3-oxo-cyclohex-1-enyl]-3,7,12,16-tetramethyl-octadeca-1,3,5,7,9,11,13,15,17-nonaenyl}-3,5,5-trimethyl-2-oxo-cyclohex-3-enyl)ester;FIG. 17] and its stereoisomeric forms were synthesized, disodiumdisuccinate derivatives XVI of astaxanthin 2E, in all-trans (all-E)form. The derivatives are symmetric chiral molecules with 2 chiralcenters at the 3 and 3′ carbon positions, comprising 4 stereoisomers:3R,3′R and 3S,3′S (optical isomers, or enantiomers), as well as thediastereomeric meso forms (identical 3R,3′S and 3′R,3S). The statisticalmixture of stereoisomers synthesized from the commercial source ofastaxanthin contains 3R,3′R, meso (identical 3R,3′S and 3′R,3S), and3S,3′S stereoisomeric forms in a 1:2:1 ratio. All individualstereoisomers and the statistical mixture were synthesized at >90%purity by HPLC, allowing direct comparison of the individual efficacy ofthese forms as direct radical scavengers. The all-E forms of thestereoisomers used in this study were linear, rigid molecules(bolaamphiphiles) owing to the lack of cis (or Z) configuration(s) inthe polyene chain of the spacer material.

The disodium disuccinate diesters XVI of astaxanthin 2E demonstrateincreased water “dispersibility” over the parent compound astaxanthin2E. The water dispersibilities of the individual stereoisomers and thestatistical mixture were all greater than 8 mg/mL (approximately 10 mM),allowing them to be introduced into the buffered aqueous test systemwithout a co-solvent. The tendency for the parent carotenoids such asastaxanthin 2E (Salares, 1977), as well as carotenoid derivatives (e.g.capsanthin derivatives) (Zsila, 2001 and Bikadi, 2002) to formsupramolecular assemblies in aqueous solution was also observed for thederivatives tested in the current study. Supramolecular self-assemblyresults in aggregates of significant size in aqueous solution, andprevents maximum direct interaction of aggregated molecules with radicalspecies. Therefore, a comparison of the direct scavenging behavior ofthe novel astaxanthin derivatives was conducted in both pure aqueousformulation as well as with the co-solvent ethanol. In stock solutions,a 1:2 concentration of EtOH/water was shown to completely disaggregatethe statistical mixture, meso, and 3R,3′R derivatives; a 50% ethanolicstock solution was required to completely disaggregate the 3S,3′Sisomer. The scavenging ability of the compounds was also tested relativeto negative (i.e. ethanol vehicle) and positive [superoxide dismutase(SOD) mimetic, free racemic astaxanthin in DMSO] controls.

Leukocyte Purification and Preparation

Human polymorphonuclear leukocytes (PMNs) were isolated from freshlysampled venous blood of a single volunteer (S.F.L.) by Percoll densitygradient centrifugation, which yielded PMNs with a purity of >95%. Each10 mL of whole blood was mixed with 0.8 mL of 0.1 M EDTA and 25 mL ofsaline. The diluted blood was layered over 9 mL of Percoll at a specificdensity of 1.080 g/mL. After centrifugation at 400×g for 20 min at 20°C., the plasma, mononuclear cell, and Percoll layers were removed.Erythrocytes were lysed by addition of 18 mL of ice-cold water for 30 s,followed by 2 mL of 10× PIPES buffer (25 mM PIPES, 110 mM NaCl, and 5 mMKCl, titrated to pH 7.4 with NaOH). Cells were pelleted at 4° C., thesupernatant was decanted, and the procedure was repeated. After thesecond hypotonic lysis, cells were washed twice with PAG buffer (PIPESbuffer containing 0.003% human serum albumin and 0.1% glucose).Afterward, PMNs were counted by light microscopy on a hemocytometer. Thefinal pellet was then suspended in PAG-CM buffer (PAG buffer with 1 mMCaCl₂ and 1 mM MgCl₂).

EPR Measurements

All EPR measurements were performed using a Bruker ER 300 EPRspectrometer operating at X-band with a TM₁₁₀ cavity. The microwavefrequency was measured with a Model 575 microwave counter (EIPMicrowave, Inc., San Jose, Calif.). To measure O₂ generation fromphorbol-ester (PMA)-stimulated PMNs, EPR spin-trapping studies wereperformed using DEPMPO (Oxis, Portland, Oreg.) at 10 mM. 1×10⁶ PMNs werestimulated with PMA (1 ng/mL) and loaded into capillary tubes for EPRmeasurements. To determine the radical scavenging ability ofnonesterified, free “racemic” astaxanthin in DMSO and the disodium saltdisuccinate derivatives XVI in each of the nine formulations, PMN's werepre-incubated for 5 minutes with compound followed by PMA stimulation aspreviously described. The instrument settings used in the spin-trappingexperiments were as follows: modulation amplitude, 0.32 G; timeconstant, 0.16 s; scan time, 60 s; modulation frequency, 100 kHz;microwave power, 20 milliwatts; and microwave frequency, 9.76 GHz. Thesamples were placed in a quartz EPR flat cell, and spectra wererecorded. The component signals in the spectra were identified andquantified as reported (Lee, 2000).

Statistical Analysis

Statistical analyses were performed with the NCSS statistical softwarepackage (NCSS 2001 and PASS 2002, Kaysville, Utah). All statisticaltests were performed at an α=0.05.

Brief Discussion of EPR Results:

The potent SOD mimetic produced by Metaphore, Inc. served as a positivecontrol at study outset. As has been observed repeatedly in the Zweierlaboratory, the 10 μM dose in water-only vehicle nearly completelyeliminated the superoxide anion signal as detected with DEPMPO (97%inhibition; Table 1). An ethanol-alone negative control (finalconcentration 0.3%) was also evaluated, as ethanol shows minorscavenging activity in these systems; 5.7% inhibition was seen at thisconcentration. This amount of inhibition was not subtracted fromformulations containing ethanol in the descriptive data in Table 1, asthe utility of the dosing vehicle itself (disodium disuccinatederivative XVI in EtOH) in direct scavenging was being evaluated in thisstudy. Non-esterified, free astaxanthin in DMSO (100 μM) was evaluatedas a reference standard for direct comparison to the novel derivativessynthesized for this study; mean inhibition of the astaxanthin/DMSOvehicle was 28% (Table 1).

FIG. 18 shows the relative scavenging ability of each of the 3stereoisomers (mixture and 3 individual stereoisomers) in water, at afmal concentration of 100 μM. Except for the 3R,3′R enantiomer (28.7%inhibition), all other derivative formulations showed decreasedscavenging ability relative to the astaxanthin/DMSO formulation (range−2.0% to 19.3% inhibition; Table 1). As can be seen, the 3S,3′Sformulation did not exhibit any mean scavenging activity. Whenintroduced into the isolated neutrophil test system in ethanolicformulation, however, in each case the scavenging ability increased overthat of the same derivative formulated in water (FIG. 19; range 38.0% to42.5%). It is important to note that the 3S,3′S derivative wasformulated in 50% EtOH for this comparison. A trend toward increasedscavenging capacity over astaxanthin in DMSO was seen for the novelderivatives in ethanolic formulation, but after subtraction of the meanscavenging ability of the ethanol vehicle (final concentration in thetest assay 0.3%), the trend was not significant (NS). In addition, nosignificant differences in mean scavenging ability were observed amongthe 4 formulations of novel derivatives tested in ethanol (FIG. 19).

FIG. 20 shows the results of titration of superoxide signal inhibitionby increasing concentrations of the mixture of stereoisomers of disodiumdisuccinate astaxanthin XVI in ethanolic formulation. As theconcentration was increased from 100 μM to 3 mM, near completeinhibition of superoxide signal was noted (95.0% inhibition at the 3 mMdose; Table 1 and FIG. 18). The dose-response curve was non-linear.Adjusting for percent inhibition and tested dose, the disodiumdisuccinate derivative was between one and two orders of magnitude lesspotent than the SOD mimetic used as a positive control in the currentstudy (Table 1). Table 1 depicts descriptive statistics for variousformulations of disodium disuccinate derivatives of astaxanthin testedin the current study. Sample sizes of 3 or greater were evaluated foreach formulation, with the exception of 3S, 3′S in 50% EtOH stocksolution (N=2), and SOD mimetic (positive control, N=1) evaluated atstudy outset.

TABLE 1 Mean (% Sample Solvent Concentration N inhibition) S.D. SEM MinMax Range Astaxanthin DMSO 0.1 mM 4 28.0 7.6 3.8 20 35 15 2E MixtureWater 0.1 mM 3 19.3 0.6 0.3 19 20 1 Mixture EtOH 0.1 mM 3 38.0 8.7 5.032 48 16 Mixture EtOH 0.5 mM 3 60.1 7.2 4.2 56 69 13 Mixture EtOH 1.0 mM3 78.0 8.2 4.7 71 87 16 Mixture EtOH 3.0 mM 3 95.0 4.9 2.8 89 98 9 MesoWater 0.1 mM 3 15.7 5.9 3.4 9 20 11 Meso EtOH 0.1 mM 4 42.5 3.4 1.7 3846 8 3R, 3′R Water 0.1 mM 3 28.7 15.0 8.7 13 43 30 3R, 3′R EtOH 0.1 mM 540.8 7.5 3.3 30 50 20 3S, 3′S Water 0.1 mM 3 −2.0 4.4 2.5 −7 1 8 3S, 3′SEtOH 0.1 mM 6 21.3 4.9 2.0 15 29 14 3S, 3′S EtOH 0.1 mM 2 38 1.4 1.0 3739 2 (50%) Control Water 0.0 mM 10 0.0 ND ND ND ND ND Control EtOH 0.3%final 3 5.7 2.5 1.5 3 8 5 SOD Water  10 μM 1 97.0 ND ND ND ND ND mimeticBrief Discussion of EPR Results.

Astaxanthin 2E is a potent lipophilic antioxidant that normally exertsits antioxidant properties in lipid-rich cellular membranes,lipoproteins, and other tissues (Britton, 1995). Derivatives ofastaxanthin—with increased utility as water-dispersible agents—have theability to directly scavenge aqueous-phase superoxide anion produced byisolated human neutrophils after stimulation of the respiratory burst.

The pure aqueous formulations of the novel derivatives were less potentthan the ethanolic formulations in terms of direct scavenging ability.Supramolecular assembly of the water soluble carotenoid derivatives insome solvents (e.g., water) may explain their lack of potency in thosesolvents. The aggregation is of the helical, “card-pack” type, withaggregates greater than 240 nm in size forming in pure aqueous solution.Increasing 15 ionic strength of buffer solutions may increase both thesize and stabilility of these aggregates. The radical scavenging abilityof these aggregates will be diminished over the monomeric solutions ofthe same compounds; in fact, no scavenging ability was seen for the3S,3′S stereoisomer dissolved in water (Table 1, FIG. 18). Care must betaken in preparation of formulations for in vitro and in vivo testing,as supramolecular assembly limits the number of molecules available forinteraction with radical species. The size of the aggregates must alsobe taken into account, as aggregates containing as many as 10⁶ moleculesand reaching 300 nm or greater in size have been described (Bikadi,2002).

Titration of the disodium disuccinate astaxanthin derivative XVI dose to3 mM (as the mixture of stereoisomers in 1:2 EtOH/water) demonstratednear complete suppression of the superoxide anion signal (95%inhibition), as measured with the DEPMPO spin trap (FIG. 20). Thedose-response curve was non-linear, requiring increasing doses fornear-complete suppression of radical signal (FIG. 20). At the lowestconcentration tested (100 μM), nearly 40% of the signal was inhibited.The potency of the disodium disuccinate astaxanthin derivative at thisdose can be compared directly with the superoxide dismutase (SOD)mimetic used as a positive control in the current study (97% inhibitionat 10 μM). The results show that as an aqueous-phase radical scavenger,the disodium disuccinate astaxanthin derivative XVI is one to two ordersof magnitude less potent than the SOD mimetic. However, in vivo, thesederivatives decay to free astaxanthin, which becomes active in thelipid-rich membranes of cells [including the mitochondrial and nuclearmembranes (Goto, 2001)], therefore providing dual protection (aqueousand lipid-phase radical scavenging), not achievable with water-solubleproteins and enzyme mimetics. Non-esterified, free astaxanthin (whenprovided as a dietary supplement at 0.02% of feed wt/wt) iscardioprotective against the ROS-mediated strenuous exercise insult toboth skeletal and cardiac muscle (Aoi et al. 2003). Therefore, thischaracteristic (i.e. dual-phase radical scavenging) should provideadditional utility for this class of compounds as clinical therapeuticagents in those indications for which radical and reactive oxygenspecies prevention is important (Cross, 1987).

The study demonstrates for the first time direct scavenging ofsuperoxide anion detected by EPR spectroscopy by a group of carotenoidderivatives. The compounds were found to form supramolecular assembliesin pure aqueous solution. Formation of supramolecular assemblies maylimit their scavenging potency relative to monomeric solutions of thesame compounds. No significant differences in scavenging ability wereseen among the 3 stereoisomers of the carotenoid derivatives.Dose-ranging studies revealed that the concentration of derivative couldbe increased to near-complete suppression of the induced superoxideanion signal. As potential in vivo therapeutic agents, this class ofcompounds may be used as both an aqueous phase and lipid phasescavenger, which should find wide application in those acute and chronicdisease conditions for which potent radical scavengers have demonstratedefficacy.

Direct Superoxide Anion Scavenging by the Disodium DisuccinateDi-Vitamin C Astaxanthin Derivative

In an electron paramagnetic resonance (EPR) spectroscopy experiment,neutrophils were isolated on a Percoll gradient from whole blood from ahuman volunteer. The isolated neutrophils were then re-suspended inphosphate-buffered saline, and maximally stimulated with phorbol esterto induce the respiratory burst and production of superoxide anion. Tothe solution of activated human neutrophils, the disodium disuccinatedi-vitamin C astaxanthin derivative (XXIII) (semi-systematic nameSuccinic acid4-[18-(4-{3-[2-(3,4-dihydroxy-5-oxo-2,5-dihydrofuran-2-yl)-2-hydroxy-ethoxycarbonyl]-propionyloxy}-2,6,6-trimethyl-2-oxo-cyclohex-1-enyl)-3,7,12,16-tetramethyl-octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]-3,5,5-trimethyl-2-oxo-cyclohex-3-enylester2-(3,4-dihydroxy-5-oxo-2,5-dihydroftiran-2-yl)-2-hydroxy-ethylester) was added at various concentrations, and the superoxide signal(as measured with EPR spectroscopy) was subsequently measured. Thedisodium disuccinate di-vitamin C astaxanthin derivative (XXII) reducedthe measured superoxide anion signal in a dose-dependent manner (FIG.33); complete suppression of the superoxide anion signal was achieved at60 μM concentration. This represents a 50-fold increase in potency overthe disodium disuccinate astaxanthin derivative (XVI) also synthesizedfor the current series of experiments. The purity of the derivative astested was 88% (by HPLC area under the curve, or AUC). The carotenoidderivative—designed to be a “soft-drug” by esterification to the 6-OHposition of each vitamin C—preserved the antioxidant function of theindividual vitamin C molecules. The potency of the derivative (XXIII)approached that of the formulation of disodium disuccinate astaxanthin(XVI) with free vitamin C in a 1:2 molar ratio (which completelysuppressed the superoxide anion signal in a 20 μM/40 μM disodiumdisuccinate astaxanthin derivative (XVI)/free vitamin C formulation).Derivative (XXIII), which generates 2 moles of free vitamin C and 1 moleof non-esterified, free astaxanthin for every mole of derivative in vivomay be particularly preferred for certain clinical indications.Derivative (XXIII) will also likely show increased efficacy in thoseclinical situations in which aqueous-phase scavenging (by the intactparent derivative, as well as free vitamin C) as well as lipid-phasescavenging (by non-esterified, free astaxanthin) are important forreduction in the pathology attributable to ROS and other radical speciesinjury.

Infarct Size Reduction in Male Sprague-Dawley Rats

FIG. 4, FIG. 25, and FIG. 26 depict graphical representations of thereduction of infarct size in male Sprague-Dawley rats. MaleSprague-Dawley rats were pre-treated with the disodium salt disuccinateastaxanthin derivative XVI (as the mixture of stereoisomers) in aqueoussolution before performing an occlusion and inducing a myocardialinfarction. Male Sprague-Dawley rats (175-200 grams) were anaesthetizedwith 100 mg/kg of Inactin, instrumented, and the heart exposed. The leftcoronary artery had a suture placed around it and was subjected to 30minutes of total coronary artery occlusion followed by 2 hours ofreperfusion, at which time infarct size was measured in hearts excisedfrom the animal. The hearts were washed in buffer and incubated intriphenyltetrazolium chloride (TTC) staining solution kept at 37° C. inphosphate buffer at pH of 7.40. Infarct size (IS) was expressed as a %of the area at risk (IS/AAR, %). Systemic blood pressure, heart rate,blood gases and body temperature were monitored throughout theexperiment, and temperature and blood gases were tightly controlled atnormal physiological levels. 25, 50, or 75 mg/kg of the disodium saltdisuccinate astaxanthin derivative XVI or sterile saline vehicle wasadministered I.V. by tail vein injection every day for 4 days prior tothe infarct experiment on day 5 and subsequent infarct sizedetermination.

Brief Description of Salvage Results.

Infarct size reduction, and the corresponding myocardial salvage,increased linearly, and significantly, with dose (P=0.001**). At themaximum dose tested, 75 mg/kg, mean myocardial salvage was 56%, whichapproaches that achievable with ischemic pre-conditioning strategies.Volume limitations for single-dose I.V. injection in this rat precludedtesting of higher doses; however, the significant linear correlation(P<0.001**; r²=0.67) between non-esterified, free plasma levels ofastaxanthin 2E and IS/AAR, % suggested that at doses of approximately120 to 125 mg/kg, 100% salvage might be achieved. This is the firstdemonstration of cardioprotection by a carotenoid derivative.

Pharmacokinetics, Increased Bioavailability, and Increased Target TissueDistribution of the Orally Administered Disodium Disuccinate AstaxanthinDerivative

Plasma Pharmacokinetics

Single dose oral pharmacokinetic parameters (including C_(max), T_(max),AUC₍₀₋₇₂₎V_(d), and clearance) of the disodium disuccinate astaxanthinderivative XVI were determined in male C57BL/6 mice. The animals wereadministered the derivative orally at a single maximum dose (500 mg/kg)shown in prior studies to likely be efficacious in preventing the injurysecondary to CCl₄-administration in Sprague-Dawley rats (100 mg/kg bodyweight in those studies). Samples for HPLC analysis of levels of freeastaxanthin in plasma and liver were obtained at the following timepoints, from at least 3 animals per time point:

Time 0 [immediately before dosing of test compound], 2, 4, 6, 8, 12, 16,24, 48, and 72 hours after ingestion.

Additional samples, with N<3, were taken at other intervals (10, 14, and36 hours; Tables 2 and 3). Non-esterified, free astaxanthin levels weredetermined in this study as carotenoid esters are completely cleaved inthe mammalian gut to free carotenoid, which moves passively across theenterocyte.

Brief Description of Experimental Methods: Plasma Pharmacokinetics

Male C57BL/6 mice, approximately 25 g, were housed in cages (threemice/cage) and fed standard mouse chow (Purina Mouse Chow, RalstonPurina, St. Louis) and water ad libitum for at least five days prior tothe start of the experiment. The disodium disuccinate astaxanthinderivative XVI was mixed with the following components to make anemulsion suitable for oral gavage:

-   -   Sterile filtered (0.2 micron Millipore®) water;    -   Olive oil (Bertolli USA, Inc., Secaucus, N.J.);    -   Soybean lecithin, Type IV-S (Sigma-Aldrich Co., St. Louis, Mo.;        catalog number P3644).

The disodium disuccinate astaxanthin derivative XVI demonstrateswater-solubility of approximately 8.64 mg/mL in pure aqueousformulation. In the emulsion described above, solubility was increasedto approximately 50 mg/mL, allowing for dosing up to 500 mg/kg by gavagein these animals. This significant 6-fold increase in solubility in thedosing vehicle greatly facilitated gavage studies in these small mice.

Methods for preparing the emulsion were as follows:

-   -   (1) Add 80 mg of soy lecithin (Sigma catalog P3644) to 5.0 mL        water. Vortex intermittently for approximately 30 minutes in a        15 mL centrifuge tube until the suspension is uniform;    -   (2) Add 2.5 mL olive oil at room temperature and vortex. This        produces a uniform, thick, cloudy yellow suspension. This        emulsion material may be stored either at room temperature or in        the refrigerator at 4° C. If stored, vortex immediately before        adding the disodium disuccinate derivative XVI in 3 (below);    -   (3) Add the disodium disuccinate astaxanthin derivative XVI at        50 mg/mL directly to the emulsion. The compound readily enters        into a uniform suspension at this concentration. Vortex        immediately prior to gavage to assure uniform suspension; and    -   (4) The material has the potential to clog the mouse gavage        needle. Rinse the gavage needle after every 2 gavages.

The emulsion was given by oral gavage at 500 mg/kg body weight in asingle dose. Food was withdrawn from all cages the evening prior to theexperiment. One hour after administration of the emulsion, food andwater were restored to all animals.

The methods for whole blood and tissue sampling, sample extraction, andHPLC analysis have been described in detail (Osterlie, 2000). Briefly,whole blood was collected in EDTA-containing Vacutainer® tubes, andplasma subsequently prepared by centrifugation at 4° C., 1500×g for 20minutes. Plasma samples were then aliquoted and snap frozen in liquidnitrogen prior to transport and HPLC analysis.

Tissue Accumulation

Free astaxanthin concentration was also determined, at the same timepoints as for plasma samples, in liver. Livers were removed from eachanimal in the pharmacokinetic study after sacrifice, and snap frozen inliquid nitrogen. Liver tissue was prepared for HPLC analysis asdescribed (Jewell, 1999). Therefore, simultaneous examination of liveraccumulation of free astaxanthin was performed at the same time pointsas the plasma analyses.

Brief Description of Experimental Methods: Liver Accumulation of FreeAstaxanthin

Up to 300 mg of liver from each animal was snap frozen in liquidnitrogen. Tissue homogenization and extraction were performed with amixture of chloroform/methanol/water, according to the methods of Jewell(1999). Non-esterified, free astaxanthin accumulation in liver was thenevaluated by HPLC as described above for plasma samples.

Brief Discussion of Pharmacokinetic Results

Summary tables of plasma and liver levels of free astaxanthin at theappropriate sampling interval(s) are shown as Tables 2 and 3. Plasma andliver non-esterified free astaxanthin areas under the curve vs. time(AUC's) are also included in Tables 2 and 3. The results demonstratethat for each sampling interval, the levels of free astaxanthin in liverare equal or greater to that in plasma. This improved tissue-specificdelivery to the liver is unprecedented in the literature; in fact, liverlevels of free astaxanthin are typically lower than the correspondinglevels in plasma at equivalent time points post-dose (Kurihara, 2002).Thus, the disodium disuccinate astaxanthin derivative XVI in theemulsion described above is a superior vehicle for delivery oftherapeutic concentrations of free carotenoid to tissues of interestafter oral dosing.

TABLE 2 Plasma Levels of Non-Esterified, Free Astaxanthin Time Sampleasta nM asta mg/kg mean mg/kg S.D. 0 PK01 0.00 0.00 PK03 0.00 0.00 PK060.00 0.00 PK15 0.00 0.00 PK16 0.00 0.00 PK20 0.00 0 2 PK10 38.04 0.02PK12 0.00 0.00 PK21 0 0 PK22 0 0 PK27 0 0 PK34 0 0 PK42 311.73 0.19 PK4374.08 0.04 PK48 48.41 0.03 PK59 318.83 0.19 0.05 0.077 4 PK07 46.18 0.03PK11 115.63 0.07 PK14 20.97 0.01 PK17 40.57 0.02 PK23 214.95 0.13 PK24179.33 0.11 PK28 PK44 80.48 0.05 PK45 67.16 0.04 PK57 119.02 0.07 PK58147.85 0.09 0.062 0.039 6 PK13 40.57 0.02 PK18 605.01 0.36 PK25 262.730.16 PK26 377.14 0.22 PK32 PK46 739.91 0.44 PK60 167.39 0.1 PK61 131.740.08 0.197 0.154 8 PK36 PK47 435.17 0.26 PK49 371.11 0.22 PK62 148.980.09 PK68 405 0.24 PK69 306.86 0.18 PK70 29.98 0.02 0.168 0.094 10 PK3112 PK37 PK63 37.19 0.02 PK64 10.93 0.01 PK67 8.12 0 PK71 53.19 0.03 PK727.66 0 PK73 8.46 0.01 0.012 0.012 14 PK51 0 0 PK52 3.14 0 0 0 16 PK658.44 0.01 PK66 10.47 0.01 PK75 28.24 0.02 PK76 4.51 0 0.010 0.008 24PK29 0 0 PK35 18.03 0.01 PK39 13.93 0.01 PK50 1.51 0 PK53 0 0 0.0040.005 36 PK38 21.37 0.01 0.01 48 PK30 0 0 PK33 0 0 PK54 22.71 0.01 PK550 0 0.003 0.005 72 PK40 1.7 0 PK41 PK56 0 0 PK74 1.92 0 0 0

TABLE 3 Liver Levels of Non-Esterified, Free Astaxanthin Time Sampleasta nM asta mg/kg mean mg/kg S.D. 0 PK01 0.00 0.00 PK03 0.00 0.00 PK060.00 0.00 PK15 0.00 0.00 PK16 7.67 0.00 PK20 8.18 0.00 0.00 0 2 PK10139.37 0.08 PK12 30.66 0.02 PK21 414.34 0.25 PK22 725.87 0.43 PK27294.07 0.18 PK34 165.32 0.1 PK42 689.36 0.41 PK43 129.66 0.08 PK48 244.50.15 PK59 564.28 0.34 0.20 0.146 4 PK07 103.07 0.06 PK11 243.4 0.15 PK1489.18 0.05 PK17 1565.15 0.93 PK19 1373.34 0.82 PK23 2558.63 1.52 PK244701.95 2.8 PK28 1023.78 0.61 PK44 359.73 0.21 PK45 211.35 0.13 PK57322.06 0.19 PK58 500.82 0.3 0.648 0.812 6 PK13 374.28 0.22 PK18 2970.441.77 PK25 3515.52 2.1 PK26 2087.8 1.24 PK32 687.99 0.41 PK46 1070.130.64 PK60 974.69 0.58 PK61 841.37 0.5 0.933 0.690 8 PK36 1290.15 0.77PK47 230.88 0.14 PK49 1115.86 0.67 PK62 1247 0.74 PK68 1263.31 0.75 PK691036.29 0.62 PK70 1518.27 0.9 0.637 0.244 10 PK31 1303.06 0.78 0.780 12PK37 3225.35 1.92 PK63 921.74 0.55 PK64 713.97 0.43 PK67 410.93 0.24PK71 1382.45 0.82 PK72 567.95 0.34 PK73 716.89 0.43 0.468 0.579 14 PK51141.9 0.08 PK52 179.51 0.09 0.085 0.007 16 PK65 240.6 0.14 PK66 340.380.2 PK75 788.66 0.47 PK76 499.84 0.3 0.278 0.144 24 PK29 440.72 0.26PK35 321.14 0.19 PK39 155.42 0.09 PK50 156.61 0.09 PK53 89.18 0.05 0.1360.086 36 PK38 658.41 0.39 0.39 48 PK30 106.07 0.06 PK33 116.79 0.07 PK5417.81 0.01 PK55 28.79 0.02 0.04 0.029 72 PK40 33.52 0.02 PK41 11.66 0.01PK56 9.21 0.01 PK74 19.31 0.01 0.013 0.005

Pre-treatment (15 days to 6 weeks) is often required when carotenoidssuch as provided in oral vehicle or in feed to achieve efficaciouslevels in liver-injury studies (Kang, 2001; Kim, 1997; Aoi et al. 1993).In this case, therapeutic levels (200 nM or above) were achieved with asingle dose.

The C_(max) (Table 4) of 0.9 mg/L is also unprecedented in rodents,animals which absorb only a small percentage of the oral dose ofcarotenoids. It is significant that these plasma and liver levels offree carotenoid were obtained after just a single dose of compound inthe emulsion vehicle. In humans, Osterlie et al. (2000) have describedC_(max) plasma levels of 1.3 mg/L after a single dose of 100 mg(approximately 1.1 mg/kg oral dose) of non-esterified, free astaxanthinin olive oil vehicle. Humans typically absorb 40 to 50% of the oral doseof carotenoid when provided in fatty vehicle, as opposed to a fewpercentage points for rodents. Therefore, the current study demonstratesachievement of nearly 70% of the C_(max) in humans with the emulsionvehicle developed for rodents, greatly increasing the utility of thisderivative for hepato-protection studies.

TABLE 4 pK Parameters Parameter Liver Plasma *C_(max) (mg/L) 0.9 0.2**T_(max) (hr) 6 6 Elimination half-life (hr) 11.655 3.938 Eliminationrate (1/hr) 0.059 0.176 ***AUC₍₀₋₇₂₎ (mg hr/L) 15.8 1.2 ***AUC∞ (mghr/L) 15.9 1.2 Oral clearance (L/hr) 15.856 216.822 Volume ofdistribution (L/kg) 263.9 1232.1 *Maximal concentration **Time atmaximum concentration ***Area under the curve

Reduction of Experimental Infarct Size and Circulating Levels ofC-reactive Protein in Rabbits after Parenteral Administration of Cardax™(Disodium Disuccinate Astaxanthin Derivative):

The influence of parenteral administration of the disodium disuccinateastaxanthin derivative (XVI) on induced infarct size and induced levelsof circulating C-reactive protein (CRP) in rabbits was investigatedusing the methods of Barrett et al. (2002) with slight modifications.The purpose of the current study was to investigate the ability of thedisodium disuccinate astaxanthin derivative (XVI) to reduce inflammationas measured by CRP in the setting of experimental myocardialischemia-reperfusion injury in the rabbit heart. It has been suggestedthat CRP, commonly used as a marker for the acute inflammatory(“acute-phase”) response, may actually have a pro-inflammatory effectmediated through the activation of the complement cascade. Myocardialischemia-reperfusion injury, which is accompanied by an increase in theformation of oxygen radicals (ROS), has also been shown to activate thecomplement system. It has been demonstrated that (1) the endogenousincrease in plasma CRP secondary to a remote inflammatory lesion wasassociated with an increase in myocardial tissue injury secondary toregional ischemia and reperfusion; (2) this increase in injury(manifested as increased infarct size) was mediated by complementactivity; and (3) CRP was an “effector”, and not merely an indirectmeasure of systemic inflammation, in this system. Therefore, reductionof circulating CRP levels, together with the reduction(s) in infarctsize previously noted with Cardaxm™ in rodents, would form a powerfulanti-inflammatory therapeutic modality in the acute coronary syndromesetting.

In brief, male New Zealand white rabbits (2.25-2.5 kg) were used for thestudy. The acute phase inflammatory response was induced by subcutaneousinjection of four aliquots (0.5 mL each) of 1% croton oil in corn oilbeginning on the second day of pre-treatment with Cardax™. EitherCardax™ (at 50 mg/kg IV by ear vein injection) in water or equal volumesof sterile saline were given once per day for 4 days prior toexperimental infarction on day 5. The time course of increases incirculating CRP levels were obtained as described previously (Barret etal. 2002), using an ELISA-based method with anti-rabbit CRP antibodies.On the final day of the experiment (day 5: approximately 24 hours afterthe last drug infusion), the rabbits were anesthetized with a mixture ofxylazine (3 mg/kg) and ketamine (35 mg/kg) followed by pentobarbital (90mg/kg) intramuscularly. Additional pentobarbital was administered asnecessary to maintain anesthesia. After tracheotomy, the rabbits wereventilated with room air, and the heart was exposed via a leftthoracotomy. The heart was then supported in a pericardial cradle and a3-0 silk ligature was placed around the left anterior descendingcoronary artery. The artery was occluded for 30 minutes by exertingtraction on the ligature and subsequently reperfused for 180 minutes.Shortly before completing the protocol, a venous blood sample wasobtained for determination of plasma CRP.

At the completion of the reperfusion phase of the protocol, the heartswere removed and cannulated by the aorta on the Langendorff perfusionapparatus. The hearts were then perfused with a modified Krebs-Henseleitbuffer for 10 to 15 minutes (20-25 mL/minute). At the conclusion of thisperiod, the hearts were perfused with 80 mL of 0.4%2,3,5-triphenyltetrazolium chloride (TTC) at 37° C. for determination ofthe area-at-risk (AAR). The left circumflex coronary artery was thenligated in the same area as it was during the surgicalpreparation/experimental infarction. At this time, the perfusion pumpwas stopped, and 3.0 mL of Evan's blue dye was injected slowly into thehearts through a sidearm port connected to the aortic cannula. Thesolution was allowed to distribute through the heart for approximately30 seconds. The hearts were then cut into six transverse sections atright angles to the vertical axis. The right ventricle, apex, and atrialtissue were discarded. Tissue demarcated by a purple/blue colorrepresented the region perfused by the noninfarct-related coronaryartery distribution. Both surfaces of each transverse section weretraced onto clear acetate sheets that were scanned and subsequentlydigitized to calculate infarct area. Total area at risk was expressed asa percentage of the left ventricle. Infarct size was then expressed as apercentage of area at risk.

Mean infarct size in control animals and Cardax™-treated animals isshown in FIG. 37. Levels of circulating CRP in control animals andCardax™-treated animals (shown as the mean difference between baselinelevels and induced levels at the time of reperfusion) is shown in FIG.38. Reductions in infarct size of approximately 55.4% percent were seenin Cardax™-treated rabbits; ischemic area-at-risk was similar in bothgroups. Similarly, the mean increase in circulating CRP levels incontrols (+23.5%) over baseline was completely abrogated in theCardax™-treated animals, to mean levels below those observed at baseline(−15.7%). As CRP is both an effector in the acute coronarysyndrome—resulting in an increased infarct size in the presence ofelevated levels of this acute phase reactant—and a strong independentpredictor of cardiovascular risk in primary and secondary preventioncardiac patients—reductions in the levels of this circulating proteinforms a strong therapeutic modality.

Oral Administration of Disodium Disuccinate Astaxanthin Reduces AlanineAminotransferase (ALT) Elevations Produced by Lipopolysaccharide (LPS)in Mice:

The following study evaluates the utility of oral administration of thedisodium disuccinate astaxanthin derivative XVI for hepatoprotectiveeffects in a model of LPS-induced liver injury in mice.

Brief Description of Experimental Methods:

Three-month old male ICR mice were treated with LPS and galactosamine inorder to induce liver injury (Leist, 1995). Mice were first orallygavaged with either an olive oil/water/lecithin emulsion (10 mL/kg, or0.3 mL for a 30 gram mouse), or the same emulsion containing thedisodium disuccinate astaxanthin derivative XVI (50 mg/mL) for a finaldisodium disuccinate astaxanthin dose of 500 mg/kg. Two hours later micewere injected intraperitoneally (IP) with either saline (10 mL/kg) or asolution of E. coli LPS (3 mg/kg, Sigma catalog number L-3755) andD-galactosamine (700 mg/kg). Animals were sacrificed by carbon dioxide(CO₂) asphyxiation 5 hours after the IP injection, and plasma was thencollected for ALT determination.

Brief Description of LPS-Induced Injury Results.

These initial results demonstrated that the disodium disuccinateastaxanthin derivative had no effect on plasma ALT in the salineinjected (liver-injury sham-treated control) animals. In control animalsgavaged with emulsion only (without the derivative), there was a greaterthan 3-fold increase in ALT. In animals that received the emulsion withdisodium disuccinate astaxanthin derivative XVI at 500 mg/kg included,the ALT elevation was substantially reduced (N=3 animals per group),demonstrating the efficacy of the compound in reducing ALT, a serummarker of hepatocyte necrosis in these animals. As LPS-induced liverinjury is mediated by ROS (including the radical nitric oxide NO.), andsubstantial systemic inflammation occurs after LPS insult, for whichnon-esterified, free astaxanthin is protective (Ohgami et al. 2003), theutility of the novel derivative for clinical indications in which suchinflammation is promoted represents a particularly useful embodiment.

Accumulation of Free Astaxanthin in Plasma and Liver after Multiple DoseOral Administration in Black Mice

In this pharmacokinetic study, with methods as described herein, eleven(11) individual daily oral doses of the disodium disuccinate astaxanthinderivative XVI (500 mg/kg) were given by oral gavage in the emulsionvehicle to black mice, and the accumulation of free astaxanthin inplasma and liver was measured in three (3) animals at the probableC_(max) and T_(max) (6 hours). Probable C_(max) and T_(max) (6 hours)was deduced from plasma and liver samples in the prior single dose oralpharmacokinetic study. Accumulation of non-esterified, free astaxanthinin plasma and liver after single emulsion doses was assessed. The meanplasma concentration for all animals tested was 381 nM. Mean liverconcentration for all animals tested was 1735 nM. In the single dosestudy, on average, a protective level (set at the antioxidant ED₅₀ fornon-esterified, free astaxanthin of 200 nM) was achieved in both plasmaand liver; the mean liver concentration achieved was almost 9 times theprotective level.

In the multiple dose study, both peak and trough levels were taken (peaklevels taken 6 hours after dosing at the probable C_(max); trough levelsobtained 6 hours after C_(max), or 12 hours post-dose). Mean peak levelsin plasma at peak and trough, respectively, were 485 nM and 231 nM; meanpeak levels in liver at peak and trough, respectively, were 1760 nM and519 nM. Again, in each case protective levels were achieved andmaintained to 11 days post-multiple dosing; in the case of liver, levelsalmost 9 times the protective level were achieved. Again, at each timepoint after multiple dosing, the accumulation in liver was greater thanthat observed in plasma, demonstrating the increased utility of thisdosing vehicle for targeting to this solid organ (FIG. 32). It is alsoapparent from this data set that chronic administration of the disodiumdisuccinate astaxanthin derivative XVI will be efficacious inhepatoprotection.

Accumulation of Free Astaxanthin in Myocardium (Heart) and Brain afterSingle Dose Oral Administration in Black Mice

A single maximum dose of the disodium disuccinate astaxanthin derivativeXVI (500 mg/kg) was given by oral gavage in the emulsion vehicle toblack mice, and the accumulation of non-esterified, free astaxanthin wasmeasured in four (4) animals at the probable C_(max) and T_(max) (6hours), as deduced from plasma and liver samples in the prior study.Accumulation of non-esterified, free astaxanthin in heart after a singledose (mean +/−SEM of 4 animals=693.25 +/−272 nM) paralleled that seenwith accumulation of non-esterified, free astaxanthin in liver. At eachtime point, the accumulation in heart was greater than that observed inplasma, demonstrating the increased utility of this dosing vehicle fortargeting to solid organs. Accumulation of non-esterified, freeastaxanthin in the CNS (brain) was less striking (mean +/−SEM of 4animals=3.6+/−1.7 nM), suggesting that penetration of the blood-brainbarrier (BBB) was possible, but that chronic, multiple-doseadministration may be necessary to achieve protective levels for thoseCNS applications (Alzheimer's disease, stroke, etc.).

Interaction of the Disodium Salt Disuccinate Derivative ofmeso-Astaxanthin with Human Serum Albumin (HSA)

Poor aqueous solubility of most carotene carotenoids, and the vastmajority of xanthophylls limits their use as aqueous-phase singletoxygen quenchers and radical scavengers. Chemical modifications whichincrease the apparent solubility and/or dispersibility of thecarotenoids have found application in basic science as well as clinicalresearch. However, the tendency for the parent carotenoids and novelderivatives to form supramolecular assemblies in aqueous solutionwarrants comprehensive evaluation of such behavior prior to moving intoin vitro and in vivo assays of the efficacy of such compounds.

FIG. 5 depicts a carotenoid derivative, the disodium salt disuccinatederivative XVI (dAST) of synthetic meso-astaxanthin(3R,3′S-dihydroxy-β,β-carotene-4,4′-dione), in all-trans (all-E) form.The symmetric C₄₀-xanthophyll used to generate the new derivative hastwo chiral centers at the 3 and 3′ positions. In aqueous solutionC₄₀-xanthophyll exhibits no optical activity, as these stereocentershave opposite absolute configurations and internally compensate eachother. Natural carotenoid molecules possessing carboxylic functionalitybind preferentially to human serum albumin (HSA), the most abundantprotein in the blood. Since albumin binding strongly influences thepotential in vivo biochemical activities of a given compound, circulardichroism (CD), ultraviolet-visible (UV/Vis) and fluorescencespectroscopy were used to characterize the interaction of this novelcarotenoid derivative with fatty acid-free HSA. The protein binding andaggregation properties were investigated of this symmetric carotenoidattached through direct esterification to a moiety with carboxylate endgroups, forming a rigid, long-chain, highly unsaturated dianionicbolamphiphile. It was verified that in buffer solution in the absence ofprotein, the meso-carotenoid formed closely-packed H-type (card-pack)aggregates exhibiting no CD Cotton effects (CE). At low ligand/protein(L/P) molar ratios, however, the meso-carotenoid immediately andpreferentially associated with HSA in monomeric fashion, suggesting thatthe secondary chemical interactions (van der Waals forces, hydrogenbonding) that permit supramolecular assembly in aqueous solution wereovercome in a biologically relevant environment. Above 1:1ligand/protein molar ratio the meso-carotenoid molecules again began toaggregate; the aggregation observed at these ratios was chiral,resulting in a supramolecular structure showing intense, exciton-type CDactivity.

Brief Description of Experimental Methods

The novel derivative dAST XVI was synthesized from crystallineastaxanthin 2E [3R,3′R,3R,3 ′S,3S,3′S (25:50:25)], a statistical mixtureof stereioisomers obtained commercially (Buckton Scott, India). Theastaxanthin stereoisomers were separated by high-pressure liquidchromatography (HPLC), allowing for the synthesis of the purifiedmeso-disodium salt disuccinate derivative XVI for testing in the currentstudy. The all-trans (all-E) form of the meso stereoisomer used was alinear, rigid molecule owing to the lack of cis (or Z) configuration(s)in the polyene chain of the spacer material (FIG. 5). The disodium saltdisuccinate derivative XVI of synthetic meso-astaxanthin wassuccessfully synthesized at >99% purity by HPLC.

Materials

Essentially fatty acid-free human serum albumin (catalog No. A-1887, lotNo. 14H9319) were obtained from Sigma and used as supplied.Double-distilled water and spectroscopy grade dimethyl sulfoxide (DMSO,Scharlau Chemie S. A., Barcelona, Spain) and ethanol (Chemolab,Budapest, Hungary) were used. All other chemicals were of analyticalgrade.

Preparation of Stock Solution of dAST XVI

After dissolution of the meso-carotenoid in DMSO, 100 μl of DMSOsolution was added to 2 mL ethanol in a rectangular cuvette with 1 cmpathlength. The absorption spectrum was registered between 260 and 650nm. Concentration was calculated from the light absorption value at theλ_(max) (ε_(478 nm)=116,570 M⁻¹ cm⁻¹).

Preparation of HSA Solutions

For spectroscopic sample preparation, HSA was dissolved in pH 7.4 Ringeror 0.1 M pH 7.4 phosphate buffer solutions. Albumin concentration wascalculated with the value of E_(1 cm) ^(1%)=5.31, using experimentallyobtained absorbance data at 279 nm. The molecular weight of HSA wasdefined as 66500 Da.

Circular Dichroism and UV/Vis Absorption Spectroscopy

CD and UV spectra were recorded on a Jasco J-715 spectropolarimeter at25±0.2 and 37±0.2° C. in a rectangular cuvette with 1 cm pathlength.Temperature control was provided by a Peltier thermostat equipped withmagnetic stirring. All spectra were accumulated three times with abandwidth of 1.0 nm and a resolution of 0.5 mn at a scan speed of 100nm/min. Induced CD was defined as the CD of the DAST XVI-HSA mixtureminus the CD of HSA alone at the same wavelengths, and is expressed asellipticity in millidegrees (mdeg).

CD/UV/Vis Titration of HSA with dAST XVI in pH 7.4 Ringer and 0.1 MPhosphate Buffer Solutions at 37° C.

Ringer buffer, L/P values from 0.007 to 0.10:2 mL of 1.6×10⁻⁴ M HSAsolution was placed in the cuvette with 1 cm optical pathlength andsmall amounts of the ligand stock solution (c=2.2×10⁻⁴) were added withan automatic pipette in 10 μL aliquots. Ringer buffer, L/P values from0.82 to 13.13:2 ml of 2.3×10⁻⁶ M HSA solution was placed in the cuvettewith 1 cm optical pathlength and μL volumes of the ligand stock solution(c=3.9×10⁻⁴) were added with an automatic pipette. Phosphate buffer, L/Pvalues from 0.82 to 13.10:2 mL of 2.2×10⁻⁶ M HSA solution was placed inthe cuvette with 1 cm optical pathlength and μL volumes of the ligandstock solution (c=3.6×10⁻⁴) were added with an automatic pipette.

Measurement of the Intrinsic Fluorescence of HSA in the Presence of dASTXVI

2 mL of 4.2×10⁻⁶ M HSA solution was prepared in a 1 cm rectangular cellin 0.1 M pH 7.4 phosphate buffer. 1.3×10⁻⁴ and 3.3×10⁻⁴ Mmeso-carotenoid DMSO solutions were consecutively added in μL volumes tothe cuvette in the sample chamber of the Jasco J-715 spectropolarimeter.The resulting sample solution was excited between 240 and 360 nm in 0.5nm wavelength increments. Total fluorescence intensity was collected ateach wavelength with a Hamamatsu H5784-type photomultiplier detectormounted on a right angle to the light source. In the sample solution,initial and final concentrations of HSA and DAST were 4.2×10⁻⁶M-4.0×10⁻⁶ M and 1.3×10⁻⁷ M-1.4×10⁻⁵ M, respectively. Themeso-carotenoid/HSA molar ratio was varied between 0.03 and 3.53. Duringthe fluorescence measurements, final DMSO concentration did not exceed 5v/v %. A control experiment was also performed, in which thefluorescence of HSA during addition of 20, 50 and 100 μL DMSO to thesolution was measured.

Brief Discussion of UV/Vis and CD Spectroscopy Results

UV/Vis and CD Spectral Properties of dAST XVI in Ethanol and AqueousBuffer Solution

Because of its extended π-system, DAST XVI exhibited intense lightabsorption in the visible spectrum (FIG. 6). The main bell-shapedabsorption band centered at 481.5 nm was due to the lowest energyelectronic dipole allowed, a π→π* transition polarized along the longaxis of the polyene chain. At room temperature, lack of fine structureis typical for carotenoids containing one or more conjugated carbonylgroups. However, the vibrational sub-bands were indeed present beneaththis curve, as revealed by the second derivative of the spectrum (FIG.6). Additionally, in the near-UV region, further transitions werepresent. According to theoretical calculations performed on polyenemodels, the electronic transition moment (μ) of the moderately intenseband around 300 nm is polarized parallel to the long axis of the dASTXVI molecule. At the same time, the band at 371 nm μ is oriented alongthe twofold, C₂ symmetry axis of the conjugated system. The weak n→π*transitions of the carbonyl groups were obscured by the other bands. Asexpected, the meso-carotenoid compound did not show any CD bands inethanol since the effects of the two opposite chiral centers (3R,3′S)canceled each other (data not shown).

In Ringer buffer solution, the principal absorption band of dAST XVIchanged, exhibiting a large blue-shift (2541.6 cm⁻¹) as well asbandwidth narrowing (FIG. 7). These spectral changes indicated theformation of so-called “card-pack” aggregates, in which the moleculeswere held together in close proximity (within a few angstroms) by bothexclusion from the aqueous environment and H-bonding interactions. As aresult, the excited-state wave functions of the polyene chains weredelocalized inter-molecularly, allowing exciton resonance interaction tooccur between neighboring molecules. This interaction resulted in ahigh-energy exciton peak in the UV/Vis spectrum. Due to unfavorablesteric interactions arising among the bulky end-groups, parallelalignment of the polyene chains is not allowed; the long axes of theseparate molecules instead close a definite intermolecular overlayangle. In such cases, carotenoid aggregates built up by chiral monomersalso exhibit induced Cotton effects (CE) due to the chiralintermolecular arrangement determined by asymmetric centers. Incontrast, the meso-carotenoid compound demonstrated no optical activityin the aggregated state in solution (data not shown) due to the lack ofnet chirality of the molecules.

Optical Properties of ST XVI in the Presence of Human Serum Albumin atLow Ligand/protein Molar Ratios

Upon addition of dAST to the HSA solution prepared in pH 7.4 Ringerbuffer, two definite, oppositely-signed induced CD bands appearedbetween 300 and 450 nm with a zero cross-over point at 367 nm (FIG. 8).The figure inserts show the intensities of the induced Cotton effectsand the main absorption band at different VP ratios (Δε and ε values arecalculated with respect to the total meso-carotenoid concentration).Magnitudes of the CEs increased with increasing concentration of theligand, however, their shape and wavelength positions remain unchanged.As mentioned above, there are two transitions below 450 nm which mightbe responsible for the observed optical activity. The absorption bandaround 300 nm has transition symmetry B, and the corresponding electricand magnetic transition moments are perpendicular to the twofoldsymmetry axis along the polyene chain. The electric and magnetictransition moments of the band at 372.5 nm are polarized parallel to theC₂ axis, its transition symmetry is A. It is reasonable to assume thatupon protein binding, these bands shift to longer wavelengths due to thechanging microenvironment surrounding the polyene chain. It has beenwell established that CD spectra of carotenoids in which thechromophoric portions belong to the C₂ point group conform to theC₂-rule: if the overall conjugated system acquires right-handedchirality (i.e. dihedral angles around bonds 6-7 and 6′-7′ arenegative), then transitions of symmetry A lead to negative CE, andtransitions of symmetry B lead to positive CE (FIG. 8). Therefore, themeso-carotenoid binds to HSA in such a manner that the proteinenvironment fixes the terminal rings in a well-defined chiralconformation that results in the observed negative- and positive-inducedCD bands. The absolute configurations of the chiral 3 and 3′ centers donot determine the chiroptical property of the molecule; rather, theasymmetric protein environment of the albumin molecule (via non-covalentchemical interactions) determines the observed activity. In contrast tothe aggregate behavior in the aqueous solutions described above, theDAST molecules do not aggregate in HSA solution at these L/P ratios, asdemonstrated by the retention of the bell-shaped and slightlyred-shifted visible absorption band (FIG. 8). Thus, both the UV/Visabsorption and CD spectra indicate that the binding of themeso-carotenoid molecules to HSA occurs in monomeric form.

Optical Properties of dAST XVI in the Presence of HSA Above 1:1 L/PRatios

An increasing amount of dAST XVI was added to solutions of HSA preparedeither with pH 7.4 Ringer or 0.1 M pH 7.4 phosphate buffer to achieveL/P ratios higher than 1. Both CD and UV/Vis absorption spectraexhibited profound changes during addition of the ligand (FIG. 9 andFIG. 10). In addition to the blue-shifted visible absorption band a new,positive-negative CD band pair appeared around 480 and 420 nm,respectively. These CE's exhibited no vibrational fine structure andtheir amplitudes grew with increasing concentration of the ligand.However, there were some notable differences between the spectraobtained in the Ringer and phosphate buffer solutions:

-   -   a) The main absorption band shifted to lower wavelength (434.5        nm) in Ringer buffer. The corresponding value was 451.5 nm in        phosphate buffer.    -   b) Deviation of the zero cross-over point of CEs from the        maximum of the absorption band was three times larger in Ringer        (441.6 cm⁻¹) than phosphate buffer solution (148.4 cm⁻¹).    -   c) Above an L/P value of 8, the intensities of the CD bands no        longer increased in Ringer solution. In contrast, the        amplitude(s) of the CD bands continued to increase with        increasing L/P ratio in phosphate buffer, even at an L/P value        of 13.    -   d) At the same L/P ratios, more intense CD bands were measured        in phosphate buffer (FIG. 9 and FIG. 10).        The fact that these oppositely-signed CD bands appear only above        1:1 L/P ratio strongly suggests that they stemmed from chiral        intermolecular interactions between adjacent meso-carotenoid        molecules. When two electric transition dipole moments are        similar in energy, lie close to each other in space, and form a        chiral array, their interaction is manifested as chiral exciton        coupling: the CD spectrum shows a bisignate couplet matched with        the spectral position of the corresponding absorption band,        whose sign is determined by the absolute sense of twist between        the two dipoles. According to the exciton chirality rule, a        positive twist corresponds to a positive long-wavelength CE and        a negative CE at shorter wavelength, and vice versa. In this        case, the direction of the transition dipole moment is known; it        is polarized along the long axis of the polyene chain. Thus, the        neighboring meso-carotenoid molecules are arranged in such a        manner that their long axes form a positive (clockwise)        intermolecular overlay angle. Chiral arrangements of two        conjugated chains shown in FIG. 11 satisfy the former condition;        in these cases, a long-wavelength positive and a short        wavelength negative band would appear in the CD spectrum.        However, the spectroscopic behavior of the absorption band helps        to differentiate between these spatial arrangements. Due to        unfavourable Coulombic interactions between the transition        dipole moments of neighbouring meso-carotenoid molecules in the        case of a and b (FIG. 11), the absorption maximum shifts to        higher energies; if the c form exists, then the absorption band        widens and its maximum shifts to lower energies. Consequently,        dAST XVI molecules form a right-handed chiral array in which the        long axes of meso-carotenoid monomers form an acute, positive        angle (FIGS. 11 a and b).

The following scenario is proposed for the origin of the chiral orderingof the ligand molecules. Albumin appears necessary for the inducedoptical activity and, at first, it is tempting to assume that there is alarge binding site on HSA able to accommodate two meso-carotenoidmolecules. At low L/P values albumin would bind only a single ligand; athigher L/P concentrations, a second meso-carotenoid monomer would becomplexed. As stated above, however, the magnitudes of CEs continue toincrease at quite high L/P values (FIG. 10), in which case a singlebinding site should already be saturated. One resolution to this issueassumes that HSA is an asymmetric template on which the chiralself-assembly is started. The first few meso-carotenoid molecules bindto HSA in right-handed arrangement, and subsequent meso-carotenoidmonomers build upon this chiral architecture. In this scenario, HSAprovides the first essential step, the chiral initiation (“chiralseeding”); after this the self-assembly continues automatically. It isvery important to note, however, that without their chiral end-groupsonly a few dAST XVI molecules would be held in right-handed arrangementat the binding site of HSA. The 3 and 3′ chiral centers play a decisiverole in allowing the aggregates to form the chiral self-assembly on theHSA molecules. In the absence of protein, the meso-carotenoid moleculesform right- and left-handed assemblies to an equal extent, due to thelack of chiral discrimination.

As listed above, the spectral differences between the CD curves measuredin phosphate buffer and Ringer solutions suggested the influence of thesalt concentration on the stability of the aggregates (FIG. 9 and FIG.10). The osmolarity and ionic strength of the Ringer buffer was higherthan that of phosphate buffer. The succinic moieties were ionized at pH7.4 in both buffer solutions and electrostatic repulsion arose bothwithin and among the aggregates. Positively-charged salt ions are ableto decrease this repulsion, and therefore contribute to an increasingstability and size of the aggregates in the presence of these cations.During the titration of HSA with dAST XVI above the 1:1 L/P ratio, bothchiral and achiral aggregates were simultaneously formed; however, onlychiral aggregates were associated with HSA, while achiral aggregateswere not. CD spectra obtained in Ringer buffer solution (FIG. 9)suggested that the achiral aggregates were better stabilized in thishigher osmolarity buffer due to the screening effect of the salt ions.The added ligand molecules preferentially associated with existingaggregates, which resulted in the amplitudes of the CD bands reaching aplateau and becoming constant in contrast with the phosphate buffer.

Fluorescence Quenching of HSA Upon Addition of dAST

The single tryptophan residue (Trp214) located in the depth of subdomainIIA is largely responsible for the intrinsic fluorescence of HSA. Thefluorescence emission spectrum of HSA overlaps with the absorptionspectrum of the meso-carotenoid. Therefore, fluorescence spectroscopicmeasurements were obtained after incremental addition of dAST XVI inDMSO to a solution of HSA. The results clearly demonstrated that themeso-carotenoid molecules were able to effectively quench the intrinsicfluorescence of HSA (FIG. 12). The DMSO used to prepare the stocksolution of dAST XVI exhibited a negligible effect on the intrinsic HSAfluorescence (FIG. 12). At an L/P ratio of 0.7, the baselinefluorescence intensity decreased by 50%. The observed phenomenonsuggested that a meso-carotenoid molecule was bound in the vicinity ofTrp214, which forms part of the wall in one of the two main bindingcavities of HSA (site I, subdomain IIA; FIG. 13). However, neither siteI nor site II (subdomain IIIA)—both hydrophobic fatty acid bindingtunnels—are capable of accommodating the long, rigid dast XVI molecule(FIG. 13). Based on structural similarity, a second possibility is thatdAST XVI binds to other long-chain (C18, C20) fatty acid binding sitesof HSA, which have been well-characterized by high resolution X-raycrystallography. In the case of shorter, open-chain carotenoids havingno bulky end-groups, this possibility may be likely. However, thepolyene chain of the meso-carotenoid derivative itself measures 28 Å(between the 3 and 3′ chiral carbon atoms). Despite their conformationalmobility, the succinate moieties require additional space, increasingthe effective length of the molecule to 48 Å. Careful inspection of thecrystal structure of HSA suggests that the long, narrow cleft betweendomains I and III may be suitable for the binding of a meso-carotenoidcarotenoid molecule (FIG. 13). The interdomain cleft is wide, and itsnarrow end is close to the tryptophan (Trp214; * on FIG. 13) residuewhich would provide a structural explanation for the observedfluorescence quenching upon binding of the meso-carotenoid molecule tothe interdomain cleft of HSA. Furthermore, it may be assumed thatassociation of additional dast XVI molecules to the single one in theinterdomain cleft induces significant conformational changes of HSAresulting in the widening of the central crevice. This might be thereason why the fluorescence quenching did not stop at an L/P=1 ratio butkeeps on strengthening as the CEs increase (FIG. 13).

Discussion of UV/Vis and CD Spectroscopy Results

As a consequence of exclusion from the aqueous environment andintermolecular hydrogen bonding, the disodium salt disuccinatederivative XVI of synthetic, achiral meso-astaxanthin formed opticallyinactive, card-pack type aggregates in aqueous buffer solutions, asindicated by the large blue-shift of the main visible absorption bandversus the band observed in ethanolic solution. In the presence of anexcess of fatty acid-free HSA, the meso-carotenoid appears to bepreferentially associated with HSA in monomeric fashion. These resultssuggest that the weak van der Waal's forces and hydrogen bonding thatpermits supramolecular assembly in aqueous solution will be rapidlyovercome in a biologically relevant environment. The concentration ofalbumin in human blood in vivo is approximately 0.6 mM, suggesting thatat doses of up to 500 mg, the meso-carotenoid (molecular weight 841 Da)will associate with the albumin in monomeric fashion (excludingadditional potential non-specific binding to circulating blood cells andlipoproteins, which would increase the potential non-aggregating dose).Bound meso-carotenoid molecules exhibited induced CD bands which wereadequately explained by a right-handed helical conformation of theconjugated system. Graded fluorescence quenching of HSA in the presenceof increasing concentrations of dast XVI reinforced the notion thatformation of carotenoid-albumin complexes were responsible for thisquenching, and suggested spatial proximity between the bound ligand andthe tryptophan 214 residue of HSA. Based on the spectroscopic data, themolecular length of the dast XVI molecule, and the well-characterizedcrystal structure of HSA, the binding site was tentatively assigned tothe interdomain cleft located between domains I and III.

There appears to be a positive-negative band pair in the CD spectrumabove 1:1 L/P ratio of meso-carotenoid to HSA. This finding wasattributed to intermolacular chiral exciton coupling betweenmeso-carotenoid polyene chains arranged in right-handed assembly. Theexperimental data suggested that HSA acts as a chiral template on whichthe self-assembly begins, and subsequently continues governed by thechirality of the end-groups of the meso-carotenoid molecules. Thedifferences between bisignate CD spectra obtained in pH 7.4 phosphatebuffer and Ringer solutions indicate that the self-assembly isinfluenced by the osmolarity and ionic strength of the solution. Withincreasing osmolarity, the stability of the aggregates is enhancedpresumably due to the electrostatic screening of the negatively-chargedsuccinic carboxylate functions by salt cations.

In this patent, certain U.S. patents, U.S. patent applications, andother materials (e.g., articles) have been incorporated by reference.The text of such U.S. patents, U.S. patent applications, and othermaterials is, however, only incorporated by reference to the extent thatno conflict exists between such text and the other statements anddrawings set forth herein. In the event of such conflict, then any suchconflicting text in such incorporated by reference U.S. patents, U.S.patent applications, and other materials is specifically notincorporated by reference in this patent.

Further modifications and alternative embodiments of various aspects ofthe invention will be apparent to those skilled in the art in view ofthis description. Accordingly, this description is to be construed asillustrative only and is for the purpose of teaching those skilled inthe art the general manner of carrying out the invention. It is to beunderstood that the forms of the invention shown and described hereinare to be taken as the presently preferred embodiments. Elements andmaterials may be substituted for those illustrated and described herein,parts and processes may be reversed, and certain features of theinvention may be utilized independently, all as would be apparent to oneskilled in the art after having the benefit of this description of theinvention. Changes may be made in the elements described herein withoutdeparting from the spirit and scope of the invention as described in thefollowing claims.

REFERENCES

The following references are specifically incorporated herein byreference:

U.S. Patent Documents 5871766 Feb., 1999 Hennekens

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1. A method of treating cardiac arrhythmia in a subject comprisingadministering to the subject who would benefit from such treatment aneffective amount of a pharmaceutically acceptable formulation toincrease connexin 43 expression in at least the a portion of themyocardium of the subject, said pharmaceutically acceptable formulationcomprising a synthetic carotenoid analog or derivative; wherein thesynthetic carotenoid analog or derivative has the structure

wherein y is from 5 to 12; wherein each R³ is independently hydrogen ormethyl; and where each R¹ and R² are independently:

wherein each substituent —W is independently —OX —O(CO)R, wherein each—X is independently

 -alkyl-NR⁵ ₃ ⁺, aryl-NR⁵ ₃ ⁺, -alkyl-CO₂ ⁻, -aryl-CO₂ ⁻, -aminoacid-NH₃ ⁺, -alkyl-O—PO₂ ⁻, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, benzyl

wherein each R⁴ is independently -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺,-aryl-CO₂, -amino acid —NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, H, alkyl, aryl, or alkali salt; whereineach R⁵ is independently H, alkyl, or aryl; wherein each R⁶ isindependently H, alkyl, benzyl, or alkali salt; wherein each —R isindependently -alkyl-NR⁵ ₃ ⁺, -alkyl-NR⁵ ₂, -aryl-NR⁵ ₃ ⁺, -alkyl-CO₂ ⁻,-alkyl-CO₂H, -alkyl-CO₂R⁷, -alkyl-CO₂R⁸, —OR⁸, -aryl-CO₂ ⁻, -aminoacid-NH₂, -amino acid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, H, or aryl, -alkyl-O—PO₂-O-alkyl-NR⁵ ₃ ⁺,

wherein R' is -alkyl-O, alkyl, or aryl; wherein R⁷ is—CH₂—CH(OH)—CH₂—O—PO₂—O-alkyl-NR⁵ ₃ ⁺,

and wherein R⁸ is -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺, -aryl-CO₂ ⁻, -aminoacid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺, polyethylene glycol,dextran, H, alkyl

 or aryl.
 2. A method of inhibiting or reducing the growth of acancerous or a precancerous cell mass in a subject comprisingadministering to a subject in need thereof an effective amount of apharmaceutically acceptable formulation to increase the number of gapjunctions in at least a portion of the cells comprising the cell mass,said pharmaceutically acceptable formulation comprising a syntheticcarotenoid analog or derivative; wherein the synthetic carotenoid analogor derivative has the structure:

wherein y is from 5 to 12 wherein each R³ is independently hydrogen ormethyl; where each R¹ and R² are independently:

wherein each substituent —W is independently —OX or —O(CO)R, whereineach —R is independently

 -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺, -alkyl-CO₂ ⁻, -amino acid-NH₃ ⁺,-alkyl-O—PO₂ ⁻, -phosphorylated amino acid-NH₃ ⁻, polyethylene glycol,dextran, benzyl,

wherein each R⁴ is independently -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺,-aryl-CO₂ ⁻, -amino acid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, H, alkyl, aryl, or alkali salt; whereineach R⁵ is independently H, alkyl, or aryl; wherein each R⁶ isindependently H, alkyl, benzyl, or alkali salt; wherein each —R isindependently -alkyl-NR⁵ ₃ ⁺, -alkyl-NR⁵ ₂, -aryl-NR⁵ ₃ ⁺, -alkyl-CO₂ ⁻,-alkyl-CO₂H, -alkyl-CO₂R⁷, -alkyl-CO₂R⁸, —OR⁸, -aryl-CO₂ ⁻, -aminoacid-NH₂, -amino acid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, H, or aryl, -alkyl-O—PO₂—O-alkyl-NR⁵ ₃ ⁺,

wherein R' is -alkyl-O, alkyl, or aryl; wherein R⁷ is—CH₂—CH(OH)—CH₂—O—PO₂—O-alkyl-NR⁵ ₃ ⁺,

wherein R⁸ is -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺, -aryl-CO₂ ⁻, -aminoacid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺, polyethylene glycol,dextran, H, alkyl,

 or aryl.
 3. A method of treating a solid tumor in a subject comprisingadministering to a subject who would benefit such treatment an effectiveamount of a pharmaceutically acceptable formulation to increase connexin43 expression in at least a portion of the cells comprising the solidtumor, said pharmaceutically acceptable formulation comprising asynthetic carotenoid analog or derivative; wherein the synthetic analogor derivative of the carotenoid has the structure

wherein y is from 5 to 12 wherein each R³ is independently hydrogen ormethyl; and where each R¹ and R² are independently:

wherein each substituent —W is independently —OX or O(CO)R, wherein each—R is independently

 -alky -NR⁵ ₃₊, -aryl-NR⁵ ₃ ⁺, -alkyl-CO₂ ⁻, -aryl-CO₂ ⁻, -aminoacid-NH₃ ⁺, -alkyl-O—PO₂ ⁻, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, benzyl,

wherein each R⁴ is independently -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺,-alkyl-CO₂ ⁻, -aryl-CO₂ ⁻, -amino acid-NH₃ ⁺, -phosphorylated aminoacid-NH₃ ^(÷), polyethylene glycol, dextran, H, alkyl, aryl, or alkalisalt; wherein each R⁵ is independently H, alkyl, or aryl; wherein eachR⁶ is independently H, alkyl, benzyl, or alkali salt; wherein each —R isindependently -alkyl-NR⁵ ₃ ⁺, -alkyl-NR⁵ ₂, -aryl-NR⁵ ₃ ⁺, -alkyl-CO² ⁻,-alkyl-CO₂H, -alkyl-CO₂R⁷, -alkyl-CO₂R⁸, —OR⁸, -aryl-CO₂ ⁻, -aminoacid-NH₂, -amino acid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, H, or aryl, -alkyl-O—PO₂—O-alkyl-NR⁵ ₃ ⁺,

wherein R' is -alkyl-O, alkyl, or aryl; wherein R⁷ is—CH₂—CH(OH)—CH₂—O—PO₂—O-alkyl-NR⁵ ₃ ⁺,

and wherein R⁸ is -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺, -aryl-CO₂ ⁻, -aminoacid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺, polyethylene glycol,dextran, H, alkyl,

 or aryl.
 4. The method of claim 3, wherein the increase in connexin 43expression in the cells is associated with a reduction in the growthrate of least a portion of the cells comprising the solid tumor.
 5. Themethod of claim 3, the increase in connexin 43 expression in the cellsis associated with an increase in intercellular gap junctionalcommunication between at least a portion of the cells comprising thesolid tumor.
 6. The method of claim 3, wherein the subject is a mammal.7. The method of claim 3, wherein the subject is human.
 8. The method ofclaim 3, wherein the pharmaceutically acceptable formulation isadministered to the subject parenterally.
 9. The method of claim 3,wherein the pharmaceutically acceptable formulation is administered tothe subject parenterally at a dose of about 5 mg to about 300 mg perday.
 10. The method of claim 3, wherein the carotenoid analog orderivative is administered to the subject parenterally at a dose ofabout 0.25 mg to about 1.0 g per day.
 11. The method of claim 3, whereinthe pharmaceutically acceptable formulation is administered to thesubject subcutaneously.
 12. The method of claim 3, wherein thepharmaceutically acceptable formulation is administered to the subjectorally.
 13. The method of claim 3, wherein the pharmaceuticallyacceptable formulation is administered to the subject orally at a doseof about 5 mg to about 100 mg per day.
 14. The method of claim 3,wherein the pharmaceutically acceptable formulation is administered tothe subject orally at a dose of about 0.25 mg to about 1.0 g per day.15. The method of claim 3, wherein the pharmaceutically acceptableformulation is administered to the subject at a dose of about 0.25 mg toabout 1 g.
 16. The method of claim 3, wherein at least two differentcarotenoid analog or derivatives are administered to the subject. 17.The method of claim 3, wherein at least one substituent, —W, isindependently

where each R is independently -alkyl-NR⁴ ₃ ⁺, -aryl-NR⁴ ₃ ⁺, -alkyl-CO₂⁻, -aryl-CO₂ ⁻, -amino acid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺,polyethylene glycol, dextran, H, alkyl, or aryl; and where each R⁴ isindependently H, alkyl, or aryl.
 18. The method of claim 3, wherein thecarotenoid analog or derivative has the structure:

where each R¹ and R² are independently:

where —W is —OH or OX, where at least one —W is OX, where X is

 and where R⁴ is -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺, -alkyl-CO₂ ⁻, -aryl-CO₂⁻, -amino acid-NH₃ ⁺, -phosphorylated amino acid-NH₃ ⁺, polyethyleneglycol, dextran, H, alkyl, aryl, or alkali salt.
 19. The method of claim3, wherein the carotenoid analog or derivative has the structure:

where each R¹ and R² are independently:

wherein —W is —OH or O(CO)R, where at least one —W is O(CO)R, where —Ris

wherein R' is -alkyl-O, alkyl, or aryl; where R⁸ is -alkyl-NR⁵ ₃ ⁺,-aryl-NR⁵ ₃ ⁺, -aryl-CO₂ ⁻, -amino acid-NH₃ ⁺, -phosphorylated aminoacid-NH₃ ⁺, polyethylene glycol, dextran, H, alkyl,

 or aryl; where each R⁵ is independently H, alkyl, or aryl.
 20. Themethod of claim 3, wherein the carotenoid analog or derivative has thestructure:

where each R¹ and R² are independently:

where —W is —OH or OX, where at least one —W is OX, where —X is

wherein each R⁴ is independently -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺,-alkyl-CO₂ ⁻, -aryl-CO₂ ⁻, -amino acid-NH₃ ⁺, -phosphorylated aminoacid-NH₃ ⁺, polyethylene glycol, dextran, H, alkyl, aryl, or alkalisalt; and where each R⁵ is independently H, alkyl, or aryl.
 21. Themethod of claim 3, wherein the carotenoid analog or derivative has thestructure:

where each R¹ and R² are independently:

where —W is —OH or O(CO)R, where at least one —W is O(CO)R, where —R is-alkyl-CO₂ ⁻, -alkyl-CO₂H, -alkyl-CO₂R⁹, where R⁹ is alkyl,—CH₂—CH(OH)—CH₂—O—PO₂—O-alkyl-NR⁵ ₃ ⁺,

 -alkyl-NR⁵ ₃ ⁺, -aryl-NR⁵ ₃ ⁺, -amino acid-NH₃ ⁺, -phosphorylated aminoacid-NH₃ ⁺, polyethylene glycol, dextran, H, alkyl, or aryl, where eachR⁵ is independently H, alkyl, or aryl.
 22. The method of claim 3,wherein the carotenoid analog or derivative has the structure:

where each R¹ and R² are independently:

where —W is —OH or O(CO)R, where at least one —W is —O(CO)R, where eachR is

where R⁶ independently H, alkyl, benzyl, or alkali salt.
 23. The methodof claim 3, wherein the carotenoid analog or derivative has thestructure:

where each R¹ and R² are independently:

where —W is —OH or —OX, where at least one —W is —OX, where X is

where each R⁶ is independently H, alkyl, benzyl, or alkali salt.
 24. Themethod of claim 3, wherein the carotenoid analog or derivative has thestructure:

where each R¹ and R² are independently:

where —W is —OH or —OC(O)-alkyl-CO₂R⁹, where at least one —W is—OC(O)-alkyl-CO₂R⁹, where R⁹ is

 and where each R⁶ is independently H, alkyl, benzyl, or alkali salt.25. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH,

where at least one —W is


26. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or

where at least one —W is


27. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or —O(CO), where at least one —W is —O(CO), where R is-amino acid-NH₂ or -amino acid-NH₃ ⁺.
 28. The method of claim 3, whereinthe carotenoid analog or derivative has the structure:

where each R¹ and R² are independently:

where —W is —OH or —O(CO)R, where at least one —W is —O(CO)R, where —Ris


29. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or —O(CO)R, where at least one —W is —O(CO)R, where —Ris -alkyl-NR⁵ ₃ ⁺, -alkyl-NR⁵ ₂, -aryl-NR⁵ ₃ ⁺, where each R⁵ isindependently H, alkyl, or aryl.
 30. The method of claim 3, wherein thecarotenoid analog or derivative has the structure:

where each R¹ and R² are independently:

where —W is —OH or,

 where at least one —W is


31. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or,

 where at least one —W is


32. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or benzyl, where at least one —W is benzyl.
 33. Themethod of claim 3, wherein the carotenoid analog or derivative has thestructure:

where each R¹ and R² are independently:

where —W is —OH or,

 where at least one —W is


34. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or,

 where at least one —W is


35. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or,

 where at least one —W is


36. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or,

 where at least one —W is


37. The method of claim 3, wherein the carotenoid analog or derivativehas the structure:

where each R¹ and R² are independently:

where —W is —OH or,

 where at least one —W is


38. The method of claim 3, wherein the carotenoid analog or derivativehas the structure

or a pharmaceutically acceptable salt thereof.
 39. The method of claim3, wherein the pharmaceutically acceptable formulation is administeredto the subject in the form of an emulsion.
 40. The method of claim 3,wherein the pharmaceutically acceptable formulation is administered tothe subject in the form of an emulsion comprising water, oil andlecithin.
 41. The method of claim 3, further comprising administering aco-antioxidant.
 42. The method of claim 3, wherein the pharmaceuticallyacceptable formulation is administered to a subject by intracoronaryadministration.
 43. The method of claim 3, wherein the pharmaceuticallyacceptable formulation is administered to a subject at a dose of about 5mg to about 300 mg per day by intracoronary administration.
 44. Themethod of claim 3, wherein the pharmaceutically acceptable formulationis administered to a subject at a dose of about 0.25 mg to about 1.0 gper day.
 45. The method of claim 3, wherein the carotenoid analog orderivative has the structure